Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission Tomography
Intraplaque angiogenesis is associated with the occurrence of atherosclerotic plaque rupture. Cardiovascular molecular imaging can be used for the detection of rupture-prone plaques. Imaging with radiolabeled bevacizumab, a monoclonal anti-vascular endothelial growth factor (VEGF)-A, can depict VEGF...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2013-06-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2012.00034 |
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| author | Reza Golestani Clark J. Zeebregts Anton G.T. Terwisscha van Scheltinga Marjolijn N. Lub-de Hooge Gooitzen M. van Dam Andor W.J.M. Glaudemans Rudi A.J.O. Dierckx René A. Tio Albert J.H. Suurmeijer Hendrikus H. Boersma Wouter B. Nagengast Riemer H.J.A. Slart |
| author_facet | Reza Golestani Clark J. Zeebregts Anton G.T. Terwisscha van Scheltinga Marjolijn N. Lub-de Hooge Gooitzen M. van Dam Andor W.J.M. Glaudemans Rudi A.J.O. Dierckx René A. Tio Albert J.H. Suurmeijer Hendrikus H. Boersma Wouter B. Nagengast Riemer H.J.A. Slart |
| author_sort | Reza Golestani |
| collection | DOAJ |
| description | Intraplaque angiogenesis is associated with the occurrence of atherosclerotic plaque rupture. Cardiovascular molecular imaging can be used for the detection of rupture-prone plaques. Imaging with radiolabeled bevacizumab, a monoclonal anti-vascular endothelial growth factor (VEGF)-A, can depict VEGF levels corresponding to the angiogenic status in tumors. We determined the feasibility of 89 Zr-bevacizumab imaging for the detection of VEGF in carotid endarterectomy (CEA) specimens. Five CEA specimens were coincubated with 89 Zr-bevacizumab and aspecific 111 In-labeled IgG to determine the specificity of bevacizumab accumulation. In 11 CEA specimens, 89 Zr-bevacizumab micro-positron emission tomography (PET) was performed following 2 hours of incubation. Specimens were cut in 4 mm wide segments and were stained for VEGF and CD68. In each segment, the mean percent incubation dose per gram of tissue (%Inc/g) and tissue to background ratio were determined. A 10-fold higher accumulation of 89 Zr-bevacizumab compared to 111 In-IgG uptake was demonstrated by gamma counting. The mean %Inc/g hot spot was 2.2 ± 0.9 with a hot spot to background ratio of 3.6 ± 0.8. There was a significant correlation between the segmental tissue to background uptake ratio and the VEGF score (ρ = .74, p < .001). It is feasible to detect VEGF tissue concentration within CEA specimens using 89 Zr-bevacizumab PET. 89 Zr-bevacizumab accumulation in plaques is specific and correlates with immunohistochemistry scores. |
| format | Article |
| id | doaj-art-3a71e6d585634956b9cacf6b04c855eb |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2013-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-3a71e6d585634956b9cacf6b04c855eb2025-01-03T00:10:41ZengSAGE PublishingMolecular Imaging1536-01212013-06-011210.2310/7290.2012.0003410.2310_7290.2012.00034Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission TomographyReza GolestaniClark J. ZeebregtsAnton G.T. Terwisscha van ScheltingaMarjolijn N. Lub-de HoogeGooitzen M. van DamAndor W.J.M. GlaudemansRudi A.J.O. DierckxRené A. TioAlbert J.H. SuurmeijerHendrikus H. BoersmaWouter B. NagengastRiemer H.J.A. SlartIntraplaque angiogenesis is associated with the occurrence of atherosclerotic plaque rupture. Cardiovascular molecular imaging can be used for the detection of rupture-prone plaques. Imaging with radiolabeled bevacizumab, a monoclonal anti-vascular endothelial growth factor (VEGF)-A, can depict VEGF levels corresponding to the angiogenic status in tumors. We determined the feasibility of 89 Zr-bevacizumab imaging for the detection of VEGF in carotid endarterectomy (CEA) specimens. Five CEA specimens were coincubated with 89 Zr-bevacizumab and aspecific 111 In-labeled IgG to determine the specificity of bevacizumab accumulation. In 11 CEA specimens, 89 Zr-bevacizumab micro-positron emission tomography (PET) was performed following 2 hours of incubation. Specimens were cut in 4 mm wide segments and were stained for VEGF and CD68. In each segment, the mean percent incubation dose per gram of tissue (%Inc/g) and tissue to background ratio were determined. A 10-fold higher accumulation of 89 Zr-bevacizumab compared to 111 In-IgG uptake was demonstrated by gamma counting. The mean %Inc/g hot spot was 2.2 ± 0.9 with a hot spot to background ratio of 3.6 ± 0.8. There was a significant correlation between the segmental tissue to background uptake ratio and the VEGF score (ρ = .74, p < .001). It is feasible to detect VEGF tissue concentration within CEA specimens using 89 Zr-bevacizumab PET. 89 Zr-bevacizumab accumulation in plaques is specific and correlates with immunohistochemistry scores.https://doi.org/10.2310/7290.2012.00034 |
| spellingShingle | Reza Golestani Clark J. Zeebregts Anton G.T. Terwisscha van Scheltinga Marjolijn N. Lub-de Hooge Gooitzen M. van Dam Andor W.J.M. Glaudemans Rudi A.J.O. Dierckx René A. Tio Albert J.H. Suurmeijer Hendrikus H. Boersma Wouter B. Nagengast Riemer H.J.A. Slart Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission Tomography Molecular Imaging |
| title | Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission Tomography |
| title_full | Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission Tomography |
| title_fullStr | Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission Tomography |
| title_full_unstemmed | Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission Tomography |
| title_short | Feasibility of Vascular Endothelial Growth Factor Imaging in Human Atherosclerotic Plaque Using Zr-Bevacizumab Positron Emission Tomography |
| title_sort | feasibility of vascular endothelial growth factor imaging in human atherosclerotic plaque using zr bevacizumab positron emission tomography |
| url | https://doi.org/10.2310/7290.2012.00034 |
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