Evaluation of Antiproliferative Potentials Associated with the Volatile Compounds of <i>Lantana camara</i> Flowers: Selective In Vitro Activity

Probing the chemical profiles and biological activities of medicinal plants is important for the discovery of new potent therapeutic products. Our study deciphers the chemical composition of the essential oils (EOs) obtained from three different flowers of <i>Lantana camara</i> and evalu...

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Main Authors: Jennifer El Hajj, Louna Karam, Ali Jaber, Edmond Cheble, Elias Akoury, Philippe Hussein Kobeissy, José-Noel Ibrahim, Ali Yassin
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/29/22/5431
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author Jennifer El Hajj
Louna Karam
Ali Jaber
Edmond Cheble
Elias Akoury
Philippe Hussein Kobeissy
José-Noel Ibrahim
Ali Yassin
author_facet Jennifer El Hajj
Louna Karam
Ali Jaber
Edmond Cheble
Elias Akoury
Philippe Hussein Kobeissy
José-Noel Ibrahim
Ali Yassin
author_sort Jennifer El Hajj
collection DOAJ
description Probing the chemical profiles and biological activities of medicinal plants is important for the discovery of new potent therapeutic products. Our study deciphers the chemical composition of the essential oils (EOs) obtained from three different flowers of <i>Lantana camara</i> and evaluates their antioxidant and anticancer activities. This work represents the first study of EOs obtained from this plant and is based particularly on the difference in flower color. In addition, no other reports dealing specifically with the antitumor effects of such flower-derived EOs have been described in the literature. The collected flowers, white, pink, and orange, were extracted by hydrodistillation to yield EO1, EO2, and EO3 respectively. Gas chromatography–mass spectroscopy was primarily employed to identify the existing volatile compounds in the samples. Their antioxidant activities were screened through both DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging assays and FRAP (ferric-reducing antioxidant power) assays. The antiproliferative effects were evaluated on two distinct breast cancer cell lines, MCF-7 and MDA-MB-231, and compared to a normal human breast cell line, MCF-10A, using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) assay. All EOs showed notable antioxidant potential attributed to the active phytochemical compounds, with results being supported by a positive correlation between such activity and the total phenolic and flavonoid content. The most eminent, EO1, revealed a selective dose-dependent antiproliferative effect in both breast cancer cell lines, thus reflecting its potent role as an anticancer agent. We suggest that this highly selective activity is associated with the presence of bicyclogermacrene and <i>epi</i>-bicyclosesquiphellandrene in its chemical composition.
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spelling doaj-art-3ed2f84c44f04e438d2e9be8a3a93b1b2024-11-26T18:15:59ZengMDPI AGMolecules1420-30492024-11-012922543110.3390/molecules29225431Evaluation of Antiproliferative Potentials Associated with the Volatile Compounds of <i>Lantana camara</i> Flowers: Selective In Vitro ActivityJennifer El Hajj0Louna Karam1Ali Jaber2Edmond Cheble3Elias Akoury4Philippe Hussein Kobeissy5José-Noel Ibrahim6Ali Yassin7RDMPN Laboratory, Faculty of Pharmacy, Lebanese University, Beirut BP 14/6573, LebanonDepartment of Biological Sciences, School of Arts and Sciences, Lebanese American University, Beirut 1102-2801, LebanonDepartment of Physical Sciences, School of Arts and Sciences, Lebanese American University, Beirut 1102-2801, LebanonRDMPN Laboratory, Faculty of Pharmacy, Lebanese University, Beirut BP 14/6573, LebanonDepartment of Physical Sciences, School of Arts and Sciences, Lebanese American University, Beirut 1102-2801, LebanonDepartment of Biological Sciences, School of Arts and Sciences, Lebanese American University, Beirut 1102-2801, LebanonDepartment of Biological Sciences, School of Arts and Sciences, Lebanese American University, Beirut 1102-2801, LebanonDepartment of Physical Sciences, School of Arts and Sciences, Lebanese American University, Beirut 1102-2801, LebanonProbing the chemical profiles and biological activities of medicinal plants is important for the discovery of new potent therapeutic products. Our study deciphers the chemical composition of the essential oils (EOs) obtained from three different flowers of <i>Lantana camara</i> and evaluates their antioxidant and anticancer activities. This work represents the first study of EOs obtained from this plant and is based particularly on the difference in flower color. In addition, no other reports dealing specifically with the antitumor effects of such flower-derived EOs have been described in the literature. The collected flowers, white, pink, and orange, were extracted by hydrodistillation to yield EO1, EO2, and EO3 respectively. Gas chromatography–mass spectroscopy was primarily employed to identify the existing volatile compounds in the samples. Their antioxidant activities were screened through both DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging assays and FRAP (ferric-reducing antioxidant power) assays. The antiproliferative effects were evaluated on two distinct breast cancer cell lines, MCF-7 and MDA-MB-231, and compared to a normal human breast cell line, MCF-10A, using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) assay. All EOs showed notable antioxidant potential attributed to the active phytochemical compounds, with results being supported by a positive correlation between such activity and the total phenolic and flavonoid content. The most eminent, EO1, revealed a selective dose-dependent antiproliferative effect in both breast cancer cell lines, thus reflecting its potent role as an anticancer agent. We suggest that this highly selective activity is associated with the presence of bicyclogermacrene and <i>epi</i>-bicyclosesquiphellandrene in its chemical composition.https://www.mdpi.com/1420-3049/29/22/5431bicyclogermacrene<i>epi</i>-bicyclosesquiphellandreneanticancer activity<i>Lantana camara</i>essential oilchemical composition
spellingShingle Jennifer El Hajj
Louna Karam
Ali Jaber
Edmond Cheble
Elias Akoury
Philippe Hussein Kobeissy
José-Noel Ibrahim
Ali Yassin
Evaluation of Antiproliferative Potentials Associated with the Volatile Compounds of <i>Lantana camara</i> Flowers: Selective In Vitro Activity
Molecules
bicyclogermacrene
<i>epi</i>-bicyclosesquiphellandrene
anticancer activity
<i>Lantana camara</i>
essential oil
chemical composition
title Evaluation of Antiproliferative Potentials Associated with the Volatile Compounds of <i>Lantana camara</i> Flowers: Selective In Vitro Activity
title_full Evaluation of Antiproliferative Potentials Associated with the Volatile Compounds of <i>Lantana camara</i> Flowers: Selective In Vitro Activity
title_fullStr Evaluation of Antiproliferative Potentials Associated with the Volatile Compounds of <i>Lantana camara</i> Flowers: Selective In Vitro Activity
title_full_unstemmed Evaluation of Antiproliferative Potentials Associated with the Volatile Compounds of <i>Lantana camara</i> Flowers: Selective In Vitro Activity
title_short Evaluation of Antiproliferative Potentials Associated with the Volatile Compounds of <i>Lantana camara</i> Flowers: Selective In Vitro Activity
title_sort evaluation of antiproliferative potentials associated with the volatile compounds of i lantana camara i flowers selective in vitro activity
topic bicyclogermacrene
<i>epi</i>-bicyclosesquiphellandrene
anticancer activity
<i>Lantana camara</i>
essential oil
chemical composition
url https://www.mdpi.com/1420-3049/29/22/5431
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