Baseline microbiota of blueberries, soil, and irrigation water from blueberry farms located in three geographical regions
Bacterial microbiota was determined in fruit, soil, and irrigation water from blueberry (Vaccinium spp.) farms located in Cundinamarca, Colombia; Mississippi, United States; and Jalisco, Mexico. Bacterial communities were studied using 16S ribosomal ribonucleic acid (rRNA) gene amplification by targ...
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Elsevier
2024-12-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402416793X |
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| author | Angelica Abdallah-Ruiz Clara Esteban-Perez Shecoya B. White Wes Schilling Xue Zhang Eric T. Stafne Alejandro Rodríguez-Magaña Fernando Peña-Baracaldo Carlos A. Moreno-Ortiz Juan L. Silva |
| author_facet | Angelica Abdallah-Ruiz Clara Esteban-Perez Shecoya B. White Wes Schilling Xue Zhang Eric T. Stafne Alejandro Rodríguez-Magaña Fernando Peña-Baracaldo Carlos A. Moreno-Ortiz Juan L. Silva |
| author_sort | Angelica Abdallah-Ruiz |
| collection | DOAJ |
| description | Bacterial microbiota was determined in fruit, soil, and irrigation water from blueberry (Vaccinium spp.) farms located in Cundinamarca, Colombia; Mississippi, United States; and Jalisco, Mexico. Bacterial communities were studied using 16S ribosomal ribonucleic acid (rRNA) gene amplification by targeting the V3–V4 hypervariable region. The most abundant phylum in fruit was Proteobacteria in Colombia and the United States and Firmicutes in Mexico. The most abundant phylum in soil and water was Proteobacteria for all regions. The top three genera found in fruit were Heliorestis (9.2 %), Rhodanobacter (3.3 %), and Sphingomonas (2.8 %) for Colombia, Heliorestis (23.1 %), Thiomonas (8.5 %), and Methylobacterium (3.3 %) for the United States, and Heliorestis (47.4 %), Thiomonas (9.1 %), and Bacillus (4.6 %) for Mexico. Colombia reported the highest (Padj < 0.05) alpha diversity for blueberries, and United States and Mexico had similar (Padj > 0.05) results. Beta diversity revealed bacterial communities in fruit differed (P < 0.05) by region. Bacterial differences existed between Colombia, United States, and Mexico for soil and fruit (P = 0.021, 0.003, and 0.006, respectively) and water and fruit (P = 0.003, 0.003, and 0.033, respectively). Blueberries grown in the three different regions have unique microbiota. Fruit and fruit-environment microbial composition also differed by region. These results provide a more complete profile of the bacterial communities on blueberries and their agricultural environments and could contribute to better management and decision-making practices in terms of plant health, food quality, and food safety. |
| format | Article |
| id | doaj-art-3a5f579e671747f4ab65bb076deda35c |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-3a5f579e671747f4ab65bb076deda35c2024-12-13T10:59:44ZengElsevierHeliyon2405-84402024-12-011023e40762Baseline microbiota of blueberries, soil, and irrigation water from blueberry farms located in three geographical regionsAngelica Abdallah-Ruiz0Clara Esteban-Perez1Shecoya B. White2Wes Schilling3Xue Zhang4Eric T. Stafne5Alejandro Rodríguez-Magaña6Fernando Peña-Baracaldo7Carlos A. Moreno-Ortiz8Juan L. Silva9Department of Biochemistry, Nutrition, and Health Promotion, Mississippi State University, Mississippi State, MS, 39762, USA; Corresponding author.Sequencing Department, Invitrocell SAS, Bogotá, 110111, ColombiaDepartment of Biochemistry, Nutrition, and Health Promotion, Mississippi State University, Mississippi State, MS, 39762, USADepartment of Biochemistry, Nutrition, and Health Promotion, Mississippi State University, Mississippi State, MS, 39762, USADepartment of Biochemistry, Nutrition, and Health Promotion, Mississippi State University, Mississippi State, MS, 39762, USASouth Branch