Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors
Summary: ONE vector G protein optical (ONE-GO) biosensors are versatile tools to measure the activity of G protein-coupled receptors (GPCRs) in cells. The availability of ONE-GO biosensors for ten active Gα subunits representative of all four G protein families (Gs, Gi/o, Gq/11, and G12/13) permits...
Saved in:
Main Authors: | Remi Janicot, Mikel Garcia-Marcos |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2024-12-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724005483 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Protocol for detecting endogenous GPCR activity in primary cell cultures using ONE-GO biosensors
by: Remi Janicot, et al.
Published: (2024-12-01) -
Protocol for whole-cell patch-clamp assay for HBV-mediated sodium influx in THP-1 macrophages
by: Yingbao Tan, et al.
Published: (2024-12-01) -
Molecular details and phosphoregulation of the CENP-T-Mis12 complex interaction during mitosis in DT40 cells
by: Yusuke Takenoshita, et al.
Published: (2024-12-01) -
Protocol to measure cell avidity between cord blood-derived NK cells and leukemia cell line KG-1a
by: Yan Wang, et al.
Published: (2024-12-01) -
Rapid diffused optical imaging for accurate 3D estimation of subcutaneous tissue features
by: Shanshan Cai, et al.
Published: (2025-02-01)