Intelligent model and optimization of ultrasound-assisted extraction of antioxidants and amylase enzyme from Gnaphalium affine D. Don

The study uses ultrasound-assisted extraction to recovery the antioxidant and amylase enzyme from Gnaphalium affine D. Don, namely “chewcut” in Thailand. The study involves two statistical methods: artificial neural networks (ANN) and response surface methodology (RSM) to model and optimize extracti...

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Main Authors: Naphatrapi Luangsakul, Kannika Kunyanee, Sandra Kusumawardani, Tai Van Ngo
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Ultrasonics Sonochemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1350417724004115
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author Naphatrapi Luangsakul
Kannika Kunyanee
Sandra Kusumawardani
Tai Van Ngo
author_facet Naphatrapi Luangsakul
Kannika Kunyanee
Sandra Kusumawardani
Tai Van Ngo
author_sort Naphatrapi Luangsakul
collection DOAJ
description The study uses ultrasound-assisted extraction to recovery the antioxidant and amylase enzyme from Gnaphalium affine D. Don, namely “chewcut” in Thailand. The study involves two statistical methods: artificial neural networks (ANN) and response surface methodology (RSM) to model and optimize extraction procedure for improving the yield of antioxidant and amylase enzyme activity (AEA). Both RSM and ANN showed the potential to predict and find the optimal extraction conditions. However, ANN model could give more accurate values compared with validation test. ANN model found that under optimal conditions (temperature: 65.92 °C, ultrasonic power: 58.22 %, extraction time: 37.95 min), the total phenolic compounds, total flavonoid compounds, antioxidant activity and AEA were 218.35 ± 0.34 mgGAE/g, 0.554 ± 0.045 mgQE/g, 84.2 ± 0.2 %, 364.14 ± 1.35 mg-maltose/g. This is the first report on amylase potential of chewcut, which could be further served as the natural enzyme source. Moreover, by adding its bioactive compounds, it may be possible to improve nutraceutical properties and quality of products.
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institution Kabale University
issn 1350-4177
language English
publishDate 2025-01-01
publisher Elsevier
record_format Article
series Ultrasonics Sonochemistry
spelling doaj-art-3d4558ce908f4b69aa0ef33e294de3fe2025-01-11T06:38:41ZengElsevierUltrasonics Sonochemistry1350-41772025-01-01112107162Intelligent model and optimization of ultrasound-assisted extraction of antioxidants and amylase enzyme from Gnaphalium affine D. DonNaphatrapi Luangsakul0Kannika Kunyanee1Sandra Kusumawardani2Tai Van Ngo3School of Food Industry, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, ThailandSchool of Food Industry, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, ThailandSchool of Food Industry, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, ThailandCorresponding author.; School of Food Industry, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, ThailandThe study uses ultrasound-assisted extraction to recovery the antioxidant and amylase enzyme from Gnaphalium affine D. Don, namely “chewcut” in Thailand. The study involves two statistical methods: artificial neural networks (ANN) and response surface methodology (RSM) to model and optimize extraction procedure for improving the yield of antioxidant and amylase enzyme activity (AEA). Both RSM and ANN showed the potential to predict and find the optimal extraction conditions. However, ANN model could give more accurate values compared with validation test. ANN model found that under optimal conditions (temperature: 65.92 °C, ultrasonic power: 58.22 %, extraction time: 37.95 min), the total phenolic compounds, total flavonoid compounds, antioxidant activity and AEA were 218.35 ± 0.34 mgGAE/g, 0.554 ± 0.045 mgQE/g, 84.2 ± 0.2 %, 364.14 ± 1.35 mg-maltose/g. This is the first report on amylase potential of chewcut, which could be further served as the natural enzyme source. Moreover, by adding its bioactive compounds, it may be possible to improve nutraceutical properties and quality of products.http://www.sciencedirect.com/science/article/pii/S1350417724004115Gnaphalium affine D. DonAmylaseAntioxidantOptimizationModelling
spellingShingle Naphatrapi Luangsakul
Kannika Kunyanee
Sandra Kusumawardani
Tai Van Ngo
Intelligent model and optimization of ultrasound-assisted extraction of antioxidants and amylase enzyme from Gnaphalium affine D. Don
Ultrasonics Sonochemistry
Gnaphalium affine D. Don
Amylase
Antioxidant
Optimization
Modelling
title Intelligent model and optimization of ultrasound-assisted extraction of antioxidants and amylase enzyme from Gnaphalium affine D. Don
title_full Intelligent model and optimization of ultrasound-assisted extraction of antioxidants and amylase enzyme from Gnaphalium affine D. Don
title_fullStr Intelligent model and optimization of ultrasound-assisted extraction of antioxidants and amylase enzyme from Gnaphalium affine D. Don
title_full_unstemmed Intelligent model and optimization of ultrasound-assisted extraction of antioxidants and amylase enzyme from Gnaphalium affine D. Don
title_short Intelligent model and optimization of ultrasound-assisted extraction of antioxidants and amylase enzyme from Gnaphalium affine D. Don
title_sort intelligent model and optimization of ultrasound assisted extraction of antioxidants and amylase enzyme from gnaphalium affine d don
topic Gnaphalium affine D. Don
Amylase
Antioxidant
Optimization
Modelling
url http://www.sciencedirect.com/science/article/pii/S1350417724004115
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AT sandrakusumawardani intelligentmodelandoptimizationofultrasoundassistedextractionofantioxidantsandamylaseenzymefromgnaphaliumaffineddon
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