A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease
Marburg virus disease (MVD) is a severe infectious disease caused by the Marburg virus (MARV), posing a significant threat to humans. MARV needs to be operated under strict biosafety Level 4 (BSL-4) laboratory conditions. Therefore, accessible and practical animal models are urgently needed to advan...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2024.2449083 |
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| author | Wanying Yang Wujie Zhou Bo Liang Xiaojun Hu Shen Wang Zhenshan Wang Tiecheng Wang Xianzhu Xia Na Feng Yongkun Zhao Feihu Yan |
| author_facet | Wanying Yang Wujie Zhou Bo Liang Xiaojun Hu Shen Wang Zhenshan Wang Tiecheng Wang Xianzhu Xia Na Feng Yongkun Zhao Feihu Yan |
| author_sort | Wanying Yang |
| collection | DOAJ |
| description | Marburg virus disease (MVD) is a severe infectious disease caused by the Marburg virus (MARV), posing a significant threat to humans. MARV needs to be operated under strict biosafety Level 4 (BSL-4) laboratory conditions. Therefore, accessible and practical animal models are urgently needed to advance prophylactic and therapeutic strategies for MARV. In this study, we constructed a recombinant vesicular stomatitis virus (VSV) expressing the Marburg virus glycoprotein (VSV-MARV/GP). Syrian hamsters infected with VSV-MARV/GP presented symptoms such as thrombocytopenia, lymphopenia, haemophilia, and multiorgan failure, developing a severe systemic disease akin to that observed in human MARV patients. Notably, the pathogenicity was found to be species-specific, age-related, sex-associated, and challenge route-dependent. Subsequently, the therapeutic efficacy of the MR191 monoclonal antibody was validated in this model. In summary, this alternative model is an effective tool for rapidly screening medical countermeasures against MARV GP in vivo under BSL-2 conditions. |
| format | Article |
| id | doaj-art-3b7a70580abc4f38b90b742d42a9fbcd |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-3b7a70580abc4f38b90b742d42a9fbcd2025-01-12T19:46:58ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512025-12-0114110.1080/22221751.2024.2449083A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus diseaseWanying Yang0Wujie Zhou1Bo Liang2Xiaojun Hu3Shen Wang4Zhenshan Wang5Tiecheng Wang6Xianzhu Xia7Na Feng8Yongkun Zhao9Feihu Yan10State Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaState Key Laboratory of Pathogenic Microorganisms, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, People’s Republic of ChinaMarburg virus disease (MVD) is a severe infectious disease caused by the Marburg virus (MARV), posing a significant threat to humans. MARV needs to be operated under strict biosafety Level 4 (BSL-4) laboratory conditions. Therefore, accessible and practical animal models are urgently needed to advance prophylactic and therapeutic strategies for MARV. In this study, we constructed a recombinant vesicular stomatitis virus (VSV) expressing the Marburg virus glycoprotein (VSV-MARV/GP). Syrian hamsters infected with VSV-MARV/GP presented symptoms such as thrombocytopenia, lymphopenia, haemophilia, and multiorgan failure, developing a severe systemic disease akin to that observed in human MARV patients. Notably, the pathogenicity was found to be species-specific, age-related, sex-associated, and challenge route-dependent. Subsequently, the therapeutic efficacy of the MR191 monoclonal antibody was validated in this model. In summary, this alternative model is an effective tool for rapidly screening medical countermeasures against MARV GP in vivo under BSL-2 conditions.https://www.tandfonline.com/doi/10.1080/22221751.2024.2449083Marburg virusrecombinant vesicular stomatitis virusSyrian hamstersurrogate modelrecurrence of classic symptomsvaccine evaluation and drug screening |
| spellingShingle | Wanying Yang Wujie Zhou Bo Liang Xiaojun Hu Shen Wang Zhenshan Wang Tiecheng Wang Xianzhu Xia Na Feng Yongkun Zhao Feihu Yan A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease Emerging Microbes and Infections Marburg virus recombinant vesicular stomatitis virus Syrian hamster surrogate model recurrence of classic symptoms vaccine evaluation and drug screening |
| title | A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease |
| title_full | A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease |
| title_fullStr | A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease |
| title_full_unstemmed | A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease |
| title_short | A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease |
| title_sort | surrogate bsl2 compliant infection model recapitulating key aspects of human marburg virus disease |
| topic | Marburg virus recombinant vesicular stomatitis virus Syrian hamster surrogate model recurrence of classic symptoms vaccine evaluation and drug screening |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2024.2449083 |
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