Amperometric enzyme immunoassay sensors in determination of Fusarium oxysporum antigens
Enzyme immunoassay sensors (EIS) with L-cysteine desulfhydrase (CDH) as a label based on planar graphite electrodes were created to determine Fusarium oxysporum antigen (Ag). The EIS action relies on the combined effect of immunological, enzymatic, and electrochemical reactions. The immunosensors de...
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| Format: | Article |
| Language: | English |
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Kazan Federal University
2022-03-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
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| Online Access: | https://kpfu.ru/uz-eng-ns-2022-1-2.html |
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| author | R.M. Beilinson E.P. Medyantseva E.A. Kirillova E.V. Khaldeeva H.C. Budnikov |
| author_facet | R.M. Beilinson E.P. Medyantseva E.A. Kirillova E.V. Khaldeeva H.C. Budnikov |
| author_sort | R.M. Beilinson |
| collection | DOAJ |
| description | Enzyme immunoassay sensors (EIS) with L-cysteine desulfhydrase (CDH) as a label based on planar graphite electrodes were created to determine Fusarium oxysporum antigen (Ag). The EIS action relies on the combined effect of immunological, enzymatic, and electrochemical reactions. The immunosensors developed allow the determination of Fusarium oxysporum Ag in the concentration range of 5?10–10 – 5?10–6 mg/mL with the limit of quantification of 1?10–10 mg/mL. The binding constant of the Ab-Ag immune complexes was estimated as Ка1 = (6.2 ? 0.2)?109 (mg/mL)–1. No cross-reaction with Ag of such pathogenic fungi as Alternaria alternata, Cladosporium herbarum, Aspergillus fumigatus, and Aspergillus niger was confirmed. Here the quality of wheat groats was assessed to test the methods for the determination of Fusarium oxysporum Ag using the developed CDH immunosensor. |
| format | Article |
| id | doaj-art-37c9d28deedc47329072a3b0f1f6f133 |
| institution | Kabale University |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-37c9d28deedc47329072a3b0f1f6f1332025-01-02T23:28:51ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2022-03-011641223510.26907/2542-064X.2022.1.22-35Amperometric enzyme immunoassay sensors in determination of Fusarium oxysporum antigensR.M. Beilinson0E.P. Medyantseva1E.A. Kirillova2E.V. Khaldeeva3H.C. Budnikov4Kazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Research Institute of Epidemiology and Microbiology, Kazan, 420015 RussiaKazan Federal University, Kazan, 420008 RussiaEnzyme immunoassay sensors (EIS) with L-cysteine desulfhydrase (CDH) as a label based on planar graphite electrodes were created to determine Fusarium oxysporum antigen (Ag). The EIS action relies on the combined effect of immunological, enzymatic, and electrochemical reactions. The immunosensors developed allow the determination of Fusarium oxysporum Ag in the concentration range of 5?10–10 – 5?10–6 mg/mL with the limit of quantification of 1?10–10 mg/mL. The binding constant of the Ab-Ag immune complexes was estimated as Ка1 = (6.2 ? 0.2)?109 (mg/mL)–1. No cross-reaction with Ag of such pathogenic fungi as Alternaria alternata, Cladosporium herbarum, Aspergillus fumigatus, and Aspergillus niger was confirmed. Here the quality of wheat groats was assessed to test the methods for the determination of Fusarium oxysporum Ag using the developed CDH immunosensor.https://kpfu.ru/uz-eng-ns-2022-1-2.htmlfusarium oxysporumenzyme immunoassay sensorl-cysteine desulfhydrasefungal pathogens |
| spellingShingle | R.M. Beilinson E.P. Medyantseva E.A. Kirillova E.V. Khaldeeva H.C. Budnikov Amperometric enzyme immunoassay sensors in determination of Fusarium oxysporum antigens Учёные записки Казанского университета: Серия Естественные науки fusarium oxysporum enzyme immunoassay sensor l-cysteine desulfhydrase fungal pathogens |
| title | Amperometric enzyme immunoassay sensors in determination of Fusarium oxysporum antigens |
| title_full | Amperometric enzyme immunoassay sensors in determination of Fusarium oxysporum antigens |
| title_fullStr | Amperometric enzyme immunoassay sensors in determination of Fusarium oxysporum antigens |
| title_full_unstemmed | Amperometric enzyme immunoassay sensors in determination of Fusarium oxysporum antigens |
| title_short | Amperometric enzyme immunoassay sensors in determination of Fusarium oxysporum antigens |
| title_sort | amperometric enzyme immunoassay sensors in determination of fusarium oxysporum antigens |
| topic | fusarium oxysporum enzyme immunoassay sensor l-cysteine desulfhydrase fungal pathogens |
| url | https://kpfu.ru/uz-eng-ns-2022-1-2.html |
| work_keys_str_mv | AT rmbeilinson amperometricenzymeimmunoassaysensorsindeterminationoffusariumoxysporumantigens AT epmedyantseva amperometricenzymeimmunoassaysensorsindeterminationoffusariumoxysporumantigens AT eakirillova amperometricenzymeimmunoassaysensorsindeterminationoffusariumoxysporumantigens AT evkhaldeeva amperometricenzymeimmunoassaysensorsindeterminationoffusariumoxysporumantigens AT hcbudnikov amperometricenzymeimmunoassaysensorsindeterminationoffusariumoxysporumantigens |