Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria

Identification of the diverse animal hosts responsible for spill-over events from animals to humans is crucial for comprehending the transmission patterns of emerging infectious diseases, which pose significant public health risks. To better characterize potential animal hosts of Lassa virus (LASV),...

Full description

Saved in:
Bibliographic Details
Main Authors: Anise Nkenjop Happi, Olusola Akinola Ogunsanya, Akeemat Opeyemi Ayinla, Ayotunde Elijah Sijuwola, Femi Mudasiru Saibu, Kazeem Akano, Cecilia Nwofoke, Obineche Tobias Elias, Olivia Achonduh-Atijegbe, Richard Olumide Daodu, Oluwatobi Abel Adedokun, Abraham Adeyemo, Kehinde Ebenezer Ogundana, Omolola Zaheedat Lawal, Edyth Parker, Iguosadolo Nosamiefan, Johnson Okolie, Zahra F. Parker, Melanie D. McCauley, Leigh Anne Eller, Kara Lombardi, Abdulwasiu Bolaji Tiamiyu, Michael Iroezindu, Edward Akinwale, Thierry Lamare Fouapon Assedi Njatou, Tsedal Mebrahtu, Erica Broach, Anastasia Zuppe, Petra Prins, Jenny Lay, Mihret Amare, Kayvon Modjarrad, Natalie D. Collins, Sandhya Vasan, Cynthia Tucker, Sharon Daye, Christian Tientcha Happi
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Emerging Microbes and Infections
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2023.2294859
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1846138745995132928
author Anise Nkenjop Happi
Olusola Akinola Ogunsanya
Akeemat Opeyemi Ayinla
Ayotunde Elijah Sijuwola
Femi Mudasiru Saibu
Kazeem Akano
Cecilia Nwofoke
Obineche Tobias Elias
Olivia Achonduh-Atijegbe
Richard Olumide Daodu
Oluwatobi Abel Adedokun
Abraham Adeyemo
Kehinde Ebenezer Ogundana
Omolola Zaheedat Lawal
Edyth Parker
Iguosadolo Nosamiefan
Johnson Okolie
Zahra F. Parker
Melanie D. McCauley
Leigh Anne Eller
Kara Lombardi
Abdulwasiu Bolaji Tiamiyu
Michael Iroezindu
Edward Akinwale
Thierry Lamare Fouapon Assedi Njatou
Tsedal Mebrahtu
Erica Broach
Anastasia Zuppe
Petra Prins
Jenny Lay
Mihret Amare
Kayvon Modjarrad
Natalie D. Collins
Sandhya Vasan
Cynthia Tucker
Sharon Daye
Christian Tientcha Happi
author_facet Anise Nkenjop Happi
Olusola Akinola Ogunsanya
Akeemat Opeyemi Ayinla
Ayotunde Elijah Sijuwola
Femi Mudasiru Saibu
Kazeem Akano
Cecilia Nwofoke
Obineche Tobias Elias
Olivia Achonduh-Atijegbe
Richard Olumide Daodu
Oluwatobi Abel Adedokun
Abraham Adeyemo
Kehinde Ebenezer Ogundana
Omolola Zaheedat Lawal
Edyth Parker
Iguosadolo Nosamiefan
Johnson Okolie
Zahra F. Parker
Melanie D. McCauley
Leigh Anne Eller
Kara Lombardi
Abdulwasiu Bolaji Tiamiyu
Michael Iroezindu
Edward Akinwale
Thierry Lamare Fouapon Assedi Njatou
Tsedal Mebrahtu
Erica Broach
Anastasia Zuppe
Petra Prins
Jenny Lay
Mihret Amare
Kayvon Modjarrad
Natalie D. Collins
Sandhya Vasan
Cynthia Tucker
Sharon Daye
Christian Tientcha Happi
author_sort Anise Nkenjop Happi
collection DOAJ
description Identification of the diverse animal hosts responsible for spill-over events from animals to humans is crucial for comprehending the transmission patterns of emerging infectious diseases, which pose significant public health risks. To better characterize potential animal hosts of Lassa virus (LASV), we assessed domestic and non-domestic animals from 2021–2022 in four locations in southern Nigeria with reported cases of Lassa fever (LF). Birds, lizards, and domestic mammals (dogs, pigs, cattle and goats) were screened using RT-qPCR, and whole genome sequencing was performed for lineage identification on selected LASV positive samples. Animals were also screened for exposure to LASV by enzyme-linked immunosorbent assay (ELISA). Among these animals, lizards had the highest positivity rate by PCR. Genomic sequencing of samples in most infected animals showed sub-lineage 2 g of LASV. Seropositivity was highest among cattle and lowest in pigs. Though the specific impact these additional hosts may have in the broader virus-host context are still unknown – specifically relating to pathogen diversity, evolution, and transmission – the detection of LASV in non-rodent hosts living in proximity to confirmed human LF cases suggests their involvement during transmission as potential reservoirs. Additional epidemiological data comparing viral genomes from humans and animals, as well as those circulating within the environment will be critical in understanding LASV transmission dynamics and will ultimately guide the development of countermeasures for this zoonotic health threat.
