Immunotargeting of Integrin αβ for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model
To explore suitable imaging probes for early and specific detection of pancreatic cancer, we demonstrated that α 6 β 4 integrin is a good target and employed single-photon emission computed tomography (SPECT) or near-infrared (NIR) imaging for immunotargeting. Expression levels of α 6 β 4 were exami...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2016-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012115624917 |
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| author | Winn Aung MBBS, PhD Atsushi B. Tsuji PhD Hitomi Sudo PhD Aya Sugyo M.Ed Takako Furukawa PhD Yoshinori Ukai PhD Yoshikazu Kurosawa PhD Tsuneo Saga MD, PhD |
| author_facet | Winn Aung MBBS, PhD Atsushi B. Tsuji PhD Hitomi Sudo PhD Aya Sugyo M.Ed Takako Furukawa PhD Yoshinori Ukai PhD Yoshikazu Kurosawa PhD Tsuneo Saga MD, PhD |
| author_sort | Winn Aung MBBS, PhD |
| collection | DOAJ |
| description | To explore suitable imaging probes for early and specific detection of pancreatic cancer, we demonstrated that α 6 β 4 integrin is a good target and employed single-photon emission computed tomography (SPECT) or near-infrared (NIR) imaging for immunotargeting. Expression levels of α 6 β 4 were examined by Western blotting and flow cytometry in certain human pancreatic cancer cell lines. The human cell line BxPC-3 was used for α 6 β 4 -positive and a mouse cell line, A4, was used for negative counterpart. We labeled antibody against α 6 β 4 with Indium-111 ( 111 In) or indocyanine green (ICG). After injection of 111 In-labeled probe to tumor-bearing mice, biodistribution, SPECT, autoradiography (ARG), and immunohistochemical (IHC) studies were conducted. After administration of ICG-labeled probe, in vivo and ex vivo NIR imaging and fluorescence microscopy of tumors were performed. BxPC-3 tumor showed a higher radioligand binding in SPECT and higher fluorescence intensity as well as a delay in the probe washout in NIR imaging when compared to A4 tumor. The biodistribution profile of 111 In-labeled probe, ARG, and IHC confirmed the α 6 β 4 specific binding of the probe. Here, we propose that α 6 β 4 is a desirable target for the diagnosis of pancreatic cancer and that it could be detected by radionuclide imaging and NIR imaging using a radiolabeled or ICG-labeled α 6 β 4 antibody. |
| format | Article |
| id | doaj-art-3f222c3e446e4dc4a57ee9b0d3c06972 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-3f222c3e446e4dc4a57ee9b0d3c069722025-01-03T00:11:32ZengSAGE PublishingMolecular Imaging1536-01212016-01-011510.1177/153601211562491710.1177_1536012115624917Immunotargeting of Integrin αβ for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer ModelWinn Aung MBBS, PhD0Atsushi B. Tsuji PhD1Hitomi Sudo PhD2Aya Sugyo M.Ed3Takako Furukawa PhD4Yoshinori Ukai PhD5Yoshikazu Kurosawa PhD6Tsuneo Saga MD, PhD7Diagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, JapanDiagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, JapanDiagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, JapanDiagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, JapanDiagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, JapanPerseus Proteomics Inc, Tokyo, JapanInnovation Center for Advanced Medicine, Fujita Health University, Toyoake, JapanDiagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, JapanTo explore suitable imaging probes for early and specific detection of pancreatic cancer, we demonstrated that α 6 β 4 integrin is a good target and employed single-photon emission computed tomography (SPECT) or near-infrared (NIR) imaging for immunotargeting. Expression levels of α 6 β 4 were examined by Western blotting and flow cytometry in certain human pancreatic cancer cell lines. The human cell line BxPC-3 was used for α 6 β 4 -positive and a mouse cell line, A4, was used for negative counterpart. We labeled antibody against α 6 β 4 with Indium-111 ( 111 In) or indocyanine green (ICG). After injection of 111 In-labeled probe to tumor-bearing mice, biodistribution, SPECT, autoradiography (ARG), and immunohistochemical (IHC) studies were conducted. After administration of ICG-labeled probe, in vivo and ex vivo NIR imaging and fluorescence microscopy of tumors were performed. BxPC-3 tumor showed a higher radioligand binding in SPECT and higher fluorescence intensity as well as a delay in the probe washout in NIR imaging when compared to A4 tumor. The biodistribution profile of 111 In-labeled probe, ARG, and IHC confirmed the α 6 β 4 specific binding of the probe. Here, we propose that α 6 β 4 is a desirable target for the diagnosis of pancreatic cancer and that it could be detected by radionuclide imaging and NIR imaging using a radiolabeled or ICG-labeled α 6 β 4 antibody.https://doi.org/10.1177/1536012115624917 |
| spellingShingle | Winn Aung MBBS, PhD Atsushi B. Tsuji PhD Hitomi Sudo PhD Aya Sugyo M.Ed Takako Furukawa PhD Yoshinori Ukai PhD Yoshikazu Kurosawa PhD Tsuneo Saga MD, PhD Immunotargeting of Integrin αβ for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model Molecular Imaging |
| title | Immunotargeting of Integrin αβ for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model |
| title_full | Immunotargeting of Integrin αβ for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model |
| title_fullStr | Immunotargeting of Integrin αβ for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model |
| title_full_unstemmed | Immunotargeting of Integrin αβ for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model |
| title_short | Immunotargeting of Integrin αβ for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model |
| title_sort | immunotargeting of integrin αβ for single photon emission computed tomography and near infrared fluorescence imaging in a pancreatic cancer model |
| url | https://doi.org/10.1177/1536012115624917 |
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