Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structures
The maintenance of healthy gut microbiota is of utmost significance when it comes to combating gastrointestinal disorders like diarrhoea. This study was designed to unveil the gut bacteriome signature and diversity by analyzing five diarrhoeic (DF) and five non-diarrhoeic feces (NDF) samples obtaine...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | The Microbe |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194625000196 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849343635106037760 |
|---|---|
| author | Zebunnahar Yasmin Mohammad Alamgir Hossain Sharmin Chowdhury Md. Habib Ullah Masum Md. Shaminur Rahman M. Nazmul Hoque AMAM. Zonaed Siddiki |
| author_facet | Zebunnahar Yasmin Mohammad Alamgir Hossain Sharmin Chowdhury Md. Habib Ullah Masum Md. Shaminur Rahman M. Nazmul Hoque AMAM. Zonaed Siddiki |
| author_sort | Zebunnahar Yasmin |
| collection | DOAJ |
| description | The maintenance of healthy gut microbiota is of utmost significance when it comes to combating gastrointestinal disorders like diarrhoea. This study was designed to unveil the gut bacteriome signature and diversity by analyzing five diarrhoeic (DF) and five non-diarrhoeic feces (NDF) samples obtained from 10 individual calves through 16S rRNA (V4 region) gene-based amplicon sequencing. Our findings revealed substantial taxonomic variability between DF and NDF (p = 0.0127), indicated by their higher degree of shared microbiota. Among the identified genera, Gallibacterium and Veillonella were the major bacterial genera detected in the gut of calves, with marked discrepancies in their relative abundances in DF and NDF. We successfully identified 44 genera, including Sedimentibacter, Lonepinella, Sulfurospirillum, Haemophilus, and Enterobacter. which are linked to calf diarrhoea. The DF and NDF included 264 distinct bacterial species, of which 43.56 % were shared between sample categories, and 38.63 % and 17.80 % were found solely in DF and NDF, respectively. Moreover, Gallibacterium salpingitidis (43.37 %) was the most prevalent species in DF samples, followed by Gallibacterium anatis (17.56 %). In contrast, Veillonella magna (19.21 %) had the highest prevalence in NDF samples, followed by Bacteroides sp. (18.00 %). The findings suggested that diarrhoea affects the gut bacteriome in calves, with evidence for the existence of both unique and shared bacteriomes with pathogenic implications in the gut of calves. We expect that upcoming innovations will pave the way for personalized, microbiota-centered therapies, presenting hopeful approaches to influence microbial populations for better health results effectively. |
| format | Article |
| id | doaj-art-3e42a9f4b06d48a1921f2e9c61f5fc58 |
| institution | Kabale University |
| issn | 2950-1946 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-3e42a9f4b06d48a1921f2e9c61f5fc582025-08-20T03:42:55ZengElsevierThe Microbe2950-19462025-03-01610025110.1016/j.microb.2025.100251Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structuresZebunnahar Yasmin0Mohammad Alamgir Hossain1Sharmin Chowdhury2Md. Habib Ullah Masum3Md. Shaminur Rahman4M. Nazmul Hoque5AMAM. Zonaed Siddiki6Genomics Research Group, Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University (CVASU), Khulshi 4225, BangladeshGenomics Research Group, Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University (CVASU), Khulshi 4225, BangladeshGenomics Research Group, Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University (CVASU), Khulshi 4225, BangladeshDepartment of Genomics and Bioinformatics, Faculty of Biotechnology and Genetic Engineering, Chattogram Veterinary and Animal Sciences University (CVASU), Khulshi 4225, BangladeshDepartment of Microbiology, Jashore University of Science and Technology, Jashore, Jashore 7408, BangladeshMolecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur 1706, BangladeshGenomics Research Group, Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University (CVASU), Khulshi 4225, Bangladesh; Nextgen Informatics Ltd, Bangladesh; Corresponding author at: Genomics Research Group, Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University (CVASU), Khulshi 4225, Bangladesh.The maintenance of healthy gut microbiota is of utmost significance when it comes to combating gastrointestinal disorders like diarrhoea. This study was designed to unveil the gut bacteriome signature and diversity by analyzing five diarrhoeic (DF) and five non-diarrhoeic feces (NDF) samples obtained from 10 individual calves through 16S rRNA (V4 region) gene-based amplicon sequencing. Our findings revealed substantial taxonomic variability between DF and NDF (p = 0.0127), indicated by their higher degree of shared microbiota. Among the identified genera, Gallibacterium and Veillonella were the major bacterial genera detected in the gut of calves, with marked discrepancies in their relative abundances in DF and NDF. We successfully identified 44 genera, including Sedimentibacter, Lonepinella, Sulfurospirillum, Haemophilus, and Enterobacter. which are linked to calf diarrhoea. The DF and NDF included 264 distinct bacterial species, of which 43.56 % were shared between sample categories, and 38.63 % and 17.80 % were found solely in DF and NDF, respectively. Moreover, Gallibacterium salpingitidis (43.37 %) was the most prevalent species in DF samples, followed by Gallibacterium anatis (17.56 %). In contrast, Veillonella magna (19.21 %) had the highest prevalence in NDF samples, followed by Bacteroides sp. (18.00 %). The findings suggested that diarrhoea affects the gut bacteriome in calves, with evidence for the existence of both unique and shared bacteriomes with pathogenic implications in the gut of calves. We expect that upcoming innovations will pave the way for personalized, microbiota-centered therapies, presenting hopeful approaches to influence microbial populations for better health results effectively.http://www.sciencedirect.com/science/article/pii/S2950194625000196DiarrhoeaCalvesGut bacteriome16S rRNA amplicon sequencing |
| spellingShingle | Zebunnahar Yasmin Mohammad Alamgir Hossain Sharmin Chowdhury Md. Habib Ullah Masum Md. Shaminur Rahman M. Nazmul Hoque AMAM. Zonaed Siddiki Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structures The Microbe Diarrhoea Calves Gut bacteriome 16S rRNA amplicon sequencing |
| title | Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structures |
| title_full | Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structures |
| title_fullStr | Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structures |
| title_full_unstemmed | Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structures |
| title_short | Comparison of fecal bacteriome of diarrhoeic and non-diarrhoeic calves revealed diversified community structures |
| title_sort | comparison of fecal bacteriome of diarrhoeic and non diarrhoeic calves revealed diversified community structures |
| topic | Diarrhoea Calves Gut bacteriome 16S rRNA amplicon sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2950194625000196 |
| work_keys_str_mv | AT zebunnaharyasmin comparisonoffecalbacteriomeofdiarrhoeicandnondiarrhoeiccalvesrevealeddiversifiedcommunitystructures AT mohammadalamgirhossain comparisonoffecalbacteriomeofdiarrhoeicandnondiarrhoeiccalvesrevealeddiversifiedcommunitystructures AT sharminchowdhury comparisonoffecalbacteriomeofdiarrhoeicandnondiarrhoeiccalvesrevealeddiversifiedcommunitystructures AT mdhabibullahmasum comparisonoffecalbacteriomeofdiarrhoeicandnondiarrhoeiccalvesrevealeddiversifiedcommunitystructures AT mdshaminurrahman comparisonoffecalbacteriomeofdiarrhoeicandnondiarrhoeiccalvesrevealeddiversifiedcommunitystructures AT mnazmulhoque comparisonoffecalbacteriomeofdiarrhoeicandnondiarrhoeiccalvesrevealeddiversifiedcommunitystructures AT amamzonaedsiddiki comparisonoffecalbacteriomeofdiarrhoeicandnondiarrhoeiccalvesrevealeddiversifiedcommunitystructures |