Experiment Station, Coastal Research and Extension Center, Mississippi State University, Poplarville, MS, 39470, USAFacultad de Ciencias Económicas y Empresariales, Universidad Panamericana de Guadalajara, Guadalajara, 45010, MexicoFacultad de Ciencias Agropecuarias, Universidad de Ciencias Aplicadas y Ambientales U.D.C.A., Bogotá, 111166, ColombiaFacultad de Ciencias Administrativas y Comerciales, Universidad de Ciencias Aplicadas y Ambientales U.D.C.A., Bogotá, 111166, ColombiaDepartment of Biochemistry, Nutrition, and Health Promotion, Mississippi State University, Mississippi State, MS, 39762, USABacterial microbiota was determined in fruit, soil, and irrigation water from blueberry (Vaccinium spp.) farms located in Cundinamarca, Colombia; Mississippi, United States; and Jalisco, Mexico. Bacterial communities were studied using 16S ribosomal ribonucleic acid (rRNA) gene amplification by targeting the V3–V4 hypervariable region. The most abundant phylum in fruit was Proteobacteria in Colombia and the United States and Firmicutes in Mexico. The most abundant phylum in soil and water was Proteobacteria for all regions. The top three genera found in fruit were Heliorestis (9.2 %), Rhodanobacter (3.3 %), and Sphingomonas (2.8 %) for Colombia, Heliorestis (23.1 %), Thiomonas (8.5 %), and Methylobacterium (3.3 %) for the United States, and Heliorestis (47.4 %), Thiomonas (9.1 %), and Bacillus (4.6 %) for Mexico. Colombia reported the highest (Padj < 0.05) alpha diversity for blueberries, and United States and Mexico had similar (Padj > 0.05) results. Beta diversity revealed bacterial communities in fruit differed (P < 0.05) by region. Bacterial differences existed between Colombia, United States, and Mexico for soil and fruit (P = 0.021, 0.003, and 0.006, respectively) and water and fruit (P = 0.003, 0.003, and 0.033, respectively). Blueberries grown in the three different regions have unique microbiota. Fruit and fruit-environment microbial composition also differed by region. These results provide a more complete profile of the bacterial communities on blueberries and their agricultural environments and could contribute to better management and decision-making practices in terms of plant health, food quality, and food safety.http://www.sciencedirect.com/science/article/pii/S240584402416793X16S rRNA gene sequencingBacterial microbiotaBlueberriesSoilIrrigation water |
| spellingShingle | Angelica Abdallah-Ruiz Clara Esteban-Perez Shecoya B. White Wes Schilling Xue Zhang Eric T. Stafne Alejandro Rodríguez-Magaña Fernando Peña-Baracaldo Carlos A. Moreno-Ortiz Juan L. Silva Baseline microbiota of blueberries, soil, and irrigation water from blueberry farms located in three geographical regions Heliyon 16S rRNA gene sequencing Bacterial microbiota Blueberries Soil Irrigation water |
| title | Baseline microbiota of blueberries, soil, and irrigation water from blueberry farms located in three geographical regions |
| title_full | Baseline microbiota of blueberries, soil, and irrigation water from blueberry farms located in three geographical regions |
| title_fullStr | Baseline microbiota of blueberries, soil, and irrigation water from blueberry farms located in three geographical regions |
| title_full_unstemmed | Baseline microbiota of blueberries, soil, and irrigation water from blueberry farms located in three geographical regions |
| title_short | Baseline microbiota of blueberries, soil, and irrigation water from blueberry farms located in three geographical regions |
| title_sort | baseline microbiota of blueberries soil and irrigation water from blueberry farms located in three geographical regions |
| topic | 16S rRNA gene sequencing Bacterial microbiota Blueberries Soil Irrigation water |
| url | http://www.sciencedirect.com/science/article/pii/S240584402416793X |
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