The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937
IntroductionThe free exopolysaccharide (f-EPS) produced by Streptococcus thermophilus is a natural texture modifier and has a variety of prebiotic activities. Our previous studies showed f-EPS production from S. thermophilus 937 was increased 2-fold in the presence of 15 mM of glutamate, isoleucine,...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1476940/full |
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| author | Yunchao Wa Yunchao Wa Xia Zhao Xia Zhao Chenchen Zhang Chenchen Zhang Hengxian Qu Hengxian Qu Dawei Chen Dawei Chen Xia Chen Xia Chen Yujun Huang Yujun Huang Ruixia Gu Ruixia Gu |
| author_facet | Yunchao Wa Yunchao Wa Xia Zhao Xia Zhao Chenchen Zhang Chenchen Zhang Hengxian Qu Hengxian Qu Dawei Chen Dawei Chen Xia Chen Xia Chen Yujun Huang Yujun Huang Ruixia Gu Ruixia Gu |
| author_sort | Yunchao Wa |
| collection | DOAJ |
| description | IntroductionThe free exopolysaccharide (f-EPS) produced by Streptococcus thermophilus is a natural texture modifier and has a variety of prebiotic activities. Our previous studies showed f-EPS production from S. thermophilus 937 was increased 2-fold in the presence of 15 mM of glutamate, isoleucine, and histidine in the chemically defined medium.MethodsIn this study, we used transcriptomics and qPCR to further explore the specific mechanism of the enhanced effect of 3 amino acids on the f-EPS biosynthesis of S. thermophilus 937.ResultsThe mRNA-seq analysis and targeted pathway analysis indicated that genes associated with histidine/valine/leucine/ isoleucine/phenylalanine/tyrosine/tryptophan synthesis, galactose metabolism, purine metabolism and quorum sensing in S. thermophilus 937 were significantly upregulated under increasing concentrations of histidine, isoleucine and glutamate in chemically defined medium (CDM). qPCR results showed that the significant upregulation of galactose metabolism- and nucleotide sugar synthesis-related genes was attributed to the increase in glutamate concentration, and glutamate could induce the expression of galR. The upregulation of epsA, epsB, and epsD transcript levels was caused by the increase in histidine concentration. The upregulation of transcript levels of genes related to phenylalanine/tyrosine/tryptophan/histidine/ valine/leucine/isoleucine synthesis was caused by the increase in isoleucine concentrations.DiscussionThis indicates that 3 amino acids have different mechanisms for enhancing the biosynthesis of f-EPS in S. thermophilus 937. |
| format | Article |
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| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-3ee89dc89c9f45b3aa4ae593457736a92025-01-08T06:12:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14769401476940The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937Yunchao Wa0Yunchao Wa1Xia Zhao2Xia Zhao3Chenchen Zhang4Chenchen Zhang5Hengxian Qu6Hengxian Qu7Dawei Chen8Dawei Chen9Xia Chen10Xia Chen11Yujun Huang12Yujun Huang13Ruixia Gu14Ruixia Gu15Key Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, ChinaKey Lab of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, ChinaIntroductionThe free exopolysaccharide (f-EPS) produced by Streptococcus thermophilus is a natural texture modifier and has a variety of prebiotic activities. Our previous studies showed f-EPS production from S. thermophilus 937 was increased 2-fold in the presence of 15 mM of glutamate, isoleucine, and histidine in the chemically defined medium.MethodsIn this study, we used transcriptomics and qPCR to further explore the specific mechanism of the enhanced effect of 3 amino acids on the f-EPS biosynthesis of S. thermophilus 937.ResultsThe mRNA-seq analysis and targeted pathway analysis indicated that genes associated with histidine/valine/leucine/ isoleucine/phenylalanine/tyrosine/tryptophan synthesis, galactose metabolism, purine metabolism and quorum sensing in S. thermophilus 937 were significantly upregulated under increasing concentrations of histidine, isoleucine and glutamate in chemically defined medium (CDM). qPCR results showed that the significant upregulation of galactose metabolism- and nucleotide sugar synthesis-related genes was attributed to the increase in glutamate concentration, and glutamate could induce the expression of galR. The upregulation of epsA, epsB, and epsD transcript levels was caused by the increase in histidine concentration. The upregulation of transcript levels of genes related to phenylalanine/tyrosine/tryptophan/histidine/ valine/leucine/isoleucine synthesis was caused by the increase in isoleucine concentrations.DiscussionThis indicates that 3 amino acids have different mechanisms for enhancing the biosynthesis of f-EPS in S. thermophilus 937.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1476940/fullStreptococcus thermophilusamino acidexopolysaccharidetranscriptomicsquantitative PCR |
| spellingShingle | Yunchao Wa Yunchao Wa Xia Zhao Xia Zhao Chenchen Zhang Chenchen Zhang Hengxian Qu Hengxian Qu Dawei Chen Dawei Chen Xia Chen Xia Chen Yujun Huang Yujun Huang Ruixia Gu Ruixia Gu The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937 Frontiers in Microbiology Streptococcus thermophilus amino acid exopolysaccharide transcriptomics quantitative PCR |
| title | The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937 |
| title_full | The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937 |
| title_fullStr | The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937 |
| title_full_unstemmed | The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937 |
| title_short | The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937 |
| title_sort | transcriptional regulation effects of histidine isoleucine and glutamate on free exopolysaccharide biosynthesis of streptococcus thermophilus 937 |
| topic | Streptococcus thermophilus amino acid exopolysaccharide transcriptomics quantitative PCR |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1476940/full |
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