A phytotherapeutic approach to hinder the resistance against clindamycin by MRSA: in vitro and in silico studies
Aims This study investigates the potential effects of essential oils (EOs) in enhancing the efficacy of clindamycin against Methicillin-resistant Staphylococcus aureus (MRSA) using in vitro and computer simulations. The research seeks to identify essential oils that exhibit synergistic activity with...
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Taylor & Francis Group
2025-12-01
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| Series: | Future Science OA |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20565623.2025.2458438 |
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| author | Amal Mayyas Ali Al-Samydai Nehaya Al-Karablieh Waleed A Zalloum Deniz Al-Tawalbeh Farah Al-Mamoori Rula A. Amr Hamdi Al Nsairat Simone Carradori Lidia Kamal Al-Halaseh Talal Aburjai |
| author_facet | Amal Mayyas Ali Al-Samydai Nehaya Al-Karablieh Waleed A Zalloum Deniz Al-Tawalbeh Farah Al-Mamoori Rula A. Amr Hamdi Al Nsairat Simone Carradori Lidia Kamal Al-Halaseh Talal Aburjai |
| author_sort | Amal Mayyas |
| collection | DOAJ |
| description | Aims This study investigates the potential effects of essential oils (EOs) in enhancing the efficacy of clindamycin against Methicillin-resistant Staphylococcus aureus (MRSA) using in vitro and computer simulations. The research seeks to identify essential oils that exhibit synergistic activity with clindamycin and determine their potential key active components.Materials and Methods Essential oils commonly used in traditional medicine were tested for their antimicrobial activity against MRSA. The minimum inhibitory concentration (MIC) was determined using in vitro microdilution assays. A synergistic test with clindamycin was performed, and molecular docking studies evaluated the interaction between a key compound (trans-cinnamaldehyde) and MRSA protein.Results EOs from Cinnamomum verum, Rosmarinus officinalis, Salvia officinalis, and Thymus vulgaris demonstrated significant inhibitory and synergistic activities against MRSA, standard strain, and human clinical isolates. Gas Chromatography/Mass Spectroscopy identified trans-cinnamaldehyde, eucalyptol, and thymol as prominent antibacterial compounds. Molecular docking studies confirmed trans-cinnamaldehyde’s strong binding to MRSA’s AgrA protein, elucidating its enhanced efficacy.Conclusion The study underscores the potential of plant-based therapies to augment the effectiveness of conventional antibiotics like clindamycin in combating MRSA and addressing antibiotic resistance by integrating traditional plant remedies with modern medical approaches. |
| format | Article |
| id | doaj-art-3d4c635bb2404ba5a8be4c54c9110e8a |
| institution | Kabale University |
| issn | 2056-5623 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Future Science OA |
| spelling | doaj-art-3d4c635bb2404ba5a8be4c54c9110e8a2025-02-03T07:18:32ZengTaylor & Francis GroupFuture Science OA2056-56232025-12-0111110.1080/20565623.2025.2458438A phytotherapeutic approach to hinder the resistance against clindamycin by MRSA: in vitro and in silico studiesAmal Mayyas0Ali Al-Samydai1Nehaya Al-Karablieh2Waleed A Zalloum3Deniz Al-Tawalbeh4Farah Al-Mamoori5Rula A. Amr6Hamdi Al Nsairat7Simone Carradori8Lidia Kamal Al-Halaseh9Talal Aburjai10Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, Madaba, JordanPharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, JordanDepartment of Plant Protection, School of Agriculture, The University of Jordan, Amman, JordanDepartment of Pharmacy, Faculty of Health Sciences, American University of Madaba, Madaba, JordanDepartment of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Irbid, JordanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Zarqa University, Zarqa, JordanDepartment of Pharmacy, Faculty of Health Sciences, American University of Madaba, Madaba, JordanPharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, JordanDepartment of Pharmacy “G. d’Annunzio”, University of Chieti-Pescara, Chieti, SC, ItalyDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Mutah University, Al-Karak, JordanFaculty of Pharmacy, The University of Jordan, Amman, JordanAims This study investigates the potential effects of essential oils (EOs) in enhancing the efficacy of clindamycin against Methicillin-resistant Staphylococcus aureus (MRSA) using in vitro and computer simulations. The research seeks to identify essential oils that exhibit synergistic activity with clindamycin and determine their potential key active components.Materials and Methods Essential oils commonly used in traditional medicine were tested for their antimicrobial activity against MRSA. The minimum inhibitory concentration (MIC) was determined using in vitro microdilution assays. A synergistic test with clindamycin was performed, and molecular docking studies evaluated the interaction between a key compound (trans-cinnamaldehyde) and MRSA protein.Results EOs from Cinnamomum verum, Rosmarinus officinalis, Salvia officinalis, and Thymus vulgaris demonstrated significant inhibitory and synergistic activities against MRSA, standard strain, and human clinical isolates. Gas Chromatography/Mass Spectroscopy identified trans-cinnamaldehyde, eucalyptol, and thymol as prominent antibacterial compounds. Molecular docking studies confirmed trans-cinnamaldehyde’s strong binding to MRSA’s AgrA protein, elucidating its enhanced efficacy.Conclusion The study underscores the potential of plant-based therapies to augment the effectiveness of conventional antibiotics like clindamycin in combating MRSA and addressing antibiotic resistance by integrating traditional plant remedies with modern medical approaches.https://www.tandfonline.com/doi/10.1080/20565623.2025.2458438Antibiotic resistanceessential oilsMRSAphytotherapyquorum sensingAgrA |
| spellingShingle | Amal Mayyas Ali Al-Samydai Nehaya Al-Karablieh Waleed A Zalloum Deniz Al-Tawalbeh Farah Al-Mamoori Rula A. Amr Hamdi Al Nsairat Simone Carradori Lidia Kamal Al-Halaseh Talal Aburjai A phytotherapeutic approach to hinder the resistance against clindamycin by MRSA: in vitro and in silico studies Future Science OA Antibiotic resistance essential oils MRSA phytotherapy quorum sensing AgrA |
| title | A phytotherapeutic approach to hinder the resistance against clindamycin by MRSA: in vitro and in silico studies |
| title_full | A phytotherapeutic approach to hinder the resistance against clindamycin by MRSA: in vitro and in silico studies |
| title_fullStr | A phytotherapeutic approach to hinder the resistance against clindamycin by MRSA: in vitro and in silico studies |
| title_full_unstemmed | A phytotherapeutic approach to hinder the resistance against clindamycin by MRSA: in vitro and in silico studies |
| title_short | A phytotherapeutic approach to hinder the resistance against clindamycin by MRSA: in vitro and in silico studies |
| title_sort | phytotherapeutic approach to hinder the resistance against clindamycin by mrsa in vitro and in silico studies |
| topic | Antibiotic resistance essential oils MRSA phytotherapy quorum sensing AgrA |
| url | https://www.tandfonline.com/doi/10.1080/20565623.2025.2458438 |
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