Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shifts
Thiabendazole (TBZ), a recalcitrant fungicide, is frequently applied in postharvest fruit treatment and generates significant volumes of industrial wastewater (WW) that conventional treatment plants cannot handle. This explores a bioelectrochemical system (BES) for TBZ degradation using Tunisian hyp...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1529841/full |
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| author | Nesrine Saidi Benjamin Erable Luc Etchevery Ameur Cherif Habib Chouchane |
| author_facet | Nesrine Saidi Benjamin Erable Luc Etchevery Ameur Cherif Habib Chouchane |
| author_sort | Nesrine Saidi |
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| description | Thiabendazole (TBZ), a recalcitrant fungicide, is frequently applied in postharvest fruit treatment and generates significant volumes of industrial wastewater (WW) that conventional treatment plants cannot handle. This explores a bioelectrochemical system (BES) for TBZ degradation using Tunisian hypersaline sediments (THSs) as inoculum. Four sets of BES, along with biological controls, were tested using THS subjected to different levels of TBZ biostimulation. Sediments underwent one, two, or three biostimulation phases with increasing TBZ concentrations (0, 10, 100, and 300 mg kg−1). Potentiostatic control was applied to BES, polarized at 0.1 V vs. saturated calomel reference electrode (SCE), with a carbon felt working electrode (72 cm2 L−1) and maintained at 25°C. While current production was very low, sediments biostimulated with 100 mg kg−1 kg TBZ produced the highest current density (3.2 mA m−2), a 5-fold increase over untreated sediments (0.6 mA m−2). GC-FID analysis showed >99% TBZ degradation in all reactors. The TBZ half-elimination time from 27 days with biological treatments to 19 days in BES and further to 6 days following biostimulation. Bacterial analysis revealed a substantial microbial community shift after biostimulation, with a reduction in Bacillota (−64%) and an increase in Proteobacteria (+62%), dominated by Pseudomonas (45%) and Marinobacter (16%). These findings provide insight into the selective potential of biostimulation cycles to enhance microbial community composition and improve BES performance for TBZ wastewater treatment. |
| format | Article |
| id | doaj-art-3fa7f0bd98464fb69f2d414d07261c50 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-3fa7f0bd98464fb69f2d414d07261c502025-01-06T06:59:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.15298411529841Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shiftsNesrine Saidi0Benjamin Erable1Luc Etchevery2Ameur Cherif3Habib Chouchane4ISBST, BVBGR-LR11ES31, Biotechpole Sidi Thabet, University of Manouba, Ariana, TunisiaLaboratoire de Génie Chimique, CNRS, INPT, UPS, Université de Toulouse, Toulouse, FranceLaboratoire de Génie Chimique, CNRS, INPT, UPS, Université de Toulouse, Toulouse, FranceISBST, BVBGR-LR11ES31, Biotechpole Sidi Thabet, University of Manouba, Ariana, TunisiaISBST, BVBGR-LR11ES31, Biotechpole Sidi Thabet, University of Manouba, Ariana, TunisiaThiabendazole (TBZ), a recalcitrant fungicide, is frequently applied in postharvest fruit treatment and generates significant volumes of industrial wastewater (WW) that conventional treatment plants cannot handle. This explores a bioelectrochemical system (BES) for TBZ degradation using Tunisian hypersaline sediments (THSs) as inoculum. Four sets of BES, along with biological controls, were tested using THS subjected to different levels of TBZ biostimulation. Sediments underwent one, two, or three biostimulation phases with increasing TBZ concentrations (0, 10, 100, and 300 mg kg−1). Potentiostatic control was applied to BES, polarized at 0.1 V vs. saturated calomel reference electrode (SCE), with a carbon felt working electrode (72 cm2 L−1) and maintained at 25°C. While current production was very low, sediments biostimulated with 100 mg kg−1 kg TBZ produced the highest current density (3.2 mA m−2), a 5-fold increase over untreated sediments (0.6 mA m−2). GC-FID analysis showed >99% TBZ degradation in all reactors. The TBZ half-elimination time from 27 days with biological treatments to 19 days in BES and further to 6 days following biostimulation. Bacterial analysis revealed a substantial microbial community shift after biostimulation, with a reduction in Bacillota (−64%) and an increase in Proteobacteria (+62%), dominated by Pseudomonas (45%) and Marinobacter (16%). These findings provide insight into the selective potential of biostimulation cycles to enhance microbial community composition and improve BES performance for TBZ wastewater treatment.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1529841/fullbioremediationbacterial shiftsmicrobial degradationmicrobial communityrecalcitrant organic compoundbioelectrodegradation |
| spellingShingle | Nesrine Saidi Benjamin Erable Luc Etchevery Ameur Cherif Habib Chouchane Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shifts Frontiers in Microbiology bioremediation bacterial shifts microbial degradation microbial community recalcitrant organic compound bioelectrodegradation |
| title | Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shifts |
| title_full | Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shifts |
| title_fullStr | Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shifts |
| title_full_unstemmed | Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shifts |
| title_short | Enhanced bioelectrochemical degradation of Thiabendazole using biostimulated Tunisian hypersaline sediments: kinetics, efficiency, and microbial community shifts |
| title_sort | enhanced bioelectrochemical degradation of thiabendazole using biostimulated tunisian hypersaline sediments kinetics efficiency and microbial community shifts |
| topic | bioremediation bacterial shifts microbial degradation microbial community recalcitrant organic compound bioelectrodegradation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1529841/full |
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