Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in Iran
The loa22 protein is highly conserved among pathogenic Leptospira serovars and it is expressed during both acute and chronic infections. The aim of this study was to clone and sequence of the loa22 protein-encoding gene of Leptospira serovars. In this study, 23 pathogenic Leptospira serovars and two...
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Wiley
2024-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/jotm/3900663 |
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| author | Yeganeh Malek Mohammadi Pejvak Khaki Mehdi Gharakhani |
| author_facet | Yeganeh Malek Mohammadi Pejvak Khaki Mehdi Gharakhani |
| author_sort | Yeganeh Malek Mohammadi |
| collection | DOAJ |
| description | The loa22 protein is highly conserved among pathogenic Leptospira serovars and it is expressed during both acute and chronic infections. The aim of this study was to clone and sequence of the loa22 protein-encoding gene of Leptospira serovars. In this study, 23 pathogenic Leptospira serovars and two nonpathogenic Leptospira serovars were used. These serovars were obtained from the microbial culture collection of Leptospira Reference Laboratory, Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, Iran. Three serovars, including L. Sejroe Hardjo-bovis, L. Grippotyphosa, L. Canicola, are used in the preparation of the trivalent vaccine. The loa22 gene was amplified by specific primers and the PCR products were then purified using kit and were cloned into a pTZ57R/T vector and transformed in competent E. coli DH5α cells. The cells were then plated onto LB agar containing ampicillin and recombinant colonies subjected to colony PCR to confirm the presence of the Leptospiral gene. Positive colonies plasmid vector was isolated from cells by High Pure Plasmid Isolation Kit. The loa22 gene was detected in all 23 pathogenic serovars, while this gene was not observed in nonpathogenic L. biflexa. It was determined that the similarity percentage of the sequenced pathogenic serovars is between 95.5% and 100%. The results concluded that the loa22 gene was highly conserved among various pathogenic Leptospira serovars and can be used to develop an effective recombinant vaccine. |
| format | Article |
| id | doaj-art-3d51fe314d9546629a3bb863499e43ee |
| institution | Kabale University |
| issn | 1687-9694 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
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| series | Journal of Tropical Medicine |
| spelling | doaj-art-3d51fe314d9546629a3bb863499e43ee2025-01-05T00:00:01ZengWileyJournal of Tropical Medicine1687-96942024-01-01202410.1155/jotm/3900663Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in IranYeganeh Malek Mohammadi0Pejvak Khaki1Mehdi Gharakhani2Department of MicrobiologyDepartment of MicrobiologyDepartment of MicrobiologyThe loa22 protein is highly conserved among pathogenic Leptospira serovars and it is expressed during both acute and chronic infections. The aim of this study was to clone and sequence of the loa22 protein-encoding gene of Leptospira serovars. In this study, 23 pathogenic Leptospira serovars and two nonpathogenic Leptospira serovars were used. These serovars were obtained from the microbial culture collection of Leptospira Reference Laboratory, Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, Iran. Three serovars, including L. Sejroe Hardjo-bovis, L. Grippotyphosa, L. Canicola, are used in the preparation of the trivalent vaccine. The loa22 gene was amplified by specific primers and the PCR products were then purified using kit and were cloned into a pTZ57R/T vector and transformed in competent E. coli DH5α cells. The cells were then plated onto LB agar containing ampicillin and recombinant colonies subjected to colony PCR to confirm the presence of the Leptospiral gene. Positive colonies plasmid vector was isolated from cells by High Pure Plasmid Isolation Kit. The loa22 gene was detected in all 23 pathogenic serovars, while this gene was not observed in nonpathogenic L. biflexa. It was determined that the similarity percentage of the sequenced pathogenic serovars is between 95.5% and 100%. The results concluded that the loa22 gene was highly conserved among various pathogenic Leptospira serovars and can be used to develop an effective recombinant vaccine.http://dx.doi.org/10.1155/jotm/3900663 |
| spellingShingle | Yeganeh Malek Mohammadi Pejvak Khaki Mehdi Gharakhani Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in Iran Journal of Tropical Medicine |
| title | Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in Iran |
| title_full | Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in Iran |
| title_fullStr | Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in Iran |
| title_full_unstemmed | Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in Iran |
| title_short | Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in Iran |
| title_sort | molecular characterization of gene encoding outer membrane protein loa22 in pathogenic leptospira serovars in iran |
| url | http://dx.doi.org/10.1155/jotm/3900663 |
| work_keys_str_mv | AT yeganehmalekmohammadi molecularcharacterizationofgeneencodingoutermembraneproteinloa22inpathogenicleptospiraserovarsiniran AT pejvakkhaki molecularcharacterizationofgeneencodingoutermembraneproteinloa22inpathogenicleptospiraserovarsiniran AT mehdigharakhani molecularcharacterizationofgeneencodingoutermembraneproteinloa22inpathogenicleptospiraserovarsiniran |