DEVELOPMENT OF SPECTROPHOTOMETRICAL ASSAY FOR THE STUDY OF THE INTERACTION OF ANTIOXIDANT STANDARDS WITH 1,1-DIPHENYL-2-PICRYLHYDRAZYL FREE RADICALS
The aim of the present study was to develop a spectrophotometric based assay for the determination of binding parameters like binding constant and binding free energy of the free and 1,1-diphenyl-2-picrylhydrazyl (DPPH) bound forms of a set of antioxidant standards (AS). The determination of binding...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2015-10-01
|
| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| Subjects: | |
| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201503&vol=3&aid=4300 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The aim of the present study was to develop a spectrophotometric based assay for the determination of binding parameters like binding constant and binding free energy of the free and 1,1-diphenyl-2-picrylhydrazyl (DPPH) bound forms of a set of antioxidant standards (AS). The determination of binding constant and binding free energy is based upon the decrease in absorbance of the electronic absorption spectrum of a 10-4 M acetonitrile solution of DPPH in the presence of a given concentration of a solution of antioxidant standards in the same solvent. |
|---|---|
| ISSN: | 1582-540X 1582-540X |