Phenotypic and Genotypic Characterization of Multi-Drug Resistant Mycobacterium tuberculosis
This study characterized multi-drug resistant M. tuberculosis phenotypically by LJ-proportion method and genotypically by Geno Type MTBDR plus LPA. Out of the forty M. tuberculosis isolates tested, two (5.0%) were found to be multi-drug resistant by LJ proportion method and one (2.5%) was MDR by LP...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Umaru Musa Yar'adua University, Katsina, Nigeria
2020-06-01
|
| Series: | UMYU Journal of Microbiology Research |
| Subjects: | |
| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/112 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846150666237509632 |
|---|---|
| author | Aliyu, M. S. Garba, I. Tijjani, M. B., Doko, M. H .I. Mukhtar G. L. Musa, B. Madika, A. |
| author_facet | Aliyu, M. S. Garba, I. Tijjani, M. B., Doko, M. H .I. Mukhtar G. L. Musa, B. Madika, A. |
| author_sort | Aliyu, M. S. |
| collection | DOAJ |
| description |
This study characterized multi-drug resistant M. tuberculosis phenotypically by LJ-proportion method and genotypically by Geno Type MTBDR plus LPA. Out of the forty M. tuberculosis isolates tested, two (5.0%) were found to be multi-drug resistant by LJ proportion method and one (2.5%) was MDR by LPA. None was found to be mono-resistant to any of the drugs by LJ however, one isolate was mono resistant to RIF and one was mono resistant to INH by LPA. Comparison of Geno Type MTBDRplus LPA and phenotypic LJ-proportion methods showed that one isolate was mono resistant to RIF and one was mono resistant to INH by LPA, one and two MDR-TB isolates respectively were characterized by genotypic and phenotypic methods. The remaining isolates were found to be pan susceptible by both methods. One isolate was characterized as MDR with bands at rpoβ MUT2A region and ihnA MUT2 corresponding to H526Y and A16G mutations respectively. Rifampicin mono resistance with band at rpoβ MUT3 corresponding to S531L was found in one isolate. Also, isoniazid mono resistance was observed in one isolate with ihnA MUT2 band corresponding to A16G mutation. This study has shown an overall high prevalence of MDR-TB in the study area which needs to be urgently addressed. Laboratory facilities for rapid drug resistance detection are needed across the country for early and accurate diagnosis of TB and drug resistant cases. This remains an important step in managing TB drug resistance in Nigeria.
|
| format | Article |
| id | doaj-art-38d04f31b5794ec4b51b49a475c017ad |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-38d04f31b5794ec4b51b49a475c017ad2024-11-28T11:07:46ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222020-06-015110.47430/ujmr.2051.004112Phenotypic and Genotypic Characterization of Multi-Drug Resistant Mycobacterium tuberculosisAliyu, M. S.0Garba, I.1Tijjani, M. B.,2Doko, M. H .I.3Mukhtar G. L.4Musa, B.5Madika, A.6Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria Department of Medical Microbiology, Faculty of Medical Laboratory Science, Usmanu Danfodiyo University, SokotoDepartment of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria Department of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University Katsina, Katsina State, Nigeria.Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, ZariaDepartment of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria This study characterized multi-drug resistant M. tuberculosis phenotypically by LJ-proportion method and genotypically by Geno Type MTBDR plus LPA. Out of the forty M. tuberculosis isolates tested, two (5.0%) were found to be multi-drug resistant by LJ proportion method and one (2.5%) was MDR by LPA. None was found to be mono-resistant to any of the drugs by LJ however, one isolate was mono resistant to RIF and one was mono resistant to INH by LPA. Comparison of Geno Type MTBDRplus LPA and phenotypic LJ-proportion methods showed that one isolate was mono resistant to RIF and one was mono resistant to INH by LPA, one and two MDR-TB isolates respectively were characterized by genotypic and phenotypic methods. The remaining isolates were found to be pan susceptible by both methods. One isolate was characterized as MDR with bands at rpoβ MUT2A region and ihnA MUT2 corresponding to H526Y and A16G mutations respectively. Rifampicin mono resistance with band at rpoβ MUT3 corresponding to S531L was found in one isolate. Also, isoniazid mono resistance was observed in one isolate with ihnA MUT2 band corresponding to A16G mutation. This study has shown an overall high prevalence of MDR-TB in the study area which needs to be urgently addressed. Laboratory facilities for rapid drug resistance detection are needed across the country for early and accurate diagnosis of TB and drug resistant cases. This remains an important step in managing TB drug resistance in Nigeria. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/112Multi-drug resistance,M. tuberculosis,phenotypic,genotypic, LJ-proportion,Geno Type MTBDRplusLPA, |
| spellingShingle | Aliyu, M. S. Garba, I. Tijjani, M. B., Doko, M. H .I. Mukhtar G. L. Musa, B. Madika, A. Phenotypic and Genotypic Characterization of Multi-Drug Resistant Mycobacterium tuberculosis UMYU Journal of Microbiology Research Multi-drug resistance, M. tuberculosis, phenotypic, genotypic, LJ-proportion, Geno Type MTBDRplus LPA, |
| title | Phenotypic and Genotypic Characterization of Multi-Drug Resistant Mycobacterium tuberculosis |
| title_full | Phenotypic and Genotypic Characterization of Multi-Drug Resistant Mycobacterium tuberculosis |
| title_fullStr | Phenotypic and Genotypic Characterization of Multi-Drug Resistant Mycobacterium tuberculosis |
| title_full_unstemmed | Phenotypic and Genotypic Characterization of Multi-Drug Resistant Mycobacterium tuberculosis |
| title_short | Phenotypic and Genotypic Characterization of Multi-Drug Resistant Mycobacterium tuberculosis |
| title_sort | phenotypic and genotypic characterization of multi drug resistant mycobacterium tuberculosis |
| topic | Multi-drug resistance, M. tuberculosis, phenotypic, genotypic, LJ-proportion, Geno Type MTBDRplus LPA, |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/112 |
| work_keys_str_mv | AT aliyums phenotypicandgenotypiccharacterizationofmultidrugresistantmycobacteriumtuberculosis AT garbai phenotypicandgenotypiccharacterizationofmultidrugresistantmycobacteriumtuberculosis AT tijjanimb phenotypicandgenotypiccharacterizationofmultidrugresistantmycobacteriumtuberculosis AT dokomhi phenotypicandgenotypiccharacterizationofmultidrugresistantmycobacteriumtuberculosis AT mukhtargl phenotypicandgenotypiccharacterizationofmultidrugresistantmycobacteriumtuberculosis AT musab phenotypicandgenotypiccharacterizationofmultidrugresistantmycobacteriumtuberculosis AT madikaa phenotypicandgenotypiccharacterizationofmultidrugresistantmycobacteriumtuberculosis |