Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery System

Background: Deer placenta extract (DPE), rich in bioactive macromolecules, promotes regenerative effects in both normal and damaged cells. However, effective delivery of these macromolecules through the skin remains a challenge. Objectives: To investigate the potential of DPE in regenerating hair ce...

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Main Authors: Worranan Rangsimawong, Sureewan Duangjit, Phaijit Sritananuwat, Tanasait Ngawhirunpat, Praneet Opanasopit
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Cosmetics
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Online Access:https://www.mdpi.com/2079-9284/11/6/204
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author Worranan Rangsimawong
Sureewan Duangjit
Phaijit Sritananuwat
Tanasait Ngawhirunpat
Praneet Opanasopit
author_facet Worranan Rangsimawong
Sureewan Duangjit
Phaijit Sritananuwat
Tanasait Ngawhirunpat
Praneet Opanasopit
author_sort Worranan Rangsimawong
collection DOAJ
description Background: Deer placenta extract (DPE), rich in bioactive macromolecules, promotes regenerative effects in both normal and damaged cells. However, effective delivery of these macromolecules through the skin remains a challenge. Objectives: To investigate the potential of DPE in regenerating hair cells and to develop a nanoniosome (NS) and microspicule (MS) formulation as a promising transfollicular delivery system. Methods: The bioactivity of DPE was assessed in human follicle dermal papilla (HFDP) cells, including cells damaged by chemotherapy. The NS-MS formulation was designed to deliver biomacromolecules from DPE into skin and hair follicles. Results: DPE at 2000 µg/mL exhibited no cytotoxicity and significantly enhanced proliferation in both normal and cisplatin-treated HFDP cells. The DPE-loaded NSs displayed nanovesicles with a uniform size distribution and a negative surface charge. When incorporated into the MS gel, NS-MS demonstrated a synergistic effect, significantly enhancing the transdermal and transfollicular delivery of macromolecular protein into the skin layers and hair follicles. Conclusions: DPE promoted hair cell proliferation and facilitated the recovery of hair cells damaged by chemotherapy, especially when formulated into the NS-MS system, which effectively delivered bioactive macromolecules to the target site. This suggests its potential role in promoting hair regrowth.
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institution Kabale University
issn 2079-9284
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publishDate 2024-11-01
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series Cosmetics
spelling doaj-art-3b406bf0de1f4df0b101c43002f2a5432024-12-27T14:19:21ZengMDPI AGCosmetics2079-92842024-11-0111620410.3390/cosmetics11060204Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery SystemWorranan Rangsimawong0Sureewan Duangjit1Phaijit Sritananuwat2Tanasait Ngawhirunpat3Praneet Opanasopit4Innovation in Drug and Extract of Agriculture Research Group, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, ThailandInnovation in Drug and Extract of Agriculture Research Group, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, ThailandInnovation in Drug and Extract of Agriculture Research Group, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, ThailandDepartment of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, ThailandDepartment of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, ThailandBackground: Deer placenta extract (DPE), rich in bioactive macromolecules, promotes regenerative effects in both normal and damaged cells. However, effective delivery of these macromolecules through the skin remains a challenge. Objectives: To investigate the potential of DPE in regenerating hair cells and to develop a nanoniosome (NS) and microspicule (MS) formulation as a promising transfollicular delivery system. Methods: The bioactivity of DPE was assessed in human follicle dermal papilla (HFDP) cells, including cells damaged by chemotherapy. The NS-MS formulation was designed to deliver biomacromolecules from DPE into skin and hair follicles. Results: DPE at 2000 µg/mL exhibited no cytotoxicity and significantly enhanced proliferation in both normal and cisplatin-treated HFDP cells. The DPE-loaded NSs displayed nanovesicles with a uniform size distribution and a negative surface charge. When incorporated into the MS gel, NS-MS demonstrated a synergistic effect, significantly enhancing the transdermal and transfollicular delivery of macromolecular protein into the skin layers and hair follicles. Conclusions: DPE promoted hair cell proliferation and facilitated the recovery of hair cells damaged by chemotherapy, especially when formulated into the NS-MS system, which effectively delivered bioactive macromolecules to the target site. This suggests its potential role in promoting hair regrowth.https://www.mdpi.com/2079-9284/11/6/204deer placenta extractbioactive macromoleculesdermal papilla cellschemotherapy-induced damage hair cellsniosomesmicrospicules
spellingShingle Worranan Rangsimawong
Sureewan Duangjit
Phaijit Sritananuwat
Tanasait Ngawhirunpat
Praneet Opanasopit
Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery System
Cosmetics
deer placenta extract
bioactive macromolecules
dermal papilla cells
chemotherapy-induced damage hair cells
niosomes
microspicules
title Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery System
title_full Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery System
title_fullStr Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery System
title_full_unstemmed Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery System
title_short Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery System
title_sort potential of deer placenta extract in hair cell regeneration and its nanoniosome microspicule gel as a transfollicular delivery system
topic deer placenta extract
bioactive macromolecules
dermal papilla cells
chemotherapy-induced damage hair cells
niosomes
microspicules
url https://www.mdpi.com/2079-9284/11/6/204
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