Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential
Endophytic fungi associated with selected aquatic plants, Eichhornia crassipes, Nymphaea nouchali, Salvinia minima and S. molesta were evaluated. Ectophoma salviniae sp. nov. and Neottiosporina mihintaleensis sp. nov. are introduced as novel taxa from Salvinia spp. from Sri Lanka. Chaetomella raphig...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| author | Madhara K. Wimalasena Madhara K. Wimalasena Madhara K. Wimalasena Nalin N. Wijayawardene Nalin N. Wijayawardene Nalin N. Wijayawardene Thushara C. Bamunuarachchige Gui-Qing Zhang R. G. Udeni Jayalal Darbhe J. Bhat Darbhe J. Bhat Turki M. Dawoud Heethaka K. S. de Zoysa Dong-Qin Dai |
| author_facet | Madhara K. Wimalasena Madhara K. Wimalasena Madhara K. Wimalasena Nalin N. Wijayawardene Nalin N. Wijayawardene Nalin N. Wijayawardene Thushara C. Bamunuarachchige Gui-Qing Zhang R. G. Udeni Jayalal Darbhe J. Bhat Darbhe J. Bhat Turki M. Dawoud Heethaka K. S. de Zoysa Dong-Qin Dai |
| author_sort | Madhara K. Wimalasena |
| collection | DOAJ |
| description | Endophytic fungi associated with selected aquatic plants, Eichhornia crassipes, Nymphaea nouchali, Salvinia minima and S. molesta were evaluated. Ectophoma salviniae sp. nov. and Neottiosporina mihintaleensis sp. nov. are introduced as novel taxa from Salvinia spp. from Sri Lanka. Chaetomella raphigera is reported as a new geographical record, Colletotrichum siamense and C. truncatum are reported as novel host records in aquatic plants, while Phyllosticta capitalensis has been identified on the same host (Nymphaea nouchali) in the North-Central Province of Sri Lanka. Identification of the fungi was based on morphological characteristics and multi-locus phylogenetic analyses using ITS, LSU, SSU, ACT, CHS-1, GAPDH, tub2, rpb2, and tef1-α molecular markers. The identified fungi were analysed for extracellular enzymatic properties. According to the qualitative analysis, Ectophoma salviniae sp. nov. exhibited the highest amylase production, Chaetomella raphigera exhibited the highest cellulase enzyme production, and Neottiosporina mihintaleensis sp. nov. exhibited the highest laccase production. The results demonstrate the aquatic fungal diversity in this region and their extracellular enzymatic potentials, providing valuable insights for future biotechnological approaches. |
| format | Article |
| id | doaj-art-3f1bd022bb1f49a0a0b8ee788901f79c |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-3f1bd022bb1f49a0a0b8ee788901f79c2025-01-08T06:11:53ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.14751141475114Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potentialMadhara K. Wimalasena0Madhara K. Wimalasena1Madhara K. Wimalasena2Nalin N. Wijayawardene3Nalin N. Wijayawardene4Nalin N. Wijayawardene5Thushara C. Bamunuarachchige6Gui-Qing Zhang7R. G. Udeni Jayalal8Darbhe J. Bhat9Darbhe J. Bhat10Turki M. Dawoud11Heethaka K. S. de Zoysa12Dong-Qin Dai13Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan, ChinaFaculty of Graduate Studies, Sabaragamuwa University of Sri Lanka, Belihuloya, Sri LankaDepartment of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka, Mihintale, Sri LankaCenter for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan, ChinaDepartment of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka, Mihintale, Sri LankaTropical Microbiology Research Foundation, Colombo, Sri LankaDepartment of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka, Mihintale, Sri LankaCenter for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan, ChinaDepartment of Natural Resources, Faculty of Applied Sciences, Sabaragamuwa University of Sri Lanka, Belihuloya, Sri LankaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaVishnugupta Vishwavidyapeetam, Gokarna, IndiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka, Mihintale, Sri LankaCenter for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan, ChinaEndophytic fungi associated with selected aquatic plants, Eichhornia crassipes, Nymphaea nouchali, Salvinia minima and S. molesta were evaluated. Ectophoma salviniae sp. nov. and Neottiosporina mihintaleensis sp. nov. are introduced as novel taxa from Salvinia spp. from Sri Lanka. Chaetomella raphigera is reported as a new geographical record, Colletotrichum siamense and C. truncatum are reported as novel host records in aquatic plants, while Phyllosticta capitalensis has been identified on the same host (Nymphaea nouchali) in the North-Central Province of Sri Lanka. Identification of the fungi was based on morphological characteristics and multi-locus phylogenetic analyses using ITS, LSU, SSU, ACT, CHS-1, GAPDH, tub2, rpb2, and tef1-α molecular markers. The identified fungi were analysed for extracellular enzymatic properties. According to the qualitative analysis, Ectophoma salviniae sp. nov. exhibited the highest amylase production, Chaetomella raphigera exhibited the highest cellulase enzyme production, and Neottiosporina mihintaleensis sp. nov. exhibited the highest laccase production. The results demonstrate the aquatic fungal diversity in this region and their extracellular enzymatic potentials, providing valuable insights for future biotechnological approaches.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1475114/fullfreshwater plantsfungal endophyteshydrolytic and oxidative enzymesnew speciesphylogenytaxonomy |
| spellingShingle | Madhara K. Wimalasena Madhara K. Wimalasena Madhara K. Wimalasena Nalin N. Wijayawardene Nalin N. Wijayawardene Nalin N. Wijayawardene Thushara C. Bamunuarachchige Gui-Qing Zhang R. G. Udeni Jayalal Darbhe J. Bhat Darbhe J. Bhat Turki M. Dawoud Heethaka K. S. de Zoysa Dong-Qin Dai Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential Frontiers in Cellular and Infection Microbiology freshwater plants fungal endophytes hydrolytic and oxidative enzymes new species phylogeny taxonomy |
| title | Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential |
| title_full | Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential |
| title_fullStr | Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential |
| title_full_unstemmed | Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential |
| title_short | Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential |
| title_sort | ectophoma salviniae sp nov neottiosporina mihintaleensis sp nov and four other endophytes associated with aquatic plants from sri lanka and their extracellular enzymatic potential |
| topic | freshwater plants fungal endophytes hydrolytic and oxidative enzymes new species phylogeny taxonomy |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1475114/full |
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