Pathogenic differentiation and biological control of Verticillium wilt of eggplant
Verticillium wilt of eggplant occurs seriously in some areas of Zhejiang Province. To control the disease effectively, the disease observation and pathogenicity tests of pathogen were carried out, and the pathotype and physiological races of pathogen were identified. Furthermore, inhibitory activity...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2019-08-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.11.262 |
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| Summary: | Verticillium wilt of eggplant occurs seriously in some areas of Zhejiang Province. To control the disease effectively, the disease observation and pathogenicity tests of pathogen were carried out, and the pathotype and physiological races of pathogen were identified. Furthermore, inhibitory activity of Paenibacillus polymyxa strains against hypha growth and conidial germination of pathogen were determined, and inoculation assays for controlling Verticillium wilt of eggplant and plant growth promotion were conducted, and volatile metabolites and lipopeptides produced by P. polymyxa strains were analyzed. Results of disease observation and pathogenicity tests indicated that Verticillium wilt of eggplant in greenhouse occurred usually in mid-January, and it was caused by Verticillium dahliae Kleb. Identification results of pathotype and physiological races of pathogen with the representative strains demonstrated that seven isolates from Zhejiang Province all belonged to the defoliation pathotype and physiological race-2, respectively. In vitro experiments showed that the strain ShX301 had a highest inhibitory activity against hypha growth and conidial germination of pathogens isolated the diseased eggplant plants among five P. polymyxa strains. The in vivo experiments for controlling Verticillium wilt of eggplant and plant growth promotion displayed that the strain ShX301 had a highest inhibitory activity. Inoculation by the strain ShX301 reduced disease incidence and severity by 30% and 20.02%, respectively, and promoted eggplant plant growth with enhancing aboveground seedling stem length and biomass by 59.24% and 69.41%, as well as underground biomass by 54.11%, respectively. The determination of volatile metabolites showed that the five strains of P. polymyxa were able to produce volatile metabolites, which inhibited hypha growth of pathogen, but there were no significant differences among the strains. Matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis revealed that the fusaricidins were main activity compounds of antagonistic pathogen. Therefore, this study provides a scientific theory basis for development of the strain ShX301 as a biocontrol or plant growth promotion agent. |
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| ISSN: | 1008-9209 2097-5155 |