Enhancing Potato Virus X Diagnosis: Chelex ™ 100 Resin RNA Extraction Method Evaluation
Potato Virus X (PVX) is a prevalent pathogen affecting potato crops, leading to substantial yield losses. This study investigates the efficacy of Chelex™ 100 resin RNA extraction method for PVX detection, through RT-qPCR analysis. We evaluate RNA yield, sensitivity, and detection limits of this met...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
"Alexandru Ioan Cuza" University of Iași
2024-11-01
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| Series: | Journal of Experimental and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/202 |
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| Summary: | Potato Virus X (PVX) is a prevalent pathogen affecting potato crops, leading to substantial yield losses. This study investigates the efficacy of Chelex™ 100 resin RNA extraction method for PVX detection, through RT-qPCR analysis. We evaluate RNA yield, sensitivity, and detection limits of this method compared with Phenol-chloroform RNA extraction protocol. Our results demonstrate that Chelex™ 100 resin extraction yields superior RNA concentrations and exhibits greater sensitivity compared to ELISA immunoassay diagnostic test. RT-qPCR successfully detects PVX RNA in dilutions up to 1:100 using Chelex™ 100 resin extraction. These findings highlight the potential of Chelex™ 100 resin extraction coupled with RT-qPCR as a rapid and reliable method for PVX detection.
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| ISSN: | 2601-6974 |