Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors
Summary: ONE vector G protein optical (ONE-GO) biosensors are versatile tools to measure the activity of G protein-coupled receptors (GPCRs) in cells. The availability of ONE-GO biosensors for ten active Gα subunits representative of all four G protein families (Gs, Gi/o, Gq/11, and G12/13) permits...
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724005483 |
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| author | Remi Janicot Mikel Garcia-Marcos |
| author_facet | Remi Janicot Mikel Garcia-Marcos |
| author_sort | Remi Janicot |
| collection | DOAJ |
| description | Summary: ONE vector G protein optical (ONE-GO) biosensors are versatile tools to measure the activity of G protein-coupled receptors (GPCRs) in cells. The availability of ONE-GO biosensors for ten active Gα subunits representative of all four G protein families (Gs, Gi/o, Gq/11, and G12/13) permits the study of virtually any GPCR. Here, we present a protocol to implement ONE-GO biosensors in cell lines to investigate GPCR signaling kinetics and concentration-dependent responses. We describe steps for cell culture and transfection, response measurement, and data analysis.For complete details on the use and execution of this protocol, please refer to Janicot et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-3b8a4a232e604eea97c0671357e2b819 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-3b8a4a232e604eea97c0671357e2b8192024-11-20T05:08:01ZengElsevierSTAR Protocols2666-16672024-12-0154103383Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensorsRemi Janicot0Mikel Garcia-Marcos1Department of Biochemistry & Cell Biology, Chobanian & Avedisian School of Medicine, Boston University, Boston, MA 02118, USADepartment of Biochemistry & Cell Biology, Chobanian & Avedisian School of Medicine, Boston University, Boston, MA 02118, USA; Department of Biology, College of Arts & Sciences, Boston University, Boston, MA 02115, USA; Corresponding authorSummary: ONE vector G protein optical (ONE-GO) biosensors are versatile tools to measure the activity of G protein-coupled receptors (GPCRs) in cells. The availability of ONE-GO biosensors for ten active Gα subunits representative of all four G protein families (Gs, Gi/o, Gq/11, and G12/13) permits the study of virtually any GPCR. Here, we present a protocol to implement ONE-GO biosensors in cell lines to investigate GPCR signaling kinetics and concentration-dependent responses. We describe steps for cell culture and transfection, response measurement, and data analysis.For complete details on the use and execution of this protocol, please refer to Janicot et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724005483biophysicscell biologycell culturecell-based assaysmolecular biologysignal transduction |
| spellingShingle | Remi Janicot Mikel Garcia-Marcos Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors STAR Protocols biophysics cell biology cell culture cell-based assays molecular biology signal transduction |
| title | Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors |
| title_full | Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors |
| title_fullStr | Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors |
| title_full_unstemmed | Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors |
| title_short | Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors |
| title_sort | protocol to investigate g protein coupled receptor signaling kinetics and concentration dependent responses using one go biosensors |
| topic | biophysics cell biology cell culture cell-based assays molecular biology signal transduction |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724005483 |
| work_keys_str_mv | AT remijanicot protocoltoinvestigategproteincoupledreceptorsignalingkineticsandconcentrationdependentresponsesusingonegobiosensors AT mikelgarciamarcos protocoltoinvestigategproteincoupledreceptorsignalingkineticsandconcentrationdependentresponsesusingonegobiosensors |