Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples

Abstract Objective In the early stages of food production, detection of Aspergillus species contamination is crucial to prevent potential harm to human health. The primary focus of this study was to evaluate the performance of Multiplex Real-Time PCR (MRT-PCR) and melting curve analysis (MCA) in ide...

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Bibliographic Details
Main Authors: Mahtab Garousin, Reza Habibipour
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Research Notes
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Online Access:https://doi.org/10.1186/s13104-025-07341-w
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