Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signaling
Background Apium graveolens L. (celery) is a dietary vegetable with anti-inflammatory properties. It has the potential to treat acute lung injury (ALI) caused by COVID-19 or other diseases.Objective To investigate the effects of Apium graveolens water extract (AGWE) on ALI in human lung A-549 cells...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2024.2433994 |
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| author | Lan-Chi Hsieh Shu-Ling Hsieh Tsu-Ni Ping Yi-Chun Huang Ssu-Jung Lin Hsing-Yu Chi Chih-Chung Wu |
| author_facet | Lan-Chi Hsieh Shu-Ling Hsieh Tsu-Ni Ping Yi-Chun Huang Ssu-Jung Lin Hsing-Yu Chi Chih-Chung Wu |
| author_sort | Lan-Chi Hsieh |
| collection | DOAJ |
| description | Background Apium graveolens L. (celery) is a dietary vegetable with anti-inflammatory properties. It has the potential to treat acute lung injury (ALI) caused by COVID-19 or other diseases.Objective To investigate the effects of Apium graveolens water extract (AGWE) on ALI in human lung A-549 cells induced by lipopolysaccharide (LPS).Materials and methods A-549 cells were treated with AGWE for 24 h and then stimulated with 10 μg/mL LPS for another 24 h. The effects of AGWE on cell viability, the inflammatory response, oxidative stress, and apoptosis and their regulatory factors, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and NLR family pyrin domain containing 3 (NLRP3) inflammasome signaling activation were analyzed.Results Treatment with 5–50 μg/mL AGWE reversed the decrease in cell viability caused by LPS (p < 0.05). AGWE can reduce interleukin (IL)-1β, IL-6, IL-18, and TNF-α levels; their EC50 values are 61.4, 65.7, 37.8, and 79.7 μg/mL, respectively. AGWE can reduce reactive oxygen species and thiobarbituric acid reactive substances in A-549 cells induced by LPS. AGWE also reduced the levels of apoptosis (EC50 of 74.8 μg/mL) and its regulators (Bid; Caspase-9, −8, and −3; Bax) and increased the levels of the mitochondrial membrane potential in A-549 cells induced by LPS. AGWE can also decrease the protein levels of NLRP3 and Caspase-1 and the activation of NF-κB signaling in A-549 cells induced by LPS.Conclusions These results show that 10 and 50 μg/mL AGWE can reduce the acute inflammation induced by LPS by reducing NF-κB and NLRP3 inflammasome signaling and mitochondria-dependent apoptosis pathways. |
| format | Article |
| id | doaj-art-3d11a05c3cc245e3859d634de9b0f899 |
| institution | Kabale University |
| issn | 1388-0209 1744-5116 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj-art-3d11a05c3cc245e3859d634de9b0f8992024-12-13T13:24:04ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162025-12-0163111310.1080/13880209.2024.2433994Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signalingLan-Chi Hsieh0Shu-Ling Hsieh1Tsu-Ni Ping2Yi-Chun Huang3Ssu-Jung Lin4Hsing-Yu Chi5Chih-Chung Wu6Department of Dietetics, Kaohsiung Municipal United Hospital, Kaohsiung, Taiwan, R.O.CDepartment of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan, R.O.CDepartment of Food and Nutrition, Providence University, Taichung, Taiwan, R.O.CDepartment of Food and Nutrition, Providence University, Taichung, Taiwan, R.O.CDepartment of Food and Nutrition, Providence University, Taichung, Taiwan, R.O.CDepartment of Food and Nutrition, Providence University, Taichung, Taiwan, R.O.CDepartment of Food and Nutrition, Providence University, Taichung, Taiwan, R.O.CBackground Apium graveolens L. (celery) is a dietary vegetable with anti-inflammatory properties. It has the potential to treat acute lung injury (ALI) caused by COVID-19 or other diseases.Objective To investigate the effects of Apium graveolens water extract (AGWE) on ALI in human lung A-549 cells induced by lipopolysaccharide (LPS).Materials and methods A-549 cells were treated with AGWE for 24 h and then stimulated with 10 μg/mL LPS for another 24 h. The effects of AGWE on cell viability, the inflammatory response, oxidative stress, and apoptosis and their regulatory factors, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and NLR family pyrin domain containing 3 (NLRP3) inflammasome signaling activation were analyzed.Results Treatment with 5–50 μg/mL AGWE reversed the decrease in cell viability caused by LPS (p < 0.05). AGWE can reduce interleukin (IL)-1β, IL-6, IL-18, and TNF-α levels; their EC50 values are 61.4, 65.7, 37.8, and 79.7 μg/mL, respectively. AGWE can reduce reactive oxygen species and thiobarbituric acid reactive substances in A-549 cells induced by LPS. AGWE also reduced the levels of apoptosis (EC50 of 74.8 μg/mL) and its regulators (Bid; Caspase-9, −8, and −3; Bax) and increased the levels of the mitochondrial membrane potential in A-549 cells induced by LPS. AGWE can also decrease the protein levels of NLRP3 and Caspase-1 and the activation of NF-κB signaling in A-549 cells induced by LPS.Conclusions These results show that 10 and 50 μg/mL AGWE can reduce the acute inflammation induced by LPS by reducing NF-κB and NLRP3 inflammasome signaling and mitochondria-dependent apoptosis pathways.https://www.tandfonline.com/doi/10.1080/13880209.2024.2433994Apium graveolens L. (celery;)acute lung injuryapoptosisNF-κBNLRP3 inflammasome |
| spellingShingle | Lan-Chi Hsieh Shu-Ling Hsieh Tsu-Ni Ping Yi-Chun Huang Ssu-Jung Lin Hsing-Yu Chi Chih-Chung Wu Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signaling Pharmaceutical Biology Apium graveolens L. (celery;) acute lung injury apoptosis NF-κB NLRP3 inflammasome |
| title | Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signaling |
| title_full | Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signaling |
| title_fullStr | Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signaling |
| title_full_unstemmed | Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signaling |
| title_short | Apium graveolens L. alleviates acute lung injury in human A-549 cells by reducing NF-κB and NLRP3 inflammasome signaling |
| title_sort | apium graveolens l alleviates acute lung injury in human a 549 cells by reducing nf κb and nlrp3 inflammasome signaling |
| topic | Apium graveolens L. (celery;) acute lung injury apoptosis NF-κB NLRP3 inflammasome |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2024.2433994 |
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