Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia
Islet transplantation in diabetes is hampered by the need of life-long immunosuppression. Encapsulation provides partial immunoprotection but could possibly limit oxygen supply, a factor that may enhance hypoxia-induced beta cell death in the early posttransplantation period. Here we tested suscepti...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2013/374925 |
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| author | I. K. Hals A. M. Rokstad B. L. Strand J. Oberholzer V. Grill |
| author_facet | I. K. Hals A. M. Rokstad B. L. Strand J. Oberholzer V. Grill |
| author_sort | I. K. Hals |
| collection | DOAJ |
| description | Islet transplantation in diabetes is hampered by the need of life-long immunosuppression. Encapsulation provides partial immunoprotection but could possibly limit oxygen supply, a factor that may enhance hypoxia-induced beta cell death in the early posttransplantation period. Here we tested susceptibility of alginate microencapsulated human islets to experimental hypoxia (0.1–0.3% O2 for 8 h, followed by reoxygenation) on viability and functional parameters. Hypoxia reduced viability as measured by MTT by 33.8±3.5% in encapsulated and 42.9±5.2% in nonencapsulated islets (P<0.2). Nonencapsulated islets released 37.7% (median) more HMGB1 compared to encapsulated islets after hypoxic culture conditions (P<0.001). Glucose-induced insulin release was marginally affected by hypoxia. Basal oxygen consumption was equally reduced in encapsulated and nonencapsulated islets, by 22.0±6.1% versus 24.8±5.7%. Among 27 tested cytokines/chemokines, hypoxia increased the secretion of IL-6 and IL-8/CXCL8 in both groups of islets, whereas an increase of MCP-1/CCL2 was seen only with nonencapsulated islets. Conclusion. Alginate microencapsulation of human islets does not increase susceptibility to acute hypoxia. This is a positive finding in relation to potential use of encapsulation for islet transplantation. |
| format | Article |
| id | doaj-art-3dd24b03a5a149f9a650af9dd621d9d9 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-3dd24b03a5a149f9a650af9dd621d9d92025-02-03T05:52:59ZengWileyJournal of Diabetes Research2314-67452314-67532013-01-01201310.1155/2013/374925374925Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute HypoxiaI. K. Hals0A. M. Rokstad1B. L. Strand2J. Oberholzer3V. Grill4Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Postbox 8905, 7491 Trondheim, NorwayDepartment of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Postbox 8905, 7491 Trondheim, NorwayDepartment of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Postbox 8905, 7491 Trondheim, NorwayDepartment of Surgery, University of Illinois, IL at Chicago, Chicago, IL 60612, USADepartment of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Postbox 8905, 7491 Trondheim, NorwayIslet transplantation in diabetes is hampered by the need of life-long immunosuppression. Encapsulation provides partial immunoprotection but could possibly limit oxygen supply, a factor that may enhance hypoxia-induced beta cell death in the early posttransplantation period. Here we tested susceptibility of alginate microencapsulated human islets to experimental hypoxia (0.1–0.3% O2 for 8 h, followed by reoxygenation) on viability and functional parameters. Hypoxia reduced viability as measured by MTT by 33.8±3.5% in encapsulated and 42.9±5.2% in nonencapsulated islets (P<0.2). Nonencapsulated islets released 37.7% (median) more HMGB1 compared to encapsulated islets after hypoxic culture conditions (P<0.001). Glucose-induced insulin release was marginally affected by hypoxia. Basal oxygen consumption was equally reduced in encapsulated and nonencapsulated islets, by 22.0±6.1% versus 24.8±5.7%. Among 27 tested cytokines/chemokines, hypoxia increased the secretion of IL-6 and IL-8/CXCL8 in both groups of islets, whereas an increase of MCP-1/CCL2 was seen only with nonencapsulated islets. Conclusion. Alginate microencapsulation of human islets does not increase susceptibility to acute hypoxia. This is a positive finding in relation to potential use of encapsulation for islet transplantation.http://dx.doi.org/10.1155/2013/374925 |
| spellingShingle | I. K. Hals A. M. Rokstad B. L. Strand J. Oberholzer V. Grill Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia Journal of Diabetes Research |
| title | Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia |
| title_full | Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia |
| title_fullStr | Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia |
| title_full_unstemmed | Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia |
| title_short | Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia |
| title_sort | alginate microencapsulation of human islets does not increase susceptibility to acute hypoxia |
| url | http://dx.doi.org/10.1155/2013/374925 |
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