Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division
Abstract A key challenge for bottom-up synthetic biology is engineering a minimal module for self-division of synthetic cells. Actin-based cytokinetic rings are considered a promising structure to produce the forces required for the controlled excision of cell-like compartments such as giant unilame...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-54807-9 |
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| _version_ | 1846147660098043904 |
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| author | María Reverte-López Nishu Kanwa Yusuf Qutbuddin Viktoriia Belousova Marion Jasnin Petra Schwille |
| author_facet | María Reverte-López Nishu Kanwa Yusuf Qutbuddin Viktoriia Belousova Marion Jasnin Petra Schwille |
| author_sort | María Reverte-López |
| collection | DOAJ |
| description | Abstract A key challenge for bottom-up synthetic biology is engineering a minimal module for self-division of synthetic cells. Actin-based cytokinetic rings are considered a promising structure to produce the forces required for the controlled excision of cell-like compartments such as giant unilamellar vesicles (GUVs). Despite prior demonstrations of actin ring targeting to GUV membranes and myosin-induced constriction, large-scale vesicle deformation has been precluded due to the lacking spatial control of these contractile structures. Here we show the combined reconstitution of actomyosin rings and the bacterial MinDE protein system within GUVs. Incorporating this spatial positioning tool, able to induce active transport of membrane-attached diffusible molecules, yields self-organized equatorial assembly of actomyosin rings in vesicles. Remarkably, the synergistic effect of Min oscillations and the contractility of actomyosin bundles induces mid-vesicle deformations and vesicle blebbing. Our system showcases how functional machineries from various organisms may be combined in vitro, leading to the emergence of functionalities towards a synthetic division system. |
| format | Article |
| id | doaj-art-3ca0f63a8d7c4e0bb4b1a559b2d8f80c |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-3ca0f63a8d7c4e0bb4b1a559b2d8f80c2024-12-01T12:33:24ZengNature PortfolioNature Communications2041-17232024-11-0115111310.1038/s41467-024-54807-9Self-organized spatial targeting of contractile actomyosin rings for synthetic cell divisionMaría Reverte-López0Nishu Kanwa1Yusuf Qutbuddin2Viktoriia Belousova3Marion Jasnin4Petra Schwille5Department of Cellular and Molecular Biophysics, Max Planck Institute of BiochemistryDepartment of Cellular and Molecular Biophysics, Max Planck Institute of BiochemistryDepartment of Cellular and Molecular Biophysics, Max Planck Institute of BiochemistryDepartment of Cellular and Molecular Biophysics, Max Planck Institute of BiochemistryHelmholtz Pioneer Campus, Helmholtz Munich, Neuherberg, Germany; Department of Chemistry, Technical University of MunichDepartment of Cellular and Molecular Biophysics, Max Planck Institute of BiochemistryAbstract A key challenge for bottom-up synthetic biology is engineering a minimal module for self-division of synthetic cells. Actin-based cytokinetic rings are considered a promising structure to produce the forces required for the controlled excision of cell-like compartments such as giant unilamellar vesicles (GUVs). Despite prior demonstrations of actin ring targeting to GUV membranes and myosin-induced constriction, large-scale vesicle deformation has been precluded due to the lacking spatial control of these contractile structures. Here we show the combined reconstitution of actomyosin rings and the bacterial MinDE protein system within GUVs. Incorporating this spatial positioning tool, able to induce active transport of membrane-attached diffusible molecules, yields self-organized equatorial assembly of actomyosin rings in vesicles. Remarkably, the synergistic effect of Min oscillations and the contractility of actomyosin bundles induces mid-vesicle deformations and vesicle blebbing. Our system showcases how functional machineries from various organisms may be combined in vitro, leading to the emergence of functionalities towards a synthetic division system.https://doi.org/10.1038/s41467-024-54807-9 |
| spellingShingle | María Reverte-López Nishu Kanwa Yusuf Qutbuddin Viktoriia Belousova Marion Jasnin Petra Schwille Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division Nature Communications |
| title | Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division |
| title_full | Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division |
| title_fullStr | Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division |
| title_full_unstemmed | Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division |
| title_short | Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division |
| title_sort | self organized spatial targeting of contractile actomyosin rings for synthetic cell division |
| url | https://doi.org/10.1038/s41467-024-54807-9 |
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