Prevalence of vaginal and cervical HPV infection among 35-year age cohort ever-married women in Kalutara district of Sri Lanka and the validity of vaginal HPV/DNA specimen as a cervical cancer screening tool: a cross-sectional study
Abstract Background Cervical cancer is the 2nd most common female cancer among Sri Lankan females and is almost associated with sexually transmitted cervicovaginal human papillomavirus (HPV) infection. The study objectives were to determine the prevalence of vaginal and cervical HPV infection among...
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2024-11-01
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| Online Access: | https://doi.org/10.1186/s12879-024-10150-4 |
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| author | KCM Perera KN Mapitigama HTCS Abeysena |
| author_facet | KCM Perera KN Mapitigama HTCS Abeysena |
| author_sort | KCM Perera |
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| description | Abstract Background Cervical cancer is the 2nd most common female cancer among Sri Lankan females and is almost associated with sexually transmitted cervicovaginal human papillomavirus (HPV) infection. The study objectives were to determine the prevalence of vaginal and cervical HPV infection among 35year old ever-married women and assess the validity of primary healthcare provider-collected vaginal HPV/DNA specimens as a cervical cancer screening tool to improve the coverage of the programme. Method A descriptive cross-sectional study was carried out from the 1st of September 2018 to the 31st of January 2019. Ever-married women 35 years of age in Kalutara district were the study population. Two women from each Public Health Midwife area (n = 413) were selected randomly from the relevant area eligible families register/s. HPV/DNA cervical specimen and vaginal specimen collection (n = 621) were carried out. Specimens were screened by the Cobas 4800 HPV/DNA automated Polymerase Chain Reaction (PCR) machine. Participants’ profiles were recorded by the research assistants using an interviewer-administered questionnaire. Results The overall prevalence of vaginal and cervical HPV infection was 7.08% (95% CI; 5.2-9.4%) and 6.12% (95% CI; 4.26-8.3%) respectively. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), diagnostic accuracy, and the kappa coefficient of the vaginal HPV/DNA screening method vs. cervical HPV/DNA screening method were 100%, 98.9%, 86.4%, 100%, 99% and 0.92 respectively. Conclusions Vaginal HPV/DNA specimen screening method can be used as a cervical cancer screening tool due to its high validity. Pilots of the feasibility should be set up before the regional or national rollout of vaginal sampling strategies. |
| format | Article |
| id | doaj-art-3e98412c595f47d38e82d8bf8eb1b025 |
| institution | Kabale University |
| issn | 1471-2334 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | BMC Infectious Diseases |
| spelling | doaj-art-3e98412c595f47d38e82d8bf8eb1b0252024-11-10T12:10:14ZengBMCBMC Infectious Diseases1471-23342024-11-012411710.1186/s12879-024-10150-4Prevalence of vaginal and cervical HPV infection among 35-year age cohort ever-married women in Kalutara district of Sri Lanka and the validity of vaginal HPV/DNA specimen as a cervical cancer screening tool: a cross-sectional studyKCM Perera0KN Mapitigama1HTCS Abeysena2Consultant Community Physician, Nutrition Division, Ministry of HealthConsultant Community Physician, Family Health BureauSenior Professor in Community Medicine, University of KelaniyaAbstract Background Cervical cancer is the 2nd most common female cancer among Sri Lankan females and is almost associated with sexually transmitted cervicovaginal human papillomavirus (HPV) infection. The study objectives were to determine the prevalence of vaginal and cervical HPV infection among 35year old ever-married women and assess the validity of primary healthcare provider-collected vaginal HPV/DNA specimens as a cervical cancer screening tool to improve the coverage of the programme. Method A descriptive cross-sectional study was carried out from the 1st of September 2018 to the 31st of January 2019. Ever-married women 35 years of age in Kalutara district were the study population. Two women from each Public Health Midwife area (n = 413) were selected randomly from the relevant area eligible families register/s. HPV/DNA cervical specimen and vaginal specimen collection (n = 621) were carried out. Specimens were screened by the Cobas 4800 HPV/DNA automated Polymerase Chain Reaction (PCR) machine. Participants’ profiles were recorded by the research assistants using an interviewer-administered questionnaire. Results The overall prevalence of vaginal and cervical HPV infection was 7.08% (95% CI; 5.2-9.4%) and 6.12% (95% CI; 4.26-8.3%) respectively. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), diagnostic accuracy, and the kappa coefficient of the vaginal HPV/DNA screening method vs. cervical HPV/DNA screening method were 100%, 98.9%, 86.4%, 100%, 99% and 0.92 respectively. Conclusions Vaginal HPV/DNA specimen screening method can be used as a cervical cancer screening tool due to its high validity. Pilots of the feasibility should be set up before the regional or national rollout of vaginal sampling strategies.https://doi.org/10.1186/s12879-024-10150-4PrevalenceHPV infectionCervicalVaginalHPV/DNA testVaginal HPV/DNA specimens |
| spellingShingle | KCM Perera KN Mapitigama HTCS Abeysena Prevalence of vaginal and cervical HPV infection among 35-year age cohort ever-married women in Kalutara district of Sri Lanka and the validity of vaginal HPV/DNA specimen as a cervical cancer screening tool: a cross-sectional study BMC Infectious Diseases Prevalence HPV infection Cervical Vaginal HPV/DNA test Vaginal HPV/DNA specimens |
| title | Prevalence of vaginal and cervical HPV infection among 35-year age cohort ever-married women in Kalutara district of Sri Lanka and the validity of vaginal HPV/DNA specimen as a cervical cancer screening tool: a cross-sectional study |
| title_full | Prevalence of vaginal and cervical HPV infection among 35-year age cohort ever-married women in Kalutara district of Sri Lanka and the validity of vaginal HPV/DNA specimen as a cervical cancer screening tool: a cross-sectional study |
| title_fullStr | Prevalence of vaginal and cervical HPV infection among 35-year age cohort ever-married women in Kalutara district of Sri Lanka and the validity of vaginal HPV/DNA specimen as a cervical cancer screening tool: a cross-sectional study |
| title_full_unstemmed | Prevalence of vaginal and cervical HPV infection among 35-year age cohort ever-married women in Kalutara district of Sri Lanka and the validity of vaginal HPV/DNA specimen as a cervical cancer screening tool: a cross-sectional study |
| title_short | Prevalence of vaginal and cervical HPV infection among 35-year age cohort ever-married women in Kalutara district of Sri Lanka and the validity of vaginal HPV/DNA specimen as a cervical cancer screening tool: a cross-sectional study |
| title_sort | prevalence of vaginal and cervical hpv infection among 35 year age cohort ever married women in kalutara district of sri lanka and the validity of vaginal hpv dna specimen as a cervical cancer screening tool a cross sectional study |
| topic | Prevalence HPV infection Cervical Vaginal HPV/DNA test Vaginal HPV/DNA specimens |
| url | https://doi.org/10.1186/s12879-024-10150-4 |
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