Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system
Genome-wide CRISPR library screening technology is a gene function research tool developed based on the CRISPR/Cas9 gene-editing system. The clustered regularly interspaced short palindromic repeats/CRISPR-associated genes (CRISPR/Cas) system, considered the third generation of gene editing after zi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1498641/full |
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| author | Shijie Hu Shijie Hu Mailin Gan Mailin Gan Ziang Wei Ziang Wei Pan Shang Pan Shang Lei Song Lei Song Jinkang Feng Jinkang Feng Lei Chen Lei Chen Lili Niu Lili Niu Yan Wang Yan Wang Yan Wang Shunhua Zhang Shunhua Zhang Linyuan Shen Linyuan Shen Linyuan Shen Li Zhu Li Zhu Li Zhu Ye Zhao Ye Zhao Ye Zhao |
| author_facet | Shijie Hu Shijie Hu Mailin Gan Mailin Gan Ziang Wei Ziang Wei Pan Shang Pan Shang Lei Song Lei Song Jinkang Feng Jinkang Feng Lei Chen Lei Chen Lili Niu Lili Niu Yan Wang Yan Wang Yan Wang Shunhua Zhang Shunhua Zhang Linyuan Shen Linyuan Shen Linyuan Shen Li Zhu Li Zhu Li Zhu Ye Zhao Ye Zhao Ye Zhao |
| author_sort | Shijie Hu |
| collection | DOAJ |
| description | Genome-wide CRISPR library screening technology is a gene function research tool developed based on the CRISPR/Cas9 gene-editing system. The clustered regularly interspaced short palindromic repeats/CRISPR-associated genes (CRISPR/Cas) system, considered the third generation of gene editing after zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), is widely used for screening various viral host factors. CRISPR libraries are classified into three main categories based on the different functions of Cas9 enzymes: CRISPR knockout (CRISPR KO) library screening, CRISPR transcriptional activation (CRISPRa) library screening, and CRISPR transcriptional interference (CRISPRi) library screening. Recently, genome-wide CRISPR library screening technology has been used to identify host factors that interact with viruses at various stages, including adsorption, endocytosis, and replication. By specifically modulating the expression of these host factors, it becomes possible to cultivate disease-resistant varieties, establish disease models, and design and develop vaccines, among other applications. This review provides an overview of the development and technical processes of genome-wide CRISPR library screening, as well as its applications in identifying viral host factors in livestock and poultry. |
| format | Article |
| id | doaj-art-3e48ac83267d43eaa42b502334d19e95 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-3e48ac83267d43eaa42b502334d19e952024-11-21T16:19:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-11-011510.3389/fmicb.2024.14986411498641Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR systemShijie Hu0Shijie Hu1Mailin Gan2Mailin Gan3Ziang Wei4Ziang Wei5Pan Shang6Pan Shang7Lei Song8Lei Song9Jinkang Feng10Jinkang Feng11Lei Chen12Lei Chen13Lili Niu14Lili Niu15Yan Wang16Yan Wang17Yan Wang18Shunhua Zhang19Shunhua Zhang20Linyuan Shen21Linyuan Shen22Linyuan Shen23Li Zhu24Li Zhu25Li Zhu26Ye Zhao27Ye Zhao28Ye Zhao29Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Ya’an, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan, Sichuan Agricultural University, Ya’an, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Ya’an, ChinaKey Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Ya’an, ChinaGenome-wide CRISPR library screening technology is a gene function research tool developed based on the CRISPR/Cas9 gene-editing system. The clustered regularly interspaced short palindromic repeats/CRISPR-associated genes (CRISPR/Cas) system, considered the third generation of gene editing after zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), is widely used for screening various viral host factors. CRISPR libraries are classified into three main categories based on the different functions of Cas9 enzymes: CRISPR knockout (CRISPR KO) library screening, CRISPR transcriptional activation (CRISPRa) library screening, and CRISPR transcriptional interference (CRISPRi) library screening. Recently, genome-wide CRISPR library screening technology has been used to identify host factors that interact with viruses at various stages, including adsorption, endocytosis, and replication. By specifically modulating the expression of these host factors, it becomes possible to cultivate disease-resistant varieties, establish disease models, and design and develop vaccines, among other applications. This review provides an overview of the development and technical processes of genome-wide CRISPR library screening, as well as its applications in identifying viral host factors in livestock and poultry.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1498641/fullCRISPR/Cas9library screeninggene editingviral host factorsantiviral |
| spellingShingle | Shijie Hu Shijie Hu Mailin Gan Mailin Gan Ziang Wei Ziang Wei Pan Shang Pan Shang Lei Song Lei Song Jinkang Feng Jinkang Feng Lei Chen Lei Chen Lili Niu Lili Niu Yan Wang Yan Wang Yan Wang Shunhua Zhang Shunhua Zhang Linyuan Shen Linyuan Shen Linyuan Shen Li Zhu Li Zhu Li Zhu Ye Zhao Ye Zhao Ye Zhao Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system Frontiers in Microbiology CRISPR/Cas9 library screening gene editing viral host factors antiviral |
| title | Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system |
| title_full | Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system |
| title_fullStr | Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system |
| title_full_unstemmed | Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system |
| title_short | Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system |
| title_sort | identification of host factors for livestock and poultry viruses genome wide screening technology based on the crispr system |
| topic | CRISPR/Cas9 library screening gene editing viral host factors antiviral |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1498641/full |
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