Immunogenic peptides putatively from intratumor microbes: Opportunities for colorectal cancer treatment
Summary: Recent evidence has confirmed the presence of intratumor microbes, yet their impact on the immunopeptidome remains largely unexplored. Here we introduced an integrated strategy to identify the immunopeptidome originated from intratumor microbes. Analyzing 10 colorectal cancer (CRC) patients...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422402563X |
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| author | Xiangyu Guan Fanyu Bu Yunyun Fu Haibo Zhang Haitao Xiang Xinle Chen Tai Chen Xiaojian Wu Kui Wu Longqi Liu Xuan Dong |
| author_facet | Xiangyu Guan Fanyu Bu Yunyun Fu Haibo Zhang Haitao Xiang Xinle Chen Tai Chen Xiaojian Wu Kui Wu Longqi Liu Xuan Dong |
| author_sort | Xiangyu Guan |
| collection | DOAJ |
| description | Summary: Recent evidence has confirmed the presence of intratumor microbes, yet their impact on the immunopeptidome remains largely unexplored. Here we introduced an integrated strategy to identify the immunopeptidome originated from intratumor microbes. Analyzing 10 colorectal cancer (CRC) patients, we identified 154 putative microbe-derived human leukocyte antigen (HLA)-I ligands. Predominantly bacterial in origin, these peptides were notably abundant in Fusobacterium nucleatum, the most prevalent bacterium differentiating between normal and tumor tissues. We discovered 20 peptides originating from F. nucleatum, thirteen of which, including two peptides shared across multiple patients, were tumor specific. Validation experiments confirmed that the putative microbe-derived peptide could activate CD8+ T cell responses. Our findings indicate that HLA-I molecules are capable of presenting intratumor microbe-derived peptides in CRC, potentially contributing to CD8+ T cell-mediated immunity and suggesting potential strategies for cancer immunotherapy. |
| format | Article |
| id | doaj-art-3b03a5958c3b4410a870c8eae7af66f3 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-3b03a5958c3b4410a870c8eae7af66f32024-12-22T05:28:59ZengElsevieriScience2589-00422024-12-012712111338Immunogenic peptides putatively from intratumor microbes: Opportunities for colorectal cancer treatmentXiangyu Guan0Fanyu Bu1Yunyun Fu2Haibo Zhang3Haitao Xiang4Xinle Chen5Tai Chen6Xiaojian Wu7Kui Wu8Longqi Liu9Xuan Dong10College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, ChinaBGI Research, Hangzhou 310030, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Hangzhou 310030, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Hangzhou 310030, ChinaBGI Research, Hangzhou 310030, ChinaBGI Research, Hangzhou 310030, China; Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, 710049, ChinaBGI Research, Changzhou 213299, ChinaThe Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510655, ChinaBGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, China; Guangdong Provincial Key Laboratory of Human Disease Genomics, Shenzhen Key Laboratory of Genomics, BGI Research, Shenzhen 518083, China; HIM-BGI Omics Center, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences (CAS), Hangzhou 310022, ChinaBGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, China; Corresponding authorBGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, China; Guangdong Provincial Key Laboratory of Human Disease Genomics, Shenzhen Key Laboratory of Genomics, BGI Research, Shenzhen 518083, China; HIM-BGI Omics Center, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences (CAS), Hangzhou 310022, China; Corresponding authorSummary: Recent evidence has confirmed the presence of intratumor microbes, yet their impact on the immunopeptidome remains largely unexplored. Here we introduced an integrated strategy to identify the immunopeptidome originated from intratumor microbes. Analyzing 10 colorectal cancer (CRC) patients, we identified 154 putative microbe-derived human leukocyte antigen (HLA)-I ligands. Predominantly bacterial in origin, these peptides were notably abundant in Fusobacterium nucleatum, the most prevalent bacterium differentiating between normal and tumor tissues. We discovered 20 peptides originating from F. nucleatum, thirteen of which, including two peptides shared across multiple patients, were tumor specific. Validation experiments confirmed that the putative microbe-derived peptide could activate CD8+ T cell responses. Our findings indicate that HLA-I molecules are capable of presenting intratumor microbe-derived peptides in CRC, potentially contributing to CD8+ T cell-mediated immunity and suggesting potential strategies for cancer immunotherapy.http://www.sciencedirect.com/science/article/pii/S258900422402563XImmune responseMicrobiomeCancer |
| spellingShingle | Xiangyu Guan Fanyu Bu Yunyun Fu Haibo Zhang Haitao Xiang Xinle Chen Tai Chen Xiaojian Wu Kui Wu Longqi Liu Xuan Dong Immunogenic peptides putatively from intratumor microbes: Opportunities for colorectal cancer treatment iScience Immune response Microbiome Cancer |
| title | Immunogenic peptides putatively from intratumor microbes: Opportunities for colorectal cancer treatment |
| title_full | Immunogenic peptides putatively from intratumor microbes: Opportunities for colorectal cancer treatment |
| title_fullStr | Immunogenic peptides putatively from intratumor microbes: Opportunities for colorectal cancer treatment |
| title_full_unstemmed | Immunogenic peptides putatively from intratumor microbes: Opportunities for colorectal cancer treatment |
| title_short | Immunogenic peptides putatively from intratumor microbes: Opportunities for colorectal cancer treatment |
| title_sort | immunogenic peptides putatively from intratumor microbes opportunities for colorectal cancer treatment |
| topic | Immune response Microbiome Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S258900422402563X |
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