Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine model
Abstract Hypervitaminosis D leads to toxic effects, including hypercalcemia, which can cause severe damage to various organs. Fetuin-A, a glycoprotein with anti-inflammatory properties, may protect tissues from such damage. This study explores the role of Fetuin-A in mitigating hypervitaminosis D-in...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-85200-1 |
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| author | Maha A. Mohamed Mohamed Hussein Shefaa Moustafa Yalda Rahmani Tooba Ahmed Durrani Shiza Ali Hafsa Ubaid Chhapra Elshimaa Ali Mariam Shadan |
| author_facet | Maha A. Mohamed Mohamed Hussein Shefaa Moustafa Yalda Rahmani Tooba Ahmed Durrani Shiza Ali Hafsa Ubaid Chhapra Elshimaa Ali Mariam Shadan |
| author_sort | Maha A. Mohamed |
| collection | DOAJ |
| description | Abstract Hypervitaminosis D leads to toxic effects, including hypercalcemia, which can cause severe damage to various organs. Fetuin-A, a glycoprotein with anti-inflammatory properties, may protect tissues from such damage. This study explores the role of Fetuin-A in mitigating hypervitaminosis D-induced damage in renal, hepatic, and cardiac tissues. The objectives of this study were to: (1) Assess the extent of tissue damage from high-dose vitamin D in a murine model by examining the histopathological changes in liver, kidney and heart. (2) Investigate Fetuin-A's protective effect against this damage. Thirty-six albino rats were divided into four groups: (1) control, (2) vitamin D toxicity, (3) Fetuin-A + vitamin D, and (4) Fetuin-A only. Vitamin D was administered subcutaneously at 250 μg/20 g/day for 3 days. Fetuin-A was given at 100 μl/20 g, starting 7 days before vitamin D treatment. Histopathological analysis of liver, kidney, and heart tissues was performed using H&E and Alizarin Red staining and findings were analysed statistically. Vitamin D toxicity caused significant tissue damage, including apoptosis, inflammation, and calcification in the liver, kidneys, and heart. Pre-treatment with Fetuin-A reduced calcification and inflammation, preserving tissue architecture. Fetuin-A-only rats showed no damage or calcification. Fetuin-A provided statistically significant protection against vitamin D-induced damage, reducing oxidative stress and calcification in affected organs. These findings suggest Fetuin-A could be a potential therapeutic agent for hypervitaminosis D. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-3f2a39fdd0b04380a84dee0d0b4f9dfe2025-01-12T12:15:45ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-85200-1Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine modelMaha A. Mohamed0Mohamed Hussein1Shefaa Moustafa2Yalda Rahmani3Tooba Ahmed Durrani4Shiza Ali5Hafsa Ubaid Chhapra6Elshimaa Ali7Mariam Shadan8Department of Biomedical Sciences, Dubai Medical College for GirlsDepartment of Biomedical Sciences, Dubai Medical College for GirlsDepartment of Biomedical Sciences, Dubai Medical College for GirlsDepartment of Biomedical Sciences, Dubai Medical College for GirlsDepartment of Biomedical Sciences, Dubai Medical College for GirlsDepartment of Biomedical Sciences, Dubai Medical College for GirlsDepartment of Biomedical Sciences, Dubai Medical College for GirlsDepartment of Biomedical Sciences, Dubai Medical College for GirlsDepartment of Biomedical Sciences, Dubai Medical College for GirlsAbstract Hypervitaminosis D leads to toxic effects, including hypercalcemia, which can cause severe damage to various organs. Fetuin-A, a glycoprotein with anti-inflammatory properties, may protect tissues from such damage. This study explores the role of Fetuin-A in mitigating hypervitaminosis D-induced damage in renal, hepatic, and cardiac tissues. The objectives of this study were to: (1) Assess the extent of tissue damage from high-dose vitamin D in a murine model by examining the histopathological changes in liver, kidney and heart. (2) Investigate Fetuin-A's protective effect against this damage. Thirty-six albino rats were divided into four groups: (1) control, (2) vitamin D toxicity, (3) Fetuin-A + vitamin D, and (4) Fetuin-A only. Vitamin D was administered subcutaneously at 250 μg/20 g/day for 3 days. Fetuin-A was given at 100 μl/20 g, starting 7 days before vitamin D treatment. Histopathological analysis of liver, kidney, and heart tissues was performed using H&E and Alizarin Red staining and findings were analysed statistically. Vitamin D toxicity caused significant tissue damage, including apoptosis, inflammation, and calcification in the liver, kidneys, and heart. Pre-treatment with Fetuin-A reduced calcification and inflammation, preserving tissue architecture. Fetuin-A-only rats showed no damage or calcification. Fetuin-A provided statistically significant protection against vitamin D-induced damage, reducing oxidative stress and calcification in affected organs. These findings suggest Fetuin-A could be a potential therapeutic agent for hypervitaminosis D.https://doi.org/10.1038/s41598-025-85200-1Hypervitaminosis DFetuin-ATissue damageCalcificationVitamin D toxicity |
| spellingShingle | Maha A. Mohamed Mohamed Hussein Shefaa Moustafa Yalda Rahmani Tooba Ahmed Durrani Shiza Ali Hafsa Ubaid Chhapra Elshimaa Ali Mariam Shadan Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine model Scientific Reports Hypervitaminosis D Fetuin-A Tissue damage Calcification Vitamin D toxicity |
| title | Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine model |
| title_full | Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine model |
| title_fullStr | Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine model |
| title_full_unstemmed | Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine model |
| title_short | Histopathological effects of hypervitaminosis-D and the protective role of fetuin-A in renal, hepatic, and cardiac tissues in a murine model |
| title_sort | histopathological effects of hypervitaminosis d and the protective role of fetuin a in renal hepatic and cardiac tissues in a murine model |
| topic | Hypervitaminosis D Fetuin-A Tissue damage Calcification Vitamin D toxicity |
| url | https://doi.org/10.1038/s41598-025-85200-1 |
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