Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion?
Destabilization of heme proteins is recognized to play a role in acute kidney injury (AKI). Hemopexin (Hpx), known for its role in binding heme, mitigates free heme toxicity. Despite this, the potential adverse effects of Hpx deposition in kidney tissues and its impact on kidney function are not ful...
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MDPI AG
2024-11-01
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| Online Access: | https://www.mdpi.com/2218-273X/14/12/1522 |
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| author | You Hyun Jeon Eun-Joo Oh Se-Hyun Oh Jeong-Hoon Lim Hee-Yeon Jung Ji-Young Choi Jang-Hee Cho Sun-Hee Park Yong-Lim Kim Chan-Duck Kim |
| author_facet | You Hyun Jeon Eun-Joo Oh Se-Hyun Oh Jeong-Hoon Lim Hee-Yeon Jung Ji-Young Choi Jang-Hee Cho Sun-Hee Park Yong-Lim Kim Chan-Duck Kim |
| author_sort | You Hyun Jeon |
| collection | DOAJ |
| description | Destabilization of heme proteins is recognized to play a role in acute kidney injury (AKI). Hemopexin (Hpx), known for its role in binding heme, mitigates free heme toxicity. Despite this, the potential adverse effects of Hpx deposition in kidney tissues and its impact on kidney function are not fully understood. Deferoxamine (DFO) chelates iron released from heme and mitigates associated kidney damage. Therefore, this study aimed to evaluate whether Hpx contributes to kidney injury in an ischemia-reperfusion injury (IRI) induced AKI model and to investigate if DFO could alleviate this damage. Mice were categorized into five groups: Sham-Vehicle, Sham-Hpx, IRI-Vehicle, IRI-Hpx, and IRI-Hpx-DFO. Decline in kidney function was observed exclusively in the IRI group, independent of Hpx injection. Serum Hpx levels remained comparable across all groups, and administration of Hpx did not alter serum Hpx levels or kidney function after 24 hours. Although increased Hpx deposition in kidneys was noted in both the IRI and Hpx groups, this accumulation did not correlate with impaired kidney function. Additionally, DFO did not exhibit a protective effect against kidney injury. In summary, Hpx does not directly induce kidney injury and cannot be considered a biomarker for kidney damage caused by IRI. |
| format | Article |
| id | doaj-art-3878b4ada6cd41198d800b2d727ad89c |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-3878b4ada6cd41198d800b2d727ad89c2024-12-27T14:13:41ZengMDPI AGBiomolecules2218-273X2024-11-011412152210.3390/biom14121522Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion?You Hyun Jeon0Eun-Joo Oh1Se-Hyun Oh2Jeong-Hoon Lim3Hee-Yeon Jung4Ji-Young Choi5Jang-Hee Cho6Sun-Hee Park7Yong-Lim Kim8Chan-Duck Kim9Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDivision of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University Hospital, Kyungpook National University, Daegu 41944, Republic of KoreaDestabilization of heme proteins is recognized to play a role in acute kidney injury (AKI). Hemopexin (Hpx), known for its role in binding heme, mitigates free heme toxicity. Despite this, the potential adverse effects of Hpx deposition in kidney tissues and its impact on kidney function are not fully understood. Deferoxamine (DFO) chelates iron released from heme and mitigates associated kidney damage. Therefore, this study aimed to evaluate whether Hpx contributes to kidney injury in an ischemia-reperfusion injury (IRI) induced AKI model and to investigate if DFO could alleviate this damage. Mice were categorized into five groups: Sham-Vehicle, Sham-Hpx, IRI-Vehicle, IRI-Hpx, and IRI-Hpx-DFO. Decline in kidney function was observed exclusively in the IRI group, independent of Hpx injection. Serum Hpx levels remained comparable across all groups, and administration of Hpx did not alter serum Hpx levels or kidney function after 24 hours. Although increased Hpx deposition in kidneys was noted in both the IRI and Hpx groups, this accumulation did not correlate with impaired kidney function. Additionally, DFO did not exhibit a protective effect against kidney injury. In summary, Hpx does not directly induce kidney injury and cannot be considered a biomarker for kidney damage caused by IRI.https://www.mdpi.com/2218-273X/14/12/1522acute kidney injurybiomarkerhemopexinischemia-reperfusion injury |
| spellingShingle | You Hyun Jeon Eun-Joo Oh Se-Hyun Oh Jeong-Hoon Lim Hee-Yeon Jung Ji-Young Choi Jang-Hee Cho Sun-Hee Park Yong-Lim Kim Chan-Duck Kim Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion? Biomolecules acute kidney injury biomarker hemopexin ischemia-reperfusion injury |
| title | Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion? |
| title_full | Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion? |
| title_fullStr | Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion? |
| title_full_unstemmed | Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion? |
| title_short | Is Hemopexin a Nephrotoxin or a Marker of Kidney Injury in Renal Ischemia-Reperfusion? |
| title_sort | is hemopexin a nephrotoxin or a marker of kidney injury in renal ischemia reperfusion |
| topic | acute kidney injury biomarker hemopexin ischemia-reperfusion injury |
| url | https://www.mdpi.com/2218-273X/14/12/1522 |
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