Unraveling anti-inflammatory components and mechanisms in decoction derived from Allium ascalonicum L. Bulbs and Sojae Semen Praeparatum via UPLC-MS/MS integrated with bioinformatics
The Cong-Chi decoction (CCD) is composed of Allium ascalonicum L. bulbs and Sojae Semen Praeparatum. Food-based therapy exhibits minimal side effects when compared to the conventional use of antipyretic and analgesic drugs for treating common cold (wind-cold type). However, the current state of rese...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
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| Series: | CyTA - Journal of Food |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2024.2363927 |
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| Summary: | The Cong-Chi decoction (CCD) is composed of Allium ascalonicum L. bulbs and Sojae Semen Praeparatum. Food-based therapy exhibits minimal side effects when compared to the conventional use of antipyretic and analgesic drugs for treating common cold (wind-cold type). However, the current state of research is deficient in exploring the components of CCD, and there is a lack of assessment regarding the fever-reducing and anti-inflammatory mechanisms associated with CCD. This study synergizes Ultra-Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (UPLC-MS/MS) with bioinformatics, validating the anti-inflammatory components of CCD and predicting its anti-inflammatory mechanisms. Our results reveal that UPLC-MS/MS identified 33 components in CCD. Notably, components such as genistein, glycitein, daidzein, scopoletin, and 7-hydroxycoumarin demonstrated a high binding affinity with the core protein Tumor Necrosis Factor-alpha (TNF-α). They inhibited the expression of TNF-α and Interleukin-6 (IL-6) in a Lipopolysaccharide (LPS)-induced inflammation model. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicates that anti-inflammatory mechanisms of CCD involve pathways related to cell apoptosis, arginine and proline metabolism, and Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling. |
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| ISSN: | 1947-6337 1947-6345 |