An additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption application
Additive manufacturing of hydrogels is a rapidly evolving field due to the unique properties of hydrogels and their potential applications in various sectors. However, the low production rate and coarse resolution of current additive manufacturing methods limit their use. This article proposes a Ste...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127525000012 |
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| author | Feng Cao Hossein Najaf Zadeh Klaudia Świacka Jakub Maculewicz Dan Bowles Tim Huber Don Clucas |
| author_facet | Feng Cao Hossein Najaf Zadeh Klaudia Świacka Jakub Maculewicz Dan Bowles Tim Huber Don Clucas |
| author_sort | Feng Cao |
| collection | DOAJ |
| description | Additive manufacturing of hydrogels is a rapidly evolving field due to the unique properties of hydrogels and their potential applications in various sectors. However, the low production rate and coarse resolution of current additive manufacturing methods limit their use. This article proposes a Stencil Additive Manufacturing (SAM) method to produce agarose hydrogel structures with horizontal and vertical resolutions of 500 and 80 μm using a novel SAM printer. Compared to peer methods, the shape fidelity of printed structures was improved and errors resulting from the Barus effect were minimized to 1.7 % and 7.1 %, depending on stencil patterns. Mechanical and thermal properties of agarose hydrogels were investigated by considering chemical crosslinking and agarose concentration, and the gelation and melting temperatures were determined. The analysis of hydrogel microstructures illustrated the change in porosity by regulating agarose concentration and the gelation rate. Static bovine serum albumin binding tests were performed using printed structures with varying concentrations and resolutions to explore the protein adsorption capacity. The results indicated that structure resolutions affect the adsorption capacity dramatically, which was increased from 100.44 to 144.13 mg/ml as resolutions were improved from 500 to 350 µm. Therefore, SAM-printing agarose hydrogels with periodic structures demonstrates potential in applications. |
| format | Article |
| id | doaj-art-3cda8fd15a55483f99c35f645b865ffc |
| institution | Kabale University |
| issn | 0264-1275 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj-art-3cda8fd15a55483f99c35f645b865ffc2025-01-13T04:18:21ZengElsevierMaterials & Design0264-12752025-02-01250113581An additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption applicationFeng Cao0Hossein Najaf Zadeh1Klaudia Świacka2Jakub Maculewicz3Dan Bowles4Tim Huber5Don Clucas6Biomolecular Interaction Centre, University of Canterbury, Christchurch 8041, New ZealandBiomolecular Interaction Centre, University of Canterbury, Christchurch 8041, New Zealand; School of Product Design, University of Canterbury, Private Bag 4800, Christchurch 8041, New Zealand; Corresponding author at: Biomolecular Interaction Centre, University of Canterbury, Christchurch 8041, New Zealand.Biomolecular Interaction Centre, University of Canterbury, Christchurch 8041, New ZealandBiomolecular Interaction Centre, University of Canterbury, Christchurch 8041, New ZealandSchool of Product Design, University of Canterbury, Private Bag 4800, Christchurch 8041, New ZealandLuxembourg Institute of Science and Technology, 5 Av. des Hauts-Fourneaux, 4362 Luxembourg, New ZealandDepartment of Mechanical Engineering, University of Canterbury, Christchurch 8041, New ZealandAdditive manufacturing of hydrogels is a rapidly evolving field due to the unique properties of hydrogels and their potential applications in various sectors. However, the low production rate and coarse resolution of current additive manufacturing methods limit their use. This article proposes a Stencil Additive Manufacturing (SAM) method to produce agarose hydrogel structures with horizontal and vertical resolutions of 500 and 80 μm using a novel SAM printer. Compared to peer methods, the shape fidelity of printed structures was improved and errors resulting from the Barus effect were minimized to 1.7 % and 7.1 %, depending on stencil patterns. Mechanical and thermal properties of agarose hydrogels were investigated by considering chemical crosslinking and agarose concentration, and the gelation and melting temperatures were determined. The analysis of hydrogel microstructures illustrated the change in porosity by regulating agarose concentration and the gelation rate. Static bovine serum albumin binding tests were performed using printed structures with varying concentrations and resolutions to explore the protein adsorption capacity. The results indicated that structure resolutions affect the adsorption capacity dramatically, which was increased from 100.44 to 144.13 mg/ml as resolutions were improved from 500 to 350 µm. Therefore, SAM-printing agarose hydrogels with periodic structures demonstrates potential in applications.http://www.sciencedirect.com/science/article/pii/S0264127525000012Stencil additive manufacturingAgarose hydrogelStatic protein bindingProtein adsorption |
| spellingShingle | Feng Cao Hossein Najaf Zadeh Klaudia Świacka Jakub Maculewicz Dan Bowles Tim Huber Don Clucas An additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption application Materials & Design Stencil additive manufacturing Agarose hydrogel Static protein binding Protein adsorption |
| title | An additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption application |
| title_full | An additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption application |
| title_fullStr | An additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption application |
| title_full_unstemmed | An additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption application |
| title_short | An additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption application |
| title_sort | additive manufacturing approach for fabrication of agarose hydrogel structures for protein sorption application |
| topic | Stencil additive manufacturing Agarose hydrogel Static protein binding Protein adsorption |
| url | http://www.sciencedirect.com/science/article/pii/S0264127525000012 |
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