Involvement of mediodorsal thalamus and its related neural circuit in pain regulation in mice

The mediodorsal thalamus (MD) constitutes an integral component of the medial nociceptive transmission system, although its precise role and related neural pathway in pain processing remains incompletely elucidated. While anatomical studies have confirm reciprocal projections between the MD and ante...

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Main Authors: Chu-han Liu, Hui-jie Feng, Fei Peng, Shan Wang, Liu-jie Zhao, Hui Zhu, Xue-ying Dou, Ting Yuan, Chuang Li, Nan Zhou, Wen-xin Zhang, Xuan-ren Wang, Yu-lin Dong
Format: Article
Language:English
Published: Elsevier 2025-10-01
Series:Neurobiology of Disease
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Online Access:http://www.sciencedirect.com/science/article/pii/S0969996125002724
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author Chu-han Liu
Hui-jie Feng
Fei Peng
Shan Wang
Liu-jie Zhao
Hui Zhu
Xue-ying Dou
Ting Yuan
Chuang Li
Nan Zhou
Wen-xin Zhang
Xuan-ren Wang
Yu-lin Dong
author_facet Chu-han Liu
Hui-jie Feng
Fei Peng
Shan Wang
Liu-jie Zhao
Hui Zhu
Xue-ying Dou
Ting Yuan
Chuang Li
Nan Zhou
Wen-xin Zhang
Xuan-ren Wang
Yu-lin Dong
author_sort Chu-han Liu
collection DOAJ
description The mediodorsal thalamus (MD) constitutes an integral component of the medial nociceptive transmission system, although its precise role and related neural pathway in pain processing remains incompletely elucidated. While anatomical studies have confirm reciprocal projections between the MD and anterior cingulate cortex (ACC), the functional contribution of ACC-MD neural circuitry to nociceptive regulation has not yet been systematically investigated. In this study, we used immunohistochemistry, pharmacogenetic manipulations, and multidimensional behavioral assessments to investigate the role of the MD and ACC-MD pathway in pain modulation. The MD can be activated by nociceptive stimuli, and mediates both exteroception-evoked reflexive-defensive reactions and interoception-mediated self-caring responses. Chemogenetics activation of the MD or the ACC-MD pathway induced hyperalgesia alongside aversion and anxiety-like behaviors. While inhibiting both alleviates pain and emotional comorbidities in neuropathic pain mice. These results identify a cortico-thalamic descending pathway that facilitates pain and emotional comorbidity, providing mechanistic insights for the developing neuromodulaion-based therapies against refractory neuropathic pain.
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spelling doaj-art-37bd8c5c55d847f280af7f78b6a1908a2025-08-24T05:11:30ZengElsevierNeurobiology of Disease1095-953X2025-10-0121510705610.1016/j.nbd.2025.107056Involvement of mediodorsal thalamus and its related neural circuit in pain regulation in miceChu-han Liu0Hui-jie Feng1Fei Peng2Shan Wang3Liu-jie Zhao4Hui Zhu5Xue-ying Dou6Ting Yuan7Chuang Li8Nan Zhou9Wen-xin Zhang10Xuan-ren Wang11Yu-lin Dong12Department of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Innovation Researh Institute, Xijing Hopsital, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; The Fifth Student Brigade of Basic Medical College, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Innovation Researh Institute, Xijing Hopsital, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou 450001, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Department of Human Anatomy, School of Medical Science, Yan'an University, Yan'an, Shaanxi 716000, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Department of Human Anatomy, School of Medical Science, Yan'an University, Yan'an, Shaanxi 716000, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; The First Cadets Brigade of Basic Medical College, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; The First Cadets Brigade of Basic Medical College, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; The Second Cadets Brigade of Basic Medical College, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; The Second Cadets Brigade of Basic Medical College, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; The Second Cadets Brigade of Basic Medical College, The Fourth Military Medical University, Xi'an 710032, ChinaDepartment of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China; Innovation Researh Institute, Xijing Hopsital, The Fourth Military Medical University, Xi'an 710032, China; Corresponding author at: Department of Human Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China.The mediodorsal thalamus (MD) constitutes an integral component of the medial nociceptive transmission system, although its precise role and related neural pathway in pain processing remains incompletely elucidated. While anatomical studies have confirm reciprocal projections between the MD and anterior cingulate cortex (ACC), the functional contribution of ACC-MD neural circuitry to nociceptive regulation has not yet been systematically investigated. In this study, we used immunohistochemistry, pharmacogenetic manipulations, and multidimensional behavioral assessments to investigate the role of the MD and ACC-MD pathway in pain modulation. The MD can be activated by nociceptive stimuli, and mediates both exteroception-evoked reflexive-defensive reactions and interoception-mediated self-caring responses. Chemogenetics activation of the MD or the ACC-MD pathway induced hyperalgesia alongside aversion and anxiety-like behaviors. While inhibiting both alleviates pain and emotional comorbidities in neuropathic pain mice. These results identify a cortico-thalamic descending pathway that facilitates pain and emotional comorbidity, providing mechanistic insights for the developing neuromodulaion-based therapies against refractory neuropathic pain.http://www.sciencedirect.com/science/article/pii/S0969996125002724MDACCNeuropathic painEmotional comorbidityExteroceptionInteroception
spellingShingle Chu-han Liu
Hui-jie Feng
Fei Peng
Shan Wang
Liu-jie Zhao
Hui Zhu
Xue-ying Dou
Ting Yuan
Chuang Li
Nan Zhou
Wen-xin Zhang
Xuan-ren Wang
Yu-lin Dong
Involvement of mediodorsal thalamus and its related neural circuit in pain regulation in mice
Neurobiology of Disease
MD
ACC
Neuropathic pain
Emotional comorbidity
Exteroception
Interoception
title Involvement of mediodorsal thalamus and its related neural circuit in pain regulation in mice
title_full Involvement of mediodorsal thalamus and its related neural circuit in pain regulation in mice
title_fullStr Involvement of mediodorsal thalamus and its related neural circuit in pain regulation in mice
title_full_unstemmed Involvement of mediodorsal thalamus and its related neural circuit in pain regulation in mice
title_short Involvement of mediodorsal thalamus and its related neural circuit in pain regulation in mice
title_sort involvement of mediodorsal thalamus and its related neural circuit in pain regulation in mice
topic MD
ACC
Neuropathic pain
Emotional comorbidity
Exteroception
Interoception
url http://www.sciencedirect.com/science/article/pii/S0969996125002724
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