GYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKIN

The perspective of utilization of in vitro culture of unpollinated ovules for development of dihaploid lines of pumpkin is described in the article. The ovules of five hybrids were cultivated on CBM medium. The following combinations of growth regulators for gynogenesis induction were used: a) 0.2 m...

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Main Authors: N.A. Shmykova, D.V. Shumilina, V.P. Kushnereva, G.A. Khimich
Format: Article
Language:English
Published: Federal State Budgetary Scientific Institution "Federal Scientific Vegetable Center" 2011-03-01
Series:Овощи России
Subjects:
Online Access:https://www.vegetables.su/jour/article/view/1595
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author N.A. Shmykova
D.V. Shumilina
V.P. Kushnereva
G.A. Khimich
author_facet N.A. Shmykova
D.V. Shumilina
V.P. Kushnereva
G.A. Khimich
author_sort N.A. Shmykova
collection DOAJ
description The perspective of utilization of in vitro culture of unpollinated ovules for development of dihaploid lines of pumpkin is described in the article. The ovules of five hybrids were cultivated on CBM medium. The following combinations of growth regulators for gynogenesis induction were used: a) 0.2 mg/L of thidiazuron and 0,0001 мкМ of epibrassinolide; b) 2 mg/l of 2,4-D. The frequency of embryo development depended on cultivation medium and genotype. The maximum amount of embryos developed from unpollinated ovules of one ovary was 6-9 ones.
format Article
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institution Kabale University
issn 2072-9146
2618-7132
language English
publishDate 2011-03-01
publisher Federal State Budgetary Scientific Institution "Federal Scientific Vegetable Center"
record_format Article
series Овощи России
spelling doaj-art-38c3caa04f3f40aa9787c09d03b3956c2025-08-20T03:43:16ZengFederal State Budgetary Scientific Institution "Federal Scientific Vegetable Center"Овощи России2072-91462618-71322011-03-0101283110.18619/2072-9146-2011-1-28-311107GYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKINN.A. Shmykova0D.V. Shumilina1V.P. Kushnereva2G.A. Khimich3State Scientific Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production Russian Academy of Agricultural ScienceState Scientific Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production Russian Academy of Agricultural ScienceState Scientific Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production Russian Academy of Agricultural ScienceState Scientific Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production Russian Academy of Agricultural ScienceThe perspective of utilization of in vitro culture of unpollinated ovules for development of dihaploid lines of pumpkin is described in the article. The ovules of five hybrids were cultivated on CBM medium. The following combinations of growth regulators for gynogenesis induction were used: a) 0.2 mg/L of thidiazuron and 0,0001 мкМ of epibrassinolide; b) 2 mg/l of 2,4-D. The frequency of embryo development depended on cultivation medium and genotype. The maximum amount of embryos developed from unpollinated ovules of one ovary was 6-9 ones.https://www.vegetables.su/jour/article/view/1595culture of unpollinated ovulesgynogenesishaploid plants of pumpkinкультура неопыленных семяпочекгиногенезгаплоиды тыквы
spellingShingle N.A. Shmykova
D.V. Shumilina
V.P. Kushnereva
G.A. Khimich
GYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKIN
Овощи России
culture of unpollinated ovules
gynogenesis
haploid plants of pumpkin
культура неопыленных семяпочек
гиногенез
гаплоиды тыквы
title GYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKIN
title_full GYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKIN
title_fullStr GYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKIN
title_full_unstemmed GYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKIN
title_short GYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKIN
title_sort gynogenesis induction in culture of unpollinated ovules of pumpkin
topic culture of unpollinated ovules
gynogenesis
haploid plants of pumpkin
культура неопыленных семяпочек
гиногенез
гаплоиды тыквы
url https://www.vegetables.su/jour/article/view/1595
work_keys_str_mv AT nashmykova gynogenesisinductionincultureofunpollinatedovulesofpumpkin
AT dvshumilina gynogenesisinductionincultureofunpollinatedovulesofpumpkin
AT vpkushnereva gynogenesisinductionincultureofunpollinatedovulesofpumpkin
AT gakhimich gynogenesisinductionincultureofunpollinatedovulesofpumpkin