Abnormal expression of miR-668-3p in non-small cell lung cancer patients and its correlation with serum-related tumor markers
Abstract Background The accuracy and reliability of identified biomarkers in differentiating early non-small cell lung cancer (NSCLC) remain suboptimal, thereby impeding the timely detection of NSCLC.The objective of this research is to examine the expression level and diagnostic utility of miR-668-...
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BMC
2025-01-01
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| Series: | Journal of Cardiothoracic Surgery |
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| Online Access: | https://doi.org/10.1186/s13019-024-03220-w |
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| author | Wen Yang Ling Liu Jianliang Li Yingchao Liu Lin Rong Junjie Ma Rongchen Li Qiuyue Zhang Yugang Liu |
| author_facet | Wen Yang Ling Liu Jianliang Li Yingchao Liu Lin Rong Junjie Ma Rongchen Li Qiuyue Zhang Yugang Liu |
| author_sort | Wen Yang |
| collection | DOAJ |
| description | Abstract Background The accuracy and reliability of identified biomarkers in differentiating early non-small cell lung cancer (NSCLC) remain suboptimal, thereby impeding the timely detection of NSCLC.The objective of this research is to examine the expression level and diagnostic utility of miR-668-3p in individuals with NSCLC, along with its effectiveness and predictive capacity in the combined diagnosis of early-stage NSCLC using serum markers. Methods The research included 117 NSCLC patients and 101 pulmonary nodule patients (controls). Quantitative PCR was employed to assess the expression levels of miR-668-3p in NSCLC patients. The association between miR-668-3p and clinical characteristics and serum biomarker (AFP, CEA, NSE, and CYFRA21-1) levels in NSCLC patients was examined using chi-square tests and Pearson correlation analyses. The ROC curve analysis was conducted to determine the individual and combined diagnostic efficacy of miR-668-3p and serum biomarkers. Additionally, a logistic regression model was utilized to identify risk factors for lung cancer in patients with pulmonary tuberculosis. Results The expression level of miR-668-3p was down-regulated in early-stage NSCLC patients compared with the control group, and showed a significant association with serum biomarkers related with disease progression, tumor staging, and lymph node metastasis. The combined detection of miR-668-3p and serum markers demonstrated robust diagnostic efficacy for early NSCLC and effective predictive capabilities for lung cancer occurrence in individuals with pulmonary nodules. Conclusions The miR-668-3p has the potential to be a promising biomarker for NSCLC and enhance the accuracy of early NSCLC clinical detection. |
| format | Article |
| id | doaj-art-3d30bcd05b004390af4bb0fff8380491 |
| institution | Kabale University |
| issn | 1749-8090 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Cardiothoracic Surgery |
| spelling | doaj-art-3d30bcd05b004390af4bb0fff83804912025-01-12T12:38:51ZengBMCJournal of Cardiothoracic Surgery1749-80902025-01-012011910.1186/s13019-024-03220-wAbnormal expression of miR-668-3p in non-small cell lung cancer patients and its correlation with serum-related tumor markersWen Yang0Ling Liu1Jianliang Li2Yingchao Liu3Lin Rong4Junjie Ma5Rongchen Li6Qiuyue Zhang7Yugang Liu8Department of Pathology, Guangdong Provincial Key Laboratory of Major Obstetric Disease, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical UniversityDepartment of Pulmonary and Critical Care Medicine, Chongqing Southwest Aluminum HospitalDepartment of Thoracic Surgery, The Second People’s Hospital of LiaochengDepartment of Clinical Laboratory, The Second People’s Hospital of LiaochengDepartment of Thoracic Surgery, The Second People’s Hospital of LiaochengDepartment of Thoracic Surgery, The Second People’s Hospital of LiaochengDepartment of Clinical Laboratory, The Second People’s Hospital of LiaochengDepartment of Clinical Laboratory, The Second People’s Hospital of LiaochengDepartment of Oncology, The 969th Hospital of the PLA joint Logistics Support ForceAbstract Background The accuracy and reliability of identified biomarkers in differentiating early non-small cell lung cancer (NSCLC) remain suboptimal, thereby impeding the timely detection of NSCLC.The objective of this research is to examine the expression level and diagnostic utility of miR-668-3p in individuals with NSCLC, along with its effectiveness and predictive capacity in the combined diagnosis of early-stage NSCLC using serum markers. Methods The research included 117 NSCLC patients and 101 pulmonary nodule patients (controls). Quantitative PCR was employed to assess the expression levels of miR-668-3p in NSCLC patients. The association between miR-668-3p and clinical characteristics and serum biomarker (AFP, CEA, NSE, and CYFRA21-1) levels in NSCLC patients was examined using chi-square tests and Pearson correlation analyses. The ROC curve analysis was conducted to determine the individual and combined diagnostic efficacy of miR-668-3p and serum biomarkers. Additionally, a logistic regression model was utilized to identify risk factors for lung cancer in patients with pulmonary tuberculosis. Results The expression level of miR-668-3p was down-regulated in early-stage NSCLC patients compared with the control group, and showed a significant association with serum biomarkers related with disease progression, tumor staging, and lymph node metastasis. The combined detection of miR-668-3p and serum markers demonstrated robust diagnostic efficacy for early NSCLC and effective predictive capabilities for lung cancer occurrence in individuals with pulmonary nodules. Conclusions The miR-668-3p has the potential to be a promising biomarker for NSCLC and enhance the accuracy of early NSCLC clinical detection.https://doi.org/10.1186/s13019-024-03220-wNon-small cell lung cancermiRNATumor markerDiagnosticCombined detection |
| spellingShingle | Wen Yang Ling Liu Jianliang Li Yingchao Liu Lin Rong Junjie Ma Rongchen Li Qiuyue Zhang Yugang Liu Abnormal expression of miR-668-3p in non-small cell lung cancer patients and its correlation with serum-related tumor markers Journal of Cardiothoracic Surgery Non-small cell lung cancer miRNA Tumor marker Diagnostic Combined detection |
| title | Abnormal expression of miR-668-3p in non-small cell lung cancer patients and its correlation with serum-related tumor markers |
| title_full | Abnormal expression of miR-668-3p in non-small cell lung cancer patients and its correlation with serum-related tumor markers |
| title_fullStr | Abnormal expression of miR-668-3p in non-small cell lung cancer patients and its correlation with serum-related tumor markers |
| title_full_unstemmed | Abnormal expression of miR-668-3p in non-small cell lung cancer patients and its correlation with serum-related tumor markers |
| title_short | Abnormal expression of miR-668-3p in non-small cell lung cancer patients and its correlation with serum-related tumor markers |
| title_sort | abnormal expression of mir 668 3p in non small cell lung cancer patients and its correlation with serum related tumor markers |
| topic | Non-small cell lung cancer miRNA Tumor marker Diagnostic Combined detection |
| url | https://doi.org/10.1186/s13019-024-03220-w |
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