format Article
id doaj-art-3f94ef23e9a442aa9335d369ac1f2213
institution Kabale University
issn 2222-1751
language English
publishDate 2024-12-01
publisher Taylor & Francis Group
record_format Article
series Emerging Microbes and Infections
spelling doaj-art-3f94ef23e9a442aa9335d369ac1f22132024-12-07T04:40:16ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512024-12-0113110.1080/22221751.2023.2294859Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern NigeriaAnise Nkenjop Happi0Olusola Akinola Ogunsanya1Akeemat Opeyemi Ayinla2Ayotunde Elijah Sijuwola3Femi Mudasiru Saibu4Kazeem Akano5Cecilia Nwofoke6Obineche Tobias Elias7Olivia Achonduh-Atijegbe8Richard Olumide Daodu9Oluwatobi Abel Adedokun10Abraham Adeyemo11Kehinde Ebenezer Ogundana12Omolola Zaheedat Lawal13Edyth Parker14Iguosadolo Nosamiefan15Johnson Okolie16Zahra F. Parker17Melanie D. McCauley18Leigh Anne Eller19Kara Lombardi20Abdulwasiu Bolaji Tiamiyu21Michael Iroezindu22Edward Akinwale23Thierry Lamare Fouapon Assedi Njatou24Tsedal Mebrahtu25Erica Broach26Anastasia Zuppe27Petra Prins28Jenny Lay29Mihret Amare30Kayvon Modjarrad31Natalie D. Collins32Sandhya Vasan33Cynthia Tucker34Sharon Daye35Christian Tientcha Happi36African Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAlex Ekwueme Federal University Teaching Hospital, Abakaliki, Ebonyi State, NigeriaUniversity of Nigeria, Nsukka, NigeriaAlex Ekwueme Federal University Teaching Hospital, Abakaliki, Ebonyi State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaRedeemer’s University, Ede, Osun, NigeriaRedeemer’s University, Ede, Osun, NigeriaScripps Translational Science Institute, La Jolla, CA, USAAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation Medical Research International Ltd/Gte, Abuja, NigeriaHenry M. Jackson Foundation Medical Research International Ltd/Gte, Abuja, NigeriaHenry M. Jackson Foundation Medical Research International Ltd/Gte, Abuja, NigeriaOne Health Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAEmerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAViral Diseases Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USAOne Health Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAOne Health Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAAfrican Centre of Excellence for Genomics of Infectious Diseases, Redeemer’s University, Ede, Osun State, NigeriaIdentification of the diverse animal hosts responsible for spill-over events from animals to humans is crucial for comprehending the transmission patterns of emerging infectious diseases, which pose significant public health risks. To better characterize potential animal hosts of Lassa virus (LASV), we assessed domestic and non-domestic animals from 2021–2022 in four locations in southern Nigeria with reported cases of Lassa fever (LF). Birds, lizards, and domestic mammals (dogs, pigs, cattle and goats) were screened using RT-qPCR, and whole genome sequencing was performed for lineage identification on selected LASV positive samples. Animals were also screened for exposure to LASV by enzyme-linked immunosorbent assay (ELISA). Among these animals, lizards had the highest positivity rate by PCR. Genomic sequencing of samples in most infected animals showed sub-lineage 2 g of LASV. Seropositivity was highest among cattle and lowest in pigs. Though the specific impact these additional hosts may have in the broader virus-host context are still unknown – specifically relating to pathogen diversity, evolution, and transmission – the detection of LASV in non-rodent hosts living in proximity to confirmed human LF cases suggests their involvement during transmission as potential reservoirs. Additional epidemiological data comparing viral genomes from humans and animals, as well as those circulating within the environment will be critical in understanding LASV transmission dynamics and will ultimately guide the development of countermeasures for this zoonotic health threat.https://www.tandfonline.com/doi/10.1080/22221751.2023.2294859Lassa virusLASV genomesnon-rodentsanimalsNigeriaLASV lineages 2 g
spellingShingle Anise Nkenjop Happi
Olusola Akinola Ogunsanya
Akeemat Opeyemi Ayinla
