Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity
Abstract Multi-walled Carbon Nanotubes (MWCNTs) are inert structures with high aspect ratios that are widely used as vehicles for targeted drug delivery in cancer and many other diseases. They are largely non-toxic in nature however, when cells are exposed to these nanotubes for prolonged durations...
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| Format: | Article |
| Language: | English |
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Springer
2024-03-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06789-9 |
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| author | Samiksha Rele Chanchal Kiran Thakur Fatima Khan Budhadev Baral Vaishali Saini Chandrabose Karthikeyan N. S. Hari Narayana Moorthy Hem Chandra Jha |
| author_facet | Samiksha Rele Chanchal Kiran Thakur Fatima Khan Budhadev Baral Vaishali Saini Chandrabose Karthikeyan N. S. Hari Narayana Moorthy Hem Chandra Jha |
| author_sort | Samiksha Rele |
| collection | DOAJ |
| description | Abstract Multi-walled Carbon Nanotubes (MWCNTs) are inert structures with high aspect ratios that are widely used as vehicles for targeted drug delivery in cancer and many other diseases. They are largely non-toxic in nature however, when cells are exposed to these nanotubes for prolonged durations or at high concentrations, they show certain adverse effects. These include cytotoxicity, inflammation, generation of oxidative stress, and genotoxicity among others. To combat such adverse effects, various moieties can be attached to the surface of these nanotubes. Curcumin is a known anti-inflammatory, antioxidant and cytoprotective compound derived from a medicinal plant called Curcuma longa. In this study, we have synthesized and characterized Curcumin coated-lysine functionalized MWCNTs and further evaluated the cytoprotective, anti-inflammatory, antioxidant and antiapoptotic effect of Curcumin coating on the surface of MWCNTs. The results show a significant decrease in the level of inflammatory molecules like IL-6, IL-8, IL-1β, TNFα and NFκB in cells exposed to Curcumin-coated MWCNTs as compared to the uncoated ones at both transcript and protein levels. Further, compared to the uncoated samples, there is a reduction in ROS production and upregulation of antioxidant enzyme-Catalase in the cells treated with Curcumin-coated MWCNTs. Curcumin coating also helped in recovery of mitochondrial membrane potential in the cells exposed to MWCNTs. Lastly, cells exposed to Curcumin-coated MWCNTs showed reduced cell death as compared to the ones exposed to uncoated MWCNTs. Our findings suggest that coating of Curcumin on the surface of MWCNTs reduces its ability to cause inflammation, oxidative stress, and cell death. Graphical Abstract (a) Synthesis of Curcumin-coated-Lysine-functionalized MWCNTs. (b) Flow of research depicting experimental groups and studies performed along with the underlying techniques used. |
| format | Article |
| id | doaj-art-3f3d06dec4c145ba9ad9de0e8ad3a4ff |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-3f3d06dec4c145ba9ad9de0e8ad3a4ff2024-12-22T12:10:38ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382024-03-0135111710.1007/s10856-024-06789-9Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicitySamiksha Rele0Chanchal Kiran Thakur1Fatima Khan2Budhadev Baral3Vaishali Saini4Chandrabose Karthikeyan5N. S. Hari Narayana Moorthy6Hem Chandra Jha7Infection Bioengineering Group, Department of Biosciences and Biomedical Engineering, Indian Institute of Technology IndoreCancept Therapeutics Laboratory, Department of Pharmacy, Indira Gandhi National Tribal UniversityCancept Therapeutics Laboratory, Department of Pharmacy, Indira Gandhi National Tribal UniversityInfection Bioengineering Group, Department of Biosciences and Biomedical Engineering, Indian Institute of Technology IndoreInfection Bioengineering Group, Department of Biosciences and Biomedical Engineering, Indian Institute of Technology IndoreCancept Therapeutics Laboratory, Department of Pharmacy, Indira Gandhi National Tribal UniversityCancept Therapeutics Laboratory, Department of Pharmacy, Indira Gandhi National Tribal UniversityInfection Bioengineering Group, Department of Biosciences and Biomedical Engineering, Indian Institute of Technology IndoreAbstract Multi-walled Carbon Nanotubes (MWCNTs) are inert structures with high aspect ratios that are widely used as vehicles for targeted drug delivery in cancer and many other diseases. They are largely non-toxic in nature however, when cells are exposed to these nanotubes for prolonged durations or at high concentrations, they show certain adverse effects. These include cytotoxicity, inflammation, generation of oxidative stress, and genotoxicity among others. To combat such adverse effects, various moieties can be attached to the surface of these nanotubes. Curcumin is a known anti-inflammatory, antioxidant and cytoprotective compound derived from a medicinal plant called Curcuma longa. In this study, we have synthesized and characterized Curcumin coated-lysine functionalized MWCNTs and further evaluated the cytoprotective, anti-inflammatory, antioxidant and antiapoptotic effect of Curcumin coating on the surface of MWCNTs. The results show a significant decrease in the level of inflammatory molecules like IL-6, IL-8, IL-1β, TNFα and NFκB in cells exposed to Curcumin-coated MWCNTs as compared to the uncoated ones at both transcript and protein levels. Further, compared to the uncoated samples, there is a reduction in ROS production and upregulation of antioxidant enzyme-Catalase in the cells treated with Curcumin-coated MWCNTs. Curcumin coating also helped in recovery of mitochondrial membrane potential in the cells exposed to MWCNTs. Lastly, cells exposed to Curcumin-coated MWCNTs showed reduced cell death as compared to the ones exposed to uncoated MWCNTs. Our findings suggest that coating of Curcumin on the surface of MWCNTs reduces its ability to cause inflammation, oxidative stress, and cell death. Graphical Abstract (a) Synthesis of Curcumin-coated-Lysine-functionalized MWCNTs. (b) Flow of research depicting experimental groups and studies performed along with the underlying techniques used.https://doi.org/10.1007/s10856-024-06789-9 |
| spellingShingle | Samiksha Rele Chanchal Kiran Thakur Fatima Khan Budhadev Baral Vaishali Saini Chandrabose Karthikeyan N. S. Hari Narayana Moorthy Hem Chandra Jha Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity Journal of Materials Science: Materials in Medicine |
| title | Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity |
| title_full | Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity |
| title_fullStr | Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity |
| title_full_unstemmed | Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity |
| title_short | Curcumin coating: a novel solution to mitigate inherent carbon nanotube toxicity |
| title_sort | curcumin coating a novel solution to mitigate inherent carbon nanotube toxicity |
| url | https://doi.org/10.1007/s10856-024-06789-9 |
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