Expression profiles of miR-101-3p and miR-431-5p as potential diagnostic biomarkers for rheumatoid arthritis
Abstract Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent inflammation of the synovial joints, leading to cartilage and bone destruction. This study aimed to evaluate the diagnostic utility of specific microRNAs (miRNAs) as potential biomarkers for RA. The study...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-82339-1 |
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| author | Mohamed M. Sadaty Salma M. Mekhemer Shaimaa Abdel-Ghany Amira R. El-Ansary Rana Mohamed Nashaat N. Kamal Hussein Sabit |
| author_facet | Mohamed M. Sadaty Salma M. Mekhemer Shaimaa Abdel-Ghany Amira R. El-Ansary Rana Mohamed Nashaat N. Kamal Hussein Sabit |
| author_sort | Mohamed M. Sadaty |
| collection | DOAJ |
| description | Abstract Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent inflammation of the synovial joints, leading to cartilage and bone destruction. This study aimed to evaluate the diagnostic utility of specific microRNAs (miRNAs) as potential biomarkers for RA. The study was conducted on 60 patients with RA disease along with 20 control participants. Comprehensive analysis of patient data, encompassing serological, hematological, and biochemical markers, revealed significantly elevated levels of miR-99b-5p, miR-101-3p, and miR-431-5p in RA patients compared to healthy controls. Among these, miR-101-3p demonstrated the highest diagnostic accuracy, with an area under the curve (AUC) of 0.873. These findings contribute to a deeper understanding of RA pathogenesis and suggest that miR-101-3p may serve as a valuable biomarker for early disease detection and potentially improved patient management. Further research is warranted to elucidate the precise mechanisms underlying miRNA involvement in RA and to explore their potential as therapeutic targets. |
| format | Article |
| id | doaj-art-3be3e13efb9144f0a25450c74741a1f7 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-3be3e13efb9144f0a25450c74741a1f72025-01-05T12:19:19ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-024-82339-1Expression profiles of miR-101-3p and miR-431-5p as potential diagnostic biomarkers for rheumatoid arthritisMohamed M. Sadaty0Salma M. Mekhemer1Shaimaa Abdel-Ghany2Amira R. El-Ansary3Rana Mohamed4Nashaat N. Kamal5Hussein Sabit6Department of Technology of Medical Laboratory, Faculty of Applied Health Science Technology, Misr University for Science and TechnologyDepartment of Technology of Medical Laboratory, Faculty of Applied Health Science Technology, Misr University for Science and TechnologyDepartment of Environmental Biotechnology, College of Biotechnology, Misr University for Science and TechnologyDepartment of Internal Medicine, Faculty of Medicine, Misr University for Science and TechnologyDepartment of Technology of Medical Laboratory, Faculty of Applied Health Science Technology, Misr University for Science and TechnologyDepartment of Medical Biotechnology, College of Biotechnology, Misr University for Science and TechnologyDepartment of Medical Biotechnology, College of Biotechnology, Misr University for Science and TechnologyAbstract Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent inflammation of the synovial joints, leading to cartilage and bone destruction. This study aimed to evaluate the diagnostic utility of specific microRNAs (miRNAs) as potential biomarkers for RA. The study was conducted on 60 patients with RA disease along with 20 control participants. Comprehensive analysis of patient data, encompassing serological, hematological, and biochemical markers, revealed significantly elevated levels of miR-99b-5p, miR-101-3p, and miR-431-5p in RA patients compared to healthy controls. Among these, miR-101-3p demonstrated the highest diagnostic accuracy, with an area under the curve (AUC) of 0.873. These findings contribute to a deeper understanding of RA pathogenesis and suggest that miR-101-3p may serve as a valuable biomarker for early disease detection and potentially improved patient management. Further research is warranted to elucidate the precise mechanisms underlying miRNA involvement in RA and to explore their potential as therapeutic targets.https://doi.org/10.1038/s41598-024-82339-1Rheumatoid arthritis (RA)Diagnostic biomarkersmiR-99b-5pmiR-101-3pmiR-431-5p |
| spellingShingle | Mohamed M. Sadaty Salma M. Mekhemer Shaimaa Abdel-Ghany Amira R. El-Ansary Rana Mohamed Nashaat N. Kamal Hussein Sabit Expression profiles of miR-101-3p and miR-431-5p as potential diagnostic biomarkers for rheumatoid arthritis Scientific Reports Rheumatoid arthritis (RA) Diagnostic biomarkers miR-99b-5p miR-101-3p miR-431-5p |
| title | Expression profiles of miR-101-3p and miR-431-5p as potential diagnostic biomarkers for rheumatoid arthritis |
| title_full | Expression profiles of miR-101-3p and miR-431-5p as potential diagnostic biomarkers for rheumatoid arthritis |
| title_fullStr | Expression profiles of miR-101-3p and miR-431-5p as potential diagnostic biomarkers for rheumatoid arthritis |
| title_full_unstemmed | Expression profiles of miR-101-3p and miR-431-5p as potential diagnostic biomarkers for rheumatoid arthritis |
| title_short | Expression profiles of miR-101-3p and miR-431-5p as potential diagnostic biomarkers for rheumatoid arthritis |
| title_sort | expression profiles of mir 101 3p and mir 431 5p as potential diagnostic biomarkers for rheumatoid arthritis |
| topic | Rheumatoid arthritis (RA) Diagnostic biomarkers miR-99b-5p miR-101-3p miR-431-5p |
| url | https://doi.org/10.1038/s41598-024-82339-1 |
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