Formalin-free tissue embedding is less hazardous and results in better DNA quality.
Disease diagnosis, prognosis, and therapy choice progressively rely on good-quality Deoxyribonucleic acid (DNA) for molecular analysis. As tissue processing is routinely performed worldwide with ancient techniques using toxic and DNA-damaging formaldehyde, the quality of DNA isolated from embedded t...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0316107 |
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| _version_ | 1841533076343095296 |
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| author | Maarten Niemantsverdriet Ageeth Knol Jose van der Starre-Gaal Agnes Marije Hoogland |
| author_facet | Maarten Niemantsverdriet Ageeth Knol Jose van der Starre-Gaal Agnes Marije Hoogland |
| author_sort | Maarten Niemantsverdriet |
| collection | DOAJ |
| description | Disease diagnosis, prognosis, and therapy choice progressively rely on good-quality Deoxyribonucleic acid (DNA) for molecular analysis. As tissue processing is routinely performed worldwide with ancient techniques using toxic and DNA-damaging formaldehyde, the quality of DNA isolated from embedded tissues used for diagnostics is poor. We used a novel formalin-free tissue embedding method to process tissues and show that, after 5 years, DNA quality is superior to formalin fixation. |
| format | Article |
| id | doaj-art-391766a5d3444f518a612971ecbde2a0 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-391766a5d3444f518a612971ecbde2a02025-01-17T05:31:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031610710.1371/journal.pone.0316107Formalin-free tissue embedding is less hazardous and results in better DNA quality.Maarten NiemantsverdrietAgeeth KnolJose van der Starre-GaalAgnes Marije HooglandDisease diagnosis, prognosis, and therapy choice progressively rely on good-quality Deoxyribonucleic acid (DNA) for molecular analysis. As tissue processing is routinely performed worldwide with ancient techniques using toxic and DNA-damaging formaldehyde, the quality of DNA isolated from embedded tissues used for diagnostics is poor. We used a novel formalin-free tissue embedding method to process tissues and show that, after 5 years, DNA quality is superior to formalin fixation.https://doi.org/10.1371/journal.pone.0316107 |
| spellingShingle | Maarten Niemantsverdriet Ageeth Knol Jose van der Starre-Gaal Agnes Marije Hoogland Formalin-free tissue embedding is less hazardous and results in better DNA quality. PLoS ONE |
| title | Formalin-free tissue embedding is less hazardous and results in better DNA quality. |
| title_full | Formalin-free tissue embedding is less hazardous and results in better DNA quality. |
| title_fullStr | Formalin-free tissue embedding is less hazardous and results in better DNA quality. |
| title_full_unstemmed | Formalin-free tissue embedding is less hazardous and results in better DNA quality. |
| title_short | Formalin-free tissue embedding is less hazardous and results in better DNA quality. |
| title_sort | formalin free tissue embedding is less hazardous and results in better dna quality |
| url | https://doi.org/10.1371/journal.pone.0316107 |
| work_keys_str_mv | AT maartenniemantsverdriet formalinfreetissueembeddingislesshazardousandresultsinbetterdnaquality AT ageethknol formalinfreetissueembeddingislesshazardousandresultsinbetterdnaquality AT josevanderstarregaal formalinfreetissueembeddingislesshazardousandresultsinbetterdnaquality AT agnesmarijehoogland formalinfreetissueembeddingislesshazardousandresultsinbetterdnaquality |