The assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition
Abstract The study addresses the utilization of food waste by-products from faba bean (Vicia faba L.) pods (FBP) as an alternative feed supplement to promote sustainable piglet growth by reducing antimicrobial use. Objectives include evaluation of FBP in terms of nutritional components (proximate co...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84925-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841544811513905152 |
|---|---|
| author | Inna Vlasova Philip Krüsselmann Yuliia Kostenko Maciej Obrębski Sebastian Granica Wilfried Vahjen Jürgen Zentek Marcin Równicki Jakub P. Piwowarski |
| author_facet | Inna Vlasova Philip Krüsselmann Yuliia Kostenko Maciej Obrębski Sebastian Granica Wilfried Vahjen Jürgen Zentek Marcin Równicki Jakub P. Piwowarski |
| author_sort | Inna Vlasova |
| collection | DOAJ |
| description | Abstract The study addresses the utilization of food waste by-products from faba bean (Vicia faba L.) pods (FBP) as an alternative feed supplement to promote sustainable piglet growth by reducing antimicrobial use. Objectives include evaluation of FBP in terms of nutritional components (proximate composition, fibres, minerals), phytochemical composition (total phenols, HPLC-MS profiling), and in vitro biological activities. Air-dried FBP from the cultivar ‘Bizon’ contained high levels of crude protein (144 g/kg), dietary fibre (413 g/kg), potassium (27.8 g/kg), and iron (126 mg/kg). Phytochemical analysis of methanolic extract from FBP revealed significant levels of polyphenols, including vestitol, piscidic acid, hydroxyeucomic acid, quercetin, and kaempferol glycosides with no detectable tannins. The extract showed negligible activity against porcine digestive enzymes (α-amylase, lipase, and trypsin) (IC50 > 4 mg/mL) and demonstrated a dose-dependent antibacterial activity against Escherichia coli and Salmonella enterica in concentrations of 1–8 mg/mL. The extract had low cytotoxicity (IC50 = 432.6 µg/mL) against IPEC-J2 – cells derived from porcine jejunal epithelium. The results indicate that FBP ‘Bizon’ is a valuable source of bioactive compounds with antibacterial properties, without adverse effects on porcine enzymes or IPEC-J2 cells, supporting its potential as a sustainable feed in piglet nutrition, in line with circular economy concepts. |
| format | Article |
| id | doaj-art-3f05639fbae6441ba34045b7de852d9f |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-3f05639fbae6441ba34045b7de852d9f2025-01-12T12:15:06ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-024-84925-9The assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutritionInna Vlasova0Philip Krüsselmann1Yuliia Kostenko2Maciej Obrębski3Sebastian Granica4Wilfried Vahjen5Jürgen Zentek6Marcin Równicki7Jakub P. Piwowarski8Microbiota Lab, Department of Pharmaceutical Biology, Medical University of WarsawInstitute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität BerlinMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of WarsawMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of WarsawMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of WarsawInstitute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität BerlinInstitute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität BerlinMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of WarsawMicrobiota Lab, Department of Pharmaceutical Biology, Medical University of WarsawAbstract The study addresses the utilization of food waste by-products from faba bean (Vicia faba L.) pods (FBP) as an alternative feed supplement to promote sustainable piglet growth by reducing antimicrobial use. Objectives include evaluation of FBP in terms of nutritional components (proximate composition, fibres, minerals), phytochemical composition (total phenols, HPLC-MS profiling), and in vitro biological activities. Air-dried FBP from the cultivar ‘Bizon’ contained high levels of crude protein (144 g/kg), dietary fibre (413 g/kg), potassium (27.8 g/kg), and iron (126 mg/kg). Phytochemical analysis of methanolic extract from FBP revealed significant levels of polyphenols, including vestitol, piscidic acid, hydroxyeucomic acid, quercetin, and kaempferol glycosides with no detectable tannins. The extract showed negligible activity against porcine digestive enzymes (α-amylase, lipase, and trypsin) (IC50 > 4 mg/mL) and demonstrated a dose-dependent antibacterial activity against Escherichia coli and Salmonella enterica in concentrations of 1–8 mg/mL. The extract had low cytotoxicity (IC50 = 432.6 µg/mL) against IPEC-J2 – cells derived from porcine jejunal epithelium. The results indicate that FBP ‘Bizon’ is a valuable source of bioactive compounds with antibacterial properties, without adverse effects on porcine enzymes or IPEC-J2 cells, supporting its potential as a sustainable feed in piglet nutrition, in line with circular economy concepts.https://doi.org/10.1038/s41598-024-84925-9Faba bean podsFood by-productPolyphenolsDigestive enzymesAntimicrobials |
| spellingShingle | Inna Vlasova Philip Krüsselmann Yuliia Kostenko Maciej Obrębski Sebastian Granica Wilfried Vahjen Jürgen Zentek Marcin Równicki Jakub P. Piwowarski The assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition Scientific Reports Faba bean pods Food by-product Polyphenols Digestive enzymes Antimicrobials |
| title | The assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition |
| title_full | The assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition |
| title_fullStr | The assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition |
| title_full_unstemmed | The assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition |
| title_short | The assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition |
| title_sort | assessment of chemical composition and biological activity of faba bean pods as a potential feed additive utilized in piglets nutrition |
| topic | Faba bean pods Food by-product Polyphenols Digestive enzymes Antimicrobials |
| url | https://doi.org/10.1038/s41598-024-84925-9 |
| work_keys_str_mv | AT innavlasova theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT philipkrusselmann theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT yuliiakostenko theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT maciejobrebski theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT sebastiangranica theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT wilfriedvahjen theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT jurgenzentek theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT marcinrownicki theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT jakubppiwowarski theassessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT innavlasova assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT philipkrusselmann assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT yuliiakostenko assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT maciejobrebski assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT sebastiangranica assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT wilfriedvahjen assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT jurgenzentek assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT marcinrownicki assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition AT jakubppiwowarski assessmentofchemicalcompositionandbiologicalactivityoffababeanpodsasapotentialfeedadditiveutilizedinpigletsnutrition |