miR-199a-3p靶向视网膜母细胞瘤转录辅阻遏子1促进心肌细胞肥大
【目的】探讨微小RNAmicroRNA-199a-3p（miR-199a-3p）对心肌细胞肥大的调控作用及其作用靶基因。【方法】原代分离培养C57BL/6乳小鼠心肌细胞（NMVC），转染miR-199a-3p模拟物（mimic）和视网膜母细胞瘤转录辅阻遏子1（Rb-1）siRNA分别来增加NMVC中miR-199a-3p和抑制Rb-1的水平。双荧光素酶报告基因实验检测miR-199a-3p与潜在靶基因Rb-13′端非翻译区（3′-UTR）的结合作用。FITC-鬼笔环肽染色检测乳小鼠心肌细胞表面积。RT-qPCR和Westernblot检测miR-199a-3p，Rb-1和心肌细胞肥大相关基因的...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2019-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43546206/ |
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