Ayotunde Elijah Sijuwola
Femi Mudasiru Saibu
Kazeem Akano
Cecilia Nwofoke
Obineche Tobias Elias
Olivia Achonduh-Atijegbe
Richard Olumide Daodu
Oluwatobi Abel Adedokun
Abraham Adeyemo
Kehinde Ebenezer Ogundana
Omolola Zaheedat Lawal
Edyth Parker
Iguosadolo Nosamiefan
Johnson Okolie
Zahra F. Parker
Melanie D. McCauley
Leigh Anne Eller
Kara Lombardi
Abdulwasiu Bolaji Tiamiyu
Michael Iroezindu
Edward Akinwale
Thierry Lamare Fouapon Assedi Njatou
Tsedal Mebrahtu
Erica Broach
Anastasia Zuppe
Petra Prins
Jenny Lay
Mihret Amare
Kayvon Modjarrad
Natalie D. Collins
Sandhya Vasan
Cynthia Tucker
Sharon Daye
Christian Tientcha Happi
Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria
Emerging Microbes and Infections
Lassa virus
LASV genomes
non-rodents
animals
Nigeria
LASV lineages 2 g
title Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria
title_full Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria
title_fullStr Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria
title_full_unstemmed Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria
title_short Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria
title_sort lassa virus in novel hosts insights into the epidemiology of lassa virus infections in southern nigeria
topic Lassa virus
LASV genomes
non-rodents
animals
Nigeria
LASV lineages 2 g
url https://www.tandfonline.com/doi/10.1080/22221751.2023.2294859
work_keys_str_mv AT anisenkenjophappi lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT olusolaakinolaogunsanya lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT akeematopeyemiayinla lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT ayotundeelijahsijuwola lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT femimudasirusaibu lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT kazeemakano lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT cecilianwofoke lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT obinechetobiaselias lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT oliviaachonduhatijegbe lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT richardolumidedaodu lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT oluwatobiabeladedokun lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT abrahamadeyemo lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT kehindeebenezerogundana lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT omololazaheedatlawal lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT edythparker lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT iguosadolonosamiefan lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT johnsonokolie lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT zahrafparker lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT melaniedmccauley lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT leighanneeller lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT karalombardi lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT abdulwasiubolajitiamiyu lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT michaeliroezindu lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT edwardakinwale lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT thierrylamarefouaponassedinjatou lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT tsedalmebrahtu lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT ericabroach lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT anastasiazuppe lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT petraprins lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT jennylay lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT mihretamare lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT kayvonmodjarrad lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT nataliedcollins lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT sandhyavasan lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT cynthiatucker lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT sharondaye lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria
AT christiantientchahappi lassavirusinnovelhostsinsightsintotheepidemiologyoflassavirusinfectionsinsouthernnigeria