Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits
Thiram (THI) and asomate (ASO) are frequently detected agrochemicals in food samples due to widespread use as fungicides. However, their detection suffers from matrix interference, or the necessity of derivatization before measurement. Integrating HPLC with a post-column carbon dots (CDs)–enhanced c...
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Elsevier
2024-12-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524007673 |
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| author | Lijun Wei Mengdie Cai Bixiao Zhou Ping Yuan Guowen Zhang Xianglei Cheng |
| author_facet | Lijun Wei Mengdie Cai Bixiao Zhou Ping Yuan Guowen Zhang Xianglei Cheng |
| author_sort | Lijun Wei |
| collection | DOAJ |
| description | Thiram (THI) and asomate (ASO) are frequently detected agrochemicals in food samples due to widespread use as fungicides. However, their detection suffers from matrix interference, or the necessity of derivatization before measurement. Integrating HPLC with a post-column carbon dots (CDs)–enhanced chemiluminescence (CL) system makes it possible to simultaneously separate and selectively detect ASO and THI in fruit samples free of derivatization and deep purification. The studies on mechanisms demonstrated that CD intermediates of anionic radical were responsible for the remarkable CL enhancement of ASO or THI. The CL intensity is directly proportional to the fungicide concentration, with the ranges of linearity for ASO and THI being 5–250 μg·L−1 (R2 = 0.9993) and 4–200 μg·L−1 (R2 = 0.9986), respectively, and the corresponding limits of detection (S/N = 3) being 1.61 and 1.33 μg·L−1, respectively. The developed method enables the exploration of the novelly sulphur-targeted CL application based on carbon nanomaterials. |
| format | Article |
| id | doaj-art-3c8cf9b24ceb460c9044036b12cd8e6f |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-3c8cf9b24ceb460c9044036b12cd8e6f2024-12-13T11:01:38ZengElsevierFood Chemistry: X2590-15752024-12-0124101879Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruitsLijun Wei0Mengdie Cai1Bixiao Zhou2Ping Yuan3Guowen Zhang4Xianglei Cheng5State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China; Jiangxi Province Key Laboratory of Disease Prevention and Public Health, School of Public Health, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaJiangxi Province Key Laboratory of Disease Prevention and Public Health, School of Public Health, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaJiangxi Province Key Laboratory of Disease Prevention and Public Health, School of Public Health, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaJiangxi Province Key Laboratory of Disease Prevention and Public Health, School of Public Health, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China; Corresponding author at: State Key Laboratory of Food Science and Resources, Nanchang University, 235 Nanjing East Road, Nanchang city, Jiangxi province 330047, PR China.Jiangxi Province Key Laboratory of Disease Prevention and Public Health, School of Public Health, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR China; Corresponding author at: School of Public Health, Nanchang University, 461 Bayi Road, Nanchang 330006, PR China.Thiram (THI) and asomate (ASO) are frequently detected agrochemicals in food samples due to widespread use as fungicides. However, their detection suffers from matrix interference, or the necessity of derivatization before measurement. Integrating HPLC with a post-column carbon dots (CDs)–enhanced chemiluminescence (CL) system makes it possible to simultaneously separate and selectively detect ASO and THI in fruit samples free of derivatization and deep purification. The studies on mechanisms demonstrated that CD intermediates of anionic radical were responsible for the remarkable CL enhancement of ASO or THI. The CL intensity is directly proportional to the fungicide concentration, with the ranges of linearity for ASO and THI being 5–250 μg·L−1 (R2 = 0.9993) and 4–200 μg·L−1 (R2 = 0.9986), respectively, and the corresponding limits of detection (S/N = 3) being 1.61 and 1.33 μg·L−1, respectively. The developed method enables the exploration of the novelly sulphur-targeted CL application based on carbon nanomaterials.http://www.sciencedirect.com/science/article/pii/S2590157524007673ThiramAsomateN-doped carbon dotsSulphur-selective chemiluminescence |
| spellingShingle | Lijun Wei Mengdie Cai Bixiao Zhou Ping Yuan Guowen Zhang Xianglei Cheng Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits Food Chemistry: X Thiram Asomate N-doped carbon dots Sulphur-selective chemiluminescence |
| title | Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits |
| title_full | Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits |
| title_fullStr | Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits |
| title_full_unstemmed | Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits |
| title_short | Novel sulphur-selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits |
| title_sort | novel sulphur selective method for simultaneous determination of thiram and asomate based on carbon dots and its application in fruits |
| topic | Thiram Asomate N-doped carbon dots Sulphur-selective chemiluminescence |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524007673 |
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