The effect of time between thawing and transfer of cryopreserved cattle embryos by ethylene glycol on pregnancy rates
<b>Aim:</b> In this study, it was aim to determine the rates of the effect of the lasting time from thawing-transfer until to transfer during transferring of cattle frozen embryos with ethylene glycol by direct transfer method on pregnancy rates.<p> <b>Materials and Methods:&...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1029 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <b>Aim:</b> In this study, it was aim to determine the rates of the effect
of the lasting time from thawing-transfer until to transfer during
transferring of cattle frozen embryos with ethylene glycol by
direct transfer method on pregnancy rates.<p>
<b>Materials and Methods:</b> Recipients (n=56) were selected from
the cows whose estrus were synchronized with two times injections
of 0.150 mg d-cloprostenol 14 days intervals. Embryos
were transferred into recipients 7 days after the detection of recipients
estrus (estrus= day 0). After embryos were threated 25
seconds within 25oC water bath to thaved then they were transferred
to the recipients in minutes as follows; 0-5 (Group 0-5,
n=27), 6-10 (Group 6-10, n=16) or 11-25 (Group 11-25, n=13)
which all embryos were transferred into the uterine horn ipsilateral
to the corpus luteum. Pregnancy diagnosis were done on
day 28 used by ultrasound.<p>
<b>Results:</b> Pregnancy rates were determined in 59.3%, 50% and
23.1% within G 0-5, G 6-10 and G 11-25 groups, respectively.
Therefore, pregnancy rates were not statistically significant
(P>0.05) between G 0-5 & G 6-10 with G 6-10 & G 11-25 while it
was lower than the group of G 11-25 compared to the group of
G 0-5 (P<0.05).<p>
<b>Conclusions:</b> According to this study, frozen embryos by ethylene
glycol should be transferred into recipients in within 10
minutes after thawing is concluded. |
|---|---|
| ISSN: | 1309-6958 2146-1953 |