Anatomical Insights into Cerebral and Craniofacial Morphology in ASD Using Anthropometric Measurements
Background: The association of neurodevelopmental and craniofacial anomalies with Autism Spectrum Disorder (ASD) is indicative of cerebral abnormalities. To examine cerebral and craniofacial anatomical variations in a rodent model of ASD using defined anthropometric measures. Methods: This in v...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
ziauddin University
2025-07-01
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| Series: | Pakistan Journal of Medicine and Dentistry |
| Subjects: | |
| Online Access: | https://ojs.zu.edu.pk/pjmd/article/view/3796 |
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| Summary: | Background: The association of neurodevelopmental and craniofacial anomalies with Autism Spectrum Disorder (ASD) is indicative of cerebral abnormalities. To examine cerebral and craniofacial anatomical variations in a rodent model of ASD using defined anthropometric measures.
Methods: This in vivo study was performed in an animal research facility and analysis was done (Ref#18/05/223) at Liaquat University of Medical & Health Sciences (LUMHS), Jamshoro and Continental Medical College, Lahore (CMC) from June to December 2023. Twenty Albino Wistar rats were allocated into two groups. Control (n=6) and ASD-imposed (n = 14) under the administration of propionic acid (PPA) using a random sampling technique. OpenEpi 3.0.0 was used for sample size calculation. The craniofacial and cerebral markers were measured using the calipers and stereotactic referencing after euthanasia. The skull length, intercanthal width, head circumference, and cranial index were considered. H&E staining was also performed to view histological patterns in certain brain tissues. Statistical analysis was done using SPSS version 26. Independent t-tests and Chi-square tests were performed. A p-value of <0.05 was considered statistically significant.
Results: The ASD-induced group exhibited significantly reduced skull length (29.3 ± 2.1 mm vs. 32.8 ± 1.5 mm; p = 0.002) and head circumference (40.2 ± 2.7 mm vs. 42.5 ± 2.2 mm; p = 0.041), alongside increased intercanthal distance (7.8 ± 0.6 mm vs. 6.2 ± 0.4 mm; p = 0.0001) and cranial index (81.9% vs. 75.3%; p = 0.003). Histologically, cortical layering was disrupted in 10 of 14 ASD rats, and ventricular enlargement was observed in 12 of 14, compared to none in controls (all p < 0.01).
Conclusion: Craniofacial morphometric studies of ASD rodent models show quantifiable anatomical differences associated with neurodevelopmental compromising. This strategy can act as a translational model for grasping structural markers of early ASD detection and pathogenesis.
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| ISSN: | 2313-7371 2308-2593 |