Co‐Occurrence Patterns Do Not Predict Mutualistic Interactions Between Plant and Butterfly Species

ABSTRACT Biotic interactions are crucial for determining the structure and dynamics of communities; however, direct measurement of these interactions can be challenging in terms of time and resources, especially when numerous species are involved. Inferring species interactions from species co‐occur...

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Main Authors: Esteban Menares, Hugo Saíz, Noëlle Schenk, Enrique G. de laRiva, Jochen Krauss, Klaus Birkhofer
Format: Article
Language:English
Published: Wiley 2024-11-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.70498
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author Esteban Menares
Hugo Saíz
Noëlle Schenk
Enrique G. de laRiva
Jochen Krauss
Klaus Birkhofer
author_facet Esteban Menares
Hugo Saíz
Noëlle Schenk
Enrique G. de laRiva
Jochen Krauss
Klaus Birkhofer
author_sort Esteban Menares
collection DOAJ
description ABSTRACT Biotic interactions are crucial for determining the structure and dynamics of communities; however, direct measurement of these interactions can be challenging in terms of time and resources, especially when numerous species are involved. Inferring species interactions from species co‐occurrence patterns is increasingly being used; however, recent studies have highlighted some limitations. To our knowledge, no attempt has been made to test the accuracy of the existing methods for detecting mutualistic interactions in terrestrial ecosystems. In this study, we compiled two literature‐based, long‐term datasets of interactions between butterflies and herbaceous plant species in two regions of Germany and compared them with observational abundance and presence/absence data collected within a year in the same regions. We tested how well the species associations generated by three different co‐occurrence analysis methods matched those of empirically measured mutualistic associations using sensitivity and specificity analyses and compared the strength of associations. We also checked whether flower abundance data (instead of plant abundance data) increased the accuracy of the co‐occurrence models and validated our results using empirical flower visitation data. The results revealed that, although all methods exhibited low sensitivity, our implementation of the Relative Interaction Intensity index with pairwise null models performed the best, followed by the probabilistic method and Spearman's rank correlation method. However, empirical data showed a significant number of interactions that were not detected using co‐occurrence methods. Incorporating flower abundance data did not improve sensitivity but enhanced specificity in one region. Further analysis demonstrated incongruence between the predicted co‐occurrence associations and actual interaction strengths, with many pairs exhibiting high interaction strength but low co‐occurrence or vice versa. These findings underscore the complexity of ecological dynamics and highlight the limitations of current co‐occurrence methods for accurately capturing species interactions.
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spelling doaj-art-3bbaaf2ea60b42c2a6166265cbd3e9502024-12-20T04:20:57ZengWileyEcology and Evolution2045-77582024-11-011411n/an/a10.1002/ece3.70498Co‐Occurrence Patterns Do Not Predict Mutualistic Interactions Between Plant and Butterfly SpeciesEsteban Menares0Hugo Saíz1Noëlle Schenk2Enrique G. de laRiva3Jochen Krauss4Klaus Birkhofer5Department of Ecology Brandenburg University of Technology Cottbus‐Senftenberg Cottbus GermanyInstitute of Plant Sciences University of Bern Bern SwitzerlandInstitute of Plant Sciences University of Bern Bern SwitzerlandDepartment of Ecology Brandenburg University of Technology Cottbus‐Senftenberg Cottbus GermanyDepartment of Animal Ecology and Tropical Biology University of Würzburg Würzburg GermanyDepartment of Ecology Brandenburg University of Technology Cottbus‐Senftenberg Cottbus GermanyABSTRACT Biotic interactions are crucial for determining the structure and dynamics of communities; however, direct measurement of these interactions can be challenging in terms of time and resources, especially when numerous species are involved. Inferring species interactions from species co‐occurrence patterns is increasingly being used; however, recent studies have highlighted some limitations. To our knowledge, no attempt has been made to test the accuracy of the existing methods for detecting mutualistic interactions in terrestrial ecosystems. In this study, we compiled two literature‐based, long‐term datasets of interactions between butterflies and herbaceous plant species in two regions of Germany and compared them with observational abundance and presence/absence data collected within a year in the same regions. We tested how well the species associations generated by three different co‐occurrence analysis methods matched those of empirically measured mutualistic associations using sensitivity and specificity analyses and compared the strength of associations. We also checked whether flower abundance data (instead of plant abundance data) increased the accuracy of the co‐occurrence models and validated our results using empirical flower visitation data. The results revealed that, although all methods exhibited low sensitivity, our implementation of the Relative Interaction Intensity index with pairwise null models performed the best, followed by the probabilistic method and Spearman's rank correlation method. However, empirical data showed a significant number of interactions that were not detected using co‐occurrence methods. Incorporating flower abundance data did not improve sensitivity but enhanced specificity in one region. Further analysis demonstrated incongruence between the predicted co‐occurrence associations and actual interaction strengths, with many pairs exhibiting high interaction strength but low co‐occurrence or vice versa. These findings underscore the complexity of ecological dynamics and highlight the limitations of current co‐occurrence methods for accurately capturing species interactions.https://doi.org/10.1002/ece3.70498Biodiversity Exploratoriesco‐occurrence analysisflower visitationsmutualistic interactionspresence/absence dataspecies associations
spellingShingle Esteban Menares
Hugo Saíz
Noëlle Schenk
Enrique G. de laRiva
Jochen Krauss
Klaus Birkhofer
Co‐Occurrence Patterns Do Not Predict Mutualistic Interactions Between Plant and Butterfly Species
Ecology and Evolution
Biodiversity Exploratories
co‐occurrence analysis
flower visitations
mutualistic interactions
presence/absence data
species associations
title Co‐Occurrence Patterns Do Not Predict Mutualistic Interactions Between Plant and Butterfly Species
title_full Co‐Occurrence Patterns Do Not Predict Mutualistic Interactions Between Plant and Butterfly Species
title_fullStr Co‐Occurrence Patterns Do Not Predict Mutualistic Interactions Between Plant and Butterfly Species
title_full_unstemmed Co‐Occurrence Patterns Do Not Predict Mutualistic Interactions Between Plant and Butterfly Species
title_short Co‐Occurrence Patterns Do Not Predict Mutualistic Interactions Between Plant and Butterfly Species
title_sort co occurrence patterns do not predict mutualistic interactions between plant and butterfly species
topic Biodiversity Exploratories
co‐occurrence analysis
flower visitations
mutualistic interactions
presence/absence data
species associations
url https://doi.org/10.1002/ece3.70498
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