Antimicrobial susceptibility of clinically important carbapenem-resistant Enterobacterales towards standard and new antibiotics
AIM: The aim of this work is to study carbapenem-resistant Enterobacterales (CRE) incidence, carbapenemase types and antimicrobial susceptibility of the strains to standard and new antibiotics. BACKGROUND: CRE, carbapenemase–producers, are of critical importance (WHO, CDC), as determinants of diffic...
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524002108 |
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| author | Deniz Hamidov Emma Keuleyan Theodor Todorov Radoslava Vazharova Georgi Todorov Atanas Syarov |
| author_facet | Deniz Hamidov Emma Keuleyan Theodor Todorov Radoslava Vazharova Georgi Todorov Atanas Syarov |
| author_sort | Deniz Hamidov |
| collection | DOAJ |
| description | AIM: The aim of this work is to study carbapenem-resistant Enterobacterales (CRE) incidence, carbapenemase types and antimicrobial susceptibility of the strains to standard and new antibiotics. BACKGROUND: CRE, carbapenemase–producers, are of critical importance (WHO, CDC), as determinants of difficult to treat infections. Their spread should be controlled and new effective antibiotics are urgently needed. METHODS: During 2023, CRE from patients of the University hospital “Lozenetz” – Sofia were isolated from different clinical specimens and identified by MALDI-tof, Bruker, DE. Susceptibility testing (EUCAST) included Disc diffusion method, MIC determination by Phoenix, BD, USA, or E-tests – Liofilchem, IT. Carbapenemase detection was performed by multiplex real-time PCR according to the ESCMID 2019 protocol. RESULTS: From 720 Enterobacterales isolated, 5.5% were CRE: 35 Klebsiella pneumoniae and 1 of Escherichia coli, Enterobacter cloacae, Enterobacter xiangfangensis, Proteus mirabilis and Providentia stuartii each. All strains were polyresistant, occasionally susceptible to gentamicin, amikacin, co-trimoxazole or fosfomycin. Among carbapenem-resistant Klebsiellae (20%) 8 were colistin resistant. Two strains producing KPC were susceptible to newer antibiotics: ceftazidime-avibactam, imipenem-relebactam, meropenem-vaborbactam, cefiderocole, plazomicin, the remaining demonstrated different rate of susceptibility. Two isolates produced OXA–48 and the rest - metallo-β-lactamases - 31 NDM and 3 VIM type. Antibiotic activity was: cefiderocol > plazomicin > imipenem/relebactam > meropenem/vaborbactam > ceftazidime/avibactam with MIC50 of 0.21mg/L, 1mg/L, 4.5mg/L, 8.5mg/L and 12mg/L respectively. CONCLUSIONS: The high rate of carbapenemase producers with the prevalence of K.pneumoniae with the NDM is particularly worrying: some of the appropriate antibiotics are not available at present in the country. |
| format | Article |
| id | doaj-art-3b76cbbc8e6e4ecca14df38de8bc99cc |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-3b76cbbc8e6e4ecca14df38de8bc99cc2024-12-27T04:08:17ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-013911Antimicrobial susceptibility of clinically important carbapenem-resistant Enterobacterales towards standard and new antibioticsDeniz Hamidov0Emma Keuleyan1Theodor Todorov2Radoslava Vazharova3Georgi Todorov4Atanas Syarov5Laboratory of Clinical Microbiology and Virology, University hospital “Lozenetz”, SofiaLaboratory of Clinical Microbiology and Virology, University hospital “Lozenetz”, SofiaLaboratory of Medical Genetics and Molecular Biology, University hospital “Lozenetz”, SofiaMedical Faculty, Sofia University “St. Kliment Ochridski”Laboratory of Clinical Microbiology and Virology, University hospital “Lozenetz”, SofiaLaboratory of Medical Genetics and Molecular Biology, University hospital “Lozenetz”, SofiaAIM: The aim of this work is to study carbapenem-resistant Enterobacterales (CRE) incidence, carbapenemase types and antimicrobial susceptibility of the strains to standard and new antibiotics. BACKGROUND: CRE, carbapenemase–producers, are of critical importance (WHO, CDC), as determinants of difficult to treat infections. Their spread should be controlled and new effective antibiotics are urgently needed. METHODS: During 2023, CRE from patients of the University hospital “Lozenetz” – Sofia were isolated from different clinical specimens and identified by MALDI-tof, Bruker, DE. Susceptibility testing (EUCAST) included Disc diffusion method, MIC determination by Phoenix, BD, USA, or E-tests – Liofilchem, IT. Carbapenemase detection was performed by multiplex real-time PCR according to the ESCMID 2019 protocol. RESULTS: From 720 Enterobacterales isolated, 5.5% were CRE: 35 Klebsiella pneumoniae and 1 of Escherichia coli, Enterobacter cloacae, Enterobacter xiangfangensis, Proteus mirabilis and Providentia stuartii each. All strains were polyresistant, occasionally susceptible to gentamicin, amikacin, co-trimoxazole or fosfomycin. Among carbapenem-resistant Klebsiellae (20%) 8 were colistin resistant. Two strains producing KPC were susceptible to newer antibiotics: ceftazidime-avibactam, imipenem-relebactam, meropenem-vaborbactam, cefiderocole, plazomicin, the remaining demonstrated different rate of susceptibility. Two isolates produced OXA–48 and the rest - metallo-β-lactamases - 31 NDM and 3 VIM type. Antibiotic activity was: cefiderocol > plazomicin > imipenem/relebactam > meropenem/vaborbactam > ceftazidime/avibactam with MIC50 of 0.21mg/L, 1mg/L, 4.5mg/L, 8.5mg/L and 12mg/L respectively. CONCLUSIONS: The high rate of carbapenemase producers with the prevalence of K.pneumoniae with the NDM is particularly worrying: some of the appropriate antibiotics are not available at present in the country.http://www.sciencedirect.com/science/article/pii/S2213716524002108Carbapenemases-NDM-producers-susceptibility to newer antibiotics-Bulgaria |
| spellingShingle | Deniz Hamidov Emma Keuleyan Theodor Todorov Radoslava Vazharova Georgi Todorov Atanas Syarov Antimicrobial susceptibility of clinically important carbapenem-resistant Enterobacterales towards standard and new antibiotics Journal of Global Antimicrobial Resistance Carbapenemases- NDM-producers- susceptibility to newer antibiotics- Bulgaria |
| title | Antimicrobial susceptibility of clinically important carbapenem-resistant Enterobacterales towards standard and new antibiotics |
| title_full | Antimicrobial susceptibility of clinically important carbapenem-resistant Enterobacterales towards standard and new antibiotics |
| title_fullStr | Antimicrobial susceptibility of clinically important carbapenem-resistant Enterobacterales towards standard and new antibiotics |
| title_full_unstemmed | Antimicrobial susceptibility of clinically important carbapenem-resistant Enterobacterales towards standard and new antibiotics |
| title_short | Antimicrobial susceptibility of clinically important carbapenem-resistant Enterobacterales towards standard and new antibiotics |
| title_sort | antimicrobial susceptibility of clinically important carbapenem resistant enterobacterales towards standard and new antibiotics |
| topic | Carbapenemases- NDM-producers- susceptibility to newer antibiotics- Bulgaria |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524002108 |
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