NK cell exhaustion in Wilson’s disease revealed by single-cell RNA sequencing predicts the prognosis of cholecystitis
Metabolic abnormalities associated with liver disease have a significant impact on the risk and prognosis of cholecystitis. However, the underlying mechanism remains to be elucidated. Here, we investigated this issue using Wilson’s disease (WD) as a model, which is a genetic disorder characterized b...
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eLife Sciences Publications Ltd
2024-12-01
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| Online Access: | https://elifesciences.org/articles/98867 |
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| author | Yong Jin Jiayu Xing Chenyu Dai Lei Jin Wanying Zhang Qianqian Tao Mei Hou Ziyi Li Wen Yang Qiyu Feng Hongyang Wang Qingsheng Yu |
| author_facet | Yong Jin Jiayu Xing Chenyu Dai Lei Jin Wanying Zhang Qianqian Tao Mei Hou Ziyi Li Wen Yang Qiyu Feng Hongyang Wang Qingsheng Yu |
| author_sort | Yong Jin |
| collection | DOAJ |
| description | Metabolic abnormalities associated with liver disease have a significant impact on the risk and prognosis of cholecystitis. However, the underlying mechanism remains to be elucidated. Here, we investigated this issue using Wilson’s disease (WD) as a model, which is a genetic disorder characterized by impaired mitochondrial function and copper metabolism. Our retrospective clinical study found that WD patients have a significantly higher incidence of cholecystitis and a poorer prognosis. The hepatic immune cell landscape using single-cell RNA sequencing showed that the tissue immune microenvironment is altered in WD, mainly a major change in the constitution and function of the innate immune system. Exhaustion of natural killer (NK) cells is the fundamental factor, supported by the upregulated expression of inhibitory receptors and the downregulated expression of cytotoxic molecules, which was verified in clinical samples. Further bioinformatic analysis confirmed a positive correlation between NK cell exhaustion and poor prognosis in cholecystitis and other inflammatory diseases. The study demonstrated dysfunction of liver immune cells triggered by specific metabolic abnormalities in WD, with a focus on the correlation between NK cell exhaustion and poor healing of cholecystitis, providing new insights into the improvement of inflammatory diseases by assessing immune cell function. |
| format | Article |
| id | doaj-art-383f60c9c6d746c1a13c02b085756ea8 |
| institution | Kabale University |
| issn | 2050-084X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-383f60c9c6d746c1a13c02b085756ea82024-12-30T16:08:00ZengeLife Sciences Publications LtdeLife2050-084X2024-12-011310.7554/eLife.98867NK cell exhaustion in Wilson’s disease revealed by single-cell RNA sequencing predicts the prognosis of cholecystitisYong Jin0Jiayu Xing1Chenyu Dai2Lei Jin3Wanying Zhang4Qianqian Tao5Mei Hou6Ziyi Li7Wen Yang8Qiyu Feng9https://orcid.org/0009-0004-5032-7500Hongyang Wang10Qingsheng Yu11https://orcid.org/0000-0002-8074-0329Cancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, ChinaDepartment of Cadre Cardiology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, ChinaDepartment of General Surgery, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China; Institute of Chinese Medicine Surgery, Anhui Academy of Chinese Medicine, Hefei, ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, ChinaDepartment of General Surgery, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China; Institute of Chinese Medicine Surgery, Anhui Academy of Chinese Medicine, Hefei, ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, ChinaDepartment of General Surgery, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China; Institute of Chinese Medicine Surgery, Anhui Academy of Chinese Medicine, Hefei, ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China; National Center for Liver Cancer, Second Military Medical University, Shanghai, ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, ChinaCancer Research Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China; National Center for Liver Cancer, Second Military Medical University, Shanghai, ChinaDepartment of Cadre Cardiology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China; Department of General Surgery, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, ChinaMetabolic abnormalities associated with liver disease have a significant impact on the risk and prognosis of cholecystitis. However, the underlying mechanism remains to be elucidated. Here, we investigated this issue using Wilson’s disease (WD) as a model, which is a genetic disorder characterized by impaired mitochondrial function and copper metabolism. Our retrospective clinical study found that WD patients have a significantly higher incidence of cholecystitis and a poorer prognosis. The hepatic immune cell landscape using single-cell RNA sequencing showed that the tissue immune microenvironment is altered in WD, mainly a major change in the constitution and function of the innate immune system. Exhaustion of natural killer (NK) cells is the fundamental factor, supported by the upregulated expression of inhibitory receptors and the downregulated expression of cytotoxic molecules, which was verified in clinical samples. Further bioinformatic analysis confirmed a positive correlation between NK cell exhaustion and poor prognosis in cholecystitis and other inflammatory diseases. The study demonstrated dysfunction of liver immune cells triggered by specific metabolic abnormalities in WD, with a focus on the correlation between NK cell exhaustion and poor healing of cholecystitis, providing new insights into the improvement of inflammatory diseases by assessing immune cell function.https://elifesciences.org/articles/98867NK cell exhaustionmetabolic abnormalitiesimmune cell dysfunction |
| spellingShingle | Yong Jin Jiayu Xing Chenyu Dai Lei Jin Wanying Zhang Qianqian Tao Mei Hou Ziyi Li Wen Yang Qiyu Feng Hongyang Wang Qingsheng Yu NK cell exhaustion in Wilson’s disease revealed by single-cell RNA sequencing predicts the prognosis of cholecystitis eLife NK cell exhaustion metabolic abnormalities immune cell dysfunction |
| title | NK cell exhaustion in Wilson’s disease revealed by single-cell RNA sequencing predicts the prognosis of cholecystitis |
| title_full | NK cell exhaustion in Wilson’s disease revealed by single-cell RNA sequencing predicts the prognosis of cholecystitis |
| title_fullStr | NK cell exhaustion in Wilson’s disease revealed by single-cell RNA sequencing predicts the prognosis of cholecystitis |
| title_full_unstemmed | NK cell exhaustion in Wilson’s disease revealed by single-cell RNA sequencing predicts the prognosis of cholecystitis |
| title_short | NK cell exhaustion in Wilson’s disease revealed by single-cell RNA sequencing predicts the prognosis of cholecystitis |
| title_sort | nk cell exhaustion in wilson s disease revealed by single cell rna sequencing predicts the prognosis of cholecystitis |
| topic | NK cell exhaustion metabolic abnormalities immune cell dysfunction |
| url | https://elifesciences.org/articles/98867 |
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