Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source
Carotenoids are pigments attracting the attention of several industries due to their antioxidant, biological and coloring properties. Low-cost substrates, such as agro-industrial wastes, are being investigated as a viable option to reduce microbial production costs in processes in which microorganis...
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| Language: | English |
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Elsevier
2024-01-01
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| Series: | Current Research in Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590262824000911 |
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| author | Micaela Giani Carmen Pire Rosa María Martínez-Espinosa |
| author_facet | Micaela Giani Carmen Pire Rosa María Martínez-Espinosa |
| author_sort | Micaela Giani |
| collection | DOAJ |
| description | Carotenoids are pigments attracting the attention of several industries due to their antioxidant, biological and coloring properties. Low-cost substrates, such as agro-industrial wastes, are being investigated as a viable option to reduce microbial production costs in processes in which microorganisms such as haloarchaea are used as cell factories to produce marketed compounds like carotenoids. They can grow on various agro-industrial wastes and produce the C50 carotenoid bacterioruberin (BR), which is an extraordinary antioxidant compound with anticancer properties. In this study, the haloarchaeon Haloferax mediterranei is grown in the presence of starch residues from the candy industry to induce the production of carotenoids. Cells grew successfully with this industrial waste (max. O.D. 600 nm = 27.75 ± 0.09). Biomass production increased in the presence of higher quantities of starch up to 17.3 ± 0.2 mg/ml of cell culture. The maximum BR concentration was 97.39 ± 1.86 µg/ml. The total amount of BRs synthesized increased when cells grew with increasing concentrations of the industrial starch. The relative percentages of all-trans-BR, 5-cis-BR and a double isomeric BR rose, whereas 9-cis-BR and 13-cis-BR levels decreased.Herein, haloarchaeal growth and carotenoid production can be enhanced using industrial waste products as the starch residues selected for this experiment which were provided by a candy company. |
| format | Article |
| id | doaj-art-3d65c59a2b6a4f6f8c8c171e88a2b6c9 |
| institution | Kabale University |
| issn | 2590-2628 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Biotechnology |
| spelling | doaj-art-3d65c59a2b6a4f6f8c8c171e88a2b6c92024-12-04T05:13:48ZengElsevierCurrent Research in Biotechnology2590-26282024-01-018100265Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon sourceMicaela Giani0Carmen Pire1Rosa María Martínez-Espinosa2Biochemistry and Molecular Biology and Edaphology and Agricultural Chemistry Department, Faculty of Sciences, University of Alicante, Ap. 99, E-03080 Alicante, Spain; Multidisciplinary Institute for Environmental Studies “Ramón Margalef” University of Alicante, Ap. 99, E-03080 Alicante, SpainBiochemistry and Molecular Biology and Edaphology and Agricultural Chemistry Department, Faculty of Sciences, University of Alicante, Ap. 99, E-03080 Alicante, Spain; Multidisciplinary Institute for Environmental Studies “Ramón Margalef” University of Alicante, Ap. 99, E-03080 Alicante, SpainBiochemistry and Molecular Biology and Edaphology and Agricultural Chemistry Department, Faculty of Sciences, University of Alicante, Ap. 99, E-03080 Alicante, Spain; Multidisciplinary Institute for Environmental Studies “Ramón Margalef” University of Alicante, Ap. 99, E-03080 Alicante, Spain; Corresponding author at: Biochemistry and Molecular Biology and Edaphology and Agricultural Chemistry Department, Faculty of Sciences, University of Alicante, Ap. 99, E-03080 Alicante, Spain.Carotenoids are pigments attracting the attention of several industries due to their antioxidant, biological and coloring properties. Low-cost substrates, such as agro-industrial wastes, are being investigated as a viable option to reduce microbial production costs in processes in which microorganisms such as haloarchaea are used as cell factories to produce marketed compounds like carotenoids. They can grow on various agro-industrial wastes and produce the C50 carotenoid bacterioruberin (BR), which is an extraordinary antioxidant compound with anticancer properties. In this study, the haloarchaeon Haloferax mediterranei is grown in the presence of starch residues from the candy industry to induce the production of carotenoids. Cells grew successfully with this industrial waste (max. O.D. 600 nm = 27.75 ± 0.09). Biomass production increased in the presence of higher quantities of starch up to 17.3 ± 0.2 mg/ml of cell culture. The maximum BR concentration was 97.39 ± 1.86 µg/ml. The total amount of BRs synthesized increased when cells grew with increasing concentrations of the industrial starch. The relative percentages of all-trans-BR, 5-cis-BR and a double isomeric BR rose, whereas 9-cis-BR and 13-cis-BR levels decreased.Herein, haloarchaeal growth and carotenoid production can be enhanced using industrial waste products as the starch residues selected for this experiment which were provided by a candy company.http://www.sciencedirect.com/science/article/pii/S2590262824000911Haloferax mediterraneiBacterioruberinHaloarchaeaCarotenoidsCircular economyStarch |
| spellingShingle | Micaela Giani Carmen Pire Rosa María Martínez-Espinosa Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source Current Research in Biotechnology Haloferax mediterranei Bacterioruberin Haloarchaea Carotenoids Circular economy Starch |
| title | Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source |
| title_full | Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source |
| title_fullStr | Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source |
| title_full_unstemmed | Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source |
| title_short | Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source |
| title_sort | carotenoid production by haloferax mediterranei using starch residues from the candy industry as a carbon source |
| topic | Haloferax mediterranei Bacterioruberin Haloarchaea Carotenoids Circular economy Starch |
| url | http://www.sciencedirect.com/science/article/pii/S2590262824000911 |
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