The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates
<b>Aim:</b> In this study, it was aim to determine the rates of the effect of the lasting time from thawing-transfer until to transfer during transferring of cattle frozen embryos with ethylene glycol by direct transfer method on pregnancy rates.<p> <b>Materials and Methods:&...
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| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1029 |
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| author | Şükrü Dursun Mehmet Köse Mesut Kırbaş Bülent Bülbül Seyit Ümütlü |
| author_facet | Şükrü Dursun Mehmet Köse Mesut Kırbaş Bülent Bülbül Seyit Ümütlü |
| author_sort | Şükrü Dursun |
| collection | DOAJ |
| description | <b>Aim:</b> In this study, it was aim to determine the rates of the effect
of the lasting time from thawing-transfer until to transfer during
transferring of cattle frozen embryos with ethylene glycol by
direct transfer method on pregnancy rates.<p>
<b>Materials and Methods:</b> Recipients (n=56) were selected from
the cows whose estrus were synchronized with two times injections
of 0.150 mg d-cloprostenol 14 days intervals. Embryos
were transferred into recipients 7 days after the detection of recipients
estrus (estrus= day 0). After embryos were threated 25
seconds within 25oC water bath to thaved then they were transferred
to the recipients in minutes as follows; 0-5 (Group 0-5,
n=27), 6-10 (Group 6-10, n=16) or 11-25 (Group 11-25, n=13)
which all embryos were transferred into the uterine horn ipsilateral
to the corpus luteum. Pregnancy diagnosis were done on
day 28 used by ultrasound.<p>
<b>Results:</b> Pregnancy rates were determined in 59.3%, 50% and
23.1% within G 0-5, G 6-10 and G 11-25 groups, respectively.
Therefore, pregnancy rates were not statistically significant
(P>0.05) between G 0-5 & G 6-10 with G 6-10 & G 11-25 while it
was lower than the group of G 11-25 compared to the group of
G 0-5 (P<0.05).<p>
<b>Conclusions:</b> According to this study, frozen embryos by ethylene
glycol should be transferred into recipients in within 10
minutes after thawing is concluded. |
| format | Article |
| id | doaj-art-3aa1fceeb2f4454d8c89baf986c8f7ff |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-3aa1fceeb2f4454d8c89baf986c8f7ff2025-01-02T22:36:23ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-195330248521029The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy ratesŞükrü Dursun0Mehmet Köse1Mesut Kırbaş2Bülent Bülbül3Seyit Ümütlü4Bahri Dağdaş Uluslararası Tarımsal Araştırma Enstitüsü, Konya, TürkiyeDicle Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Diyarbakır, TürkiyeBahri Dağdaş Uluslararası Tarımsal Araştırma Enstitüsü, Konya, TürkiyeBahri Dağdaş Uluslararası Tarımsal Araştırma Enstitüsü, Konya, TürkiyeBahri Dağdaş Uluslararası Tarımsal Araştırma Enstitüsü, Konya, Türkiye<b>Aim:</b> In this study, it was aim to determine the rates of the effect of the lasting time from thawing-transfer until to transfer during transferring of cattle frozen embryos with ethylene glycol by direct transfer method on pregnancy rates.<p> <b>Materials and Methods:</b> Recipients (n=56) were selected from the cows whose estrus were synchronized with two times injections of 0.150 mg d-cloprostenol 14 days intervals. Embryos were transferred into recipients 7 days after the detection of recipients estrus (estrus= day 0). After embryos were threated 25 seconds within 25oC water bath to thaved then they were transferred to the recipients in minutes as follows; 0-5 (Group 0-5, n=27), 6-10 (Group 6-10, n=16) or 11-25 (Group 11-25, n=13) which all embryos were transferred into the uterine horn ipsilateral to the corpus luteum. Pregnancy diagnosis were done on day 28 used by ultrasound.<p> <b>Results:</b> Pregnancy rates were determined in 59.3%, 50% and 23.1% within G 0-5, G 6-10 and G 11-25 groups, respectively. Therefore, pregnancy rates were not statistically significant (P>0.05) between G 0-5 & G 6-10 with G 6-10 & G 11-25 while it was lower than the group of G 11-25 compared to the group of G 0-5 (P<0.05).<p> <b>Conclusions:</b> According to this study, frozen embryos by ethylene glycol should be transferred into recipients in within 10 minutes after thawing is concluded.http://eurasianjvetsci.org/pdf.php3?id=1029thawing-transfer intervalembryotransfercowspregnancy rate |
| spellingShingle | Şükrü Dursun Mehmet Köse Mesut Kırbaş Bülent Bülbül Seyit Ümütlü The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates Eurasian Journal of Veterinary Sciences thawing-transfer interval embryo transfer cows pregnancy rate |
| title | The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates |
| title_full | The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates |
| title_fullStr | The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates |
| title_full_unstemmed | The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates |
| title_short | The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates |
| title_sort | effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates |
| topic | thawing-transfer interval embryo transfer cows pregnancy rate |
| url | http://eurasianjvetsci.org/pdf.php3?id=1029 |
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