Silk‐Fibroin as Biocompatible and Bioresorbable Enzyme Immobilization Matrix for Screen‐Printed Amperometric Glucose Biosensors
Abstract Silk‐fibroin is utilized as a biocompatible and bioresorbable enzyme immobilization matrix and exemplarily demonstrated for a screen‐printed amperometric glucose biosensor. The silk‐fibroin is derived from the silkworm Bombyx mori. The enzyme immobilization matrix consisting of silk‐fibroin...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-08-01
|
| Series: | Advanced Sensor Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/adsr.202500048 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Abstract Silk‐fibroin is utilized as a biocompatible and bioresorbable enzyme immobilization matrix and exemplarily demonstrated for a screen‐printed amperometric glucose biosensor. The silk‐fibroin is derived from the silkworm Bombyx mori. The enzyme immobilization matrix consisting of silk‐fibroin, together with glucose oxidase from Aspergillus niger, is applied to a screen‐printed carbon‐based, biocompatible, and biodegradable working electrode on a flexible silk‐fibroin substrate. The biosensor is characterized electrochemically at physiological glucose concentrations in the range from 0.5 to 10 mm. The results are compared to a “conventional” glucose biosensor, also fabricated on a flexible silk‐fibroin substrate, however utilizing a laboratory standard enzyme immobilization matrix based on bovine serum albumin and glutaraldehyde. Furthermore, the influence of pH (pH 5.5 to pH 8.0) and temperature variations (21 to 70 °C) on these two different immobilization matrices are studied. |
|---|---|
| ISSN: | 2751-1219 |