Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3
IntroductionTransmissible gastroenteritis virus (TGEV), as the causative agent of TGE, causes huge economic losses in the pig industry worldwide.MethodsIn this study, we successfully constructed a reverse genetic system for the TGEV TH-98 strain and rescued the recombinant virus rTH-98-Δ3-COE-HA bas...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1609022/full |
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| author | Zhenye Hao Zhenye Hao Lifei Liu Lifei Liu Shuai Wang Shuai Wang Yanping Jiang Yanping Jiang Jiaxuan Li Jiaxuan Li Wen Cui Wen Cui Li Wang Li Wang Yijing Li Yijing Li |
| author_facet | Zhenye Hao Zhenye Hao Lifei Liu Lifei Liu Shuai Wang Shuai Wang Yanping Jiang Yanping Jiang Jiaxuan Li Jiaxuan Li Wen Cui Wen Cui Li Wang Li Wang Yijing Li Yijing Li |
| author_sort | Zhenye Hao |
| collection | DOAJ |
| description | IntroductionTransmissible gastroenteritis virus (TGEV), as the causative agent of TGE, causes huge economic losses in the pig industry worldwide.MethodsIn this study, we successfully constructed a reverse genetic system for the TGEV TH-98 strain and rescued the recombinant virus rTH-98-Δ3-COE-HA based on this system.ResultsThe results showed that ORF3 is a non-structural protein and does not exist in mature virions. Therefore, rTH-98-Δ3-COE-HA induced inflammatory cytokine expression in IPEC-J2 cells, but the expression levels were significantly lower than those triggered by TH-98 and rTH-98, indicating that TGEV ORF3 may play a critical role in inducing the host immune response. To detect whether the exogenous protein expressed in the rTH-98-Δ3-COE-HA has immunogenicity, the results showed that the levels of antibodies were significantly increased in mice. rTH-98-Δ3-COE-HA can induce a specific immune response in the host body against its expressed exogenous proteins.Discussion and conclusionOur study provides important clues for revealing the role of ORF3 in virus replication and pathogenesis, laying a theoretical and material foundation for the construction of recombinant viral multi-valent vaccines. |
| format | Article |
| id | doaj-art-3daa1fb8d3cc4f8dbe45c35b692a2d2e |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-3daa1fb8d3cc4f8dbe45c35b692a2d2e2025-08-20T03:46:12ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-06-011510.3389/fcimb.2025.16090221609022Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3Zhenye Hao0Zhenye Hao1Lifei Liu2Lifei Liu3Shuai Wang4Shuai Wang5Yanping Jiang6Yanping Jiang7Jiaxuan Li8Jiaxuan Li9Wen Cui10Wen Cui11Li Wang12Li Wang13Yijing Li14Yijing Li15College of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, ChinaIntroductionTransmissible gastroenteritis virus (TGEV), as the causative agent of TGE, causes huge economic losses in the pig industry worldwide.MethodsIn this study, we successfully constructed a reverse genetic system for the TGEV TH-98 strain and rescued the recombinant virus rTH-98-Δ3-COE-HA based on this system.ResultsThe results showed that ORF3 is a non-structural protein and does not exist in mature virions. Therefore, rTH-98-Δ3-COE-HA induced inflammatory cytokine expression in IPEC-J2 cells, but the expression levels were significantly lower than those triggered by TH-98 and rTH-98, indicating that TGEV ORF3 may play a critical role in inducing the host immune response. To detect whether the exogenous protein expressed in the rTH-98-Δ3-COE-HA has immunogenicity, the results showed that the levels of antibodies were significantly increased in mice. rTH-98-Δ3-COE-HA can induce a specific immune response in the host body against its expressed exogenous proteins.Discussion and conclusionOur study provides important clues for revealing the role of ORF3 in virus replication and pathogenesis, laying a theoretical and material foundation for the construction of recombinant viral multi-valent vaccines.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1609022/fullTGEVORF3reverse geneticsviral replicationimmunogenicity |
| spellingShingle | Zhenye Hao Zhenye Hao Lifei Liu Lifei Liu Shuai Wang Shuai Wang Yanping Jiang Yanping Jiang Jiaxuan Li Jiaxuan Li Wen Cui Wen Cui Li Wang Li Wang Yijing Li Yijing Li Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3 Frontiers in Cellular and Infection Microbiology TGEV ORF3 reverse genetics viral replication immunogenicity |
| title | Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3 |
| title_full | Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3 |
| title_fullStr | Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3 |
| title_full_unstemmed | Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3 |
| title_short | Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3 |
| title_sort | establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3 |
| topic | TGEV ORF3 reverse genetics viral replication immunogenicity |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1609022/full